Volume 39, Issue 1 (5-2015)                   Research in Medicine 2015, 39(1): 36-40 | Back to browse issues page

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ebrahimi N, goudarzi H, goudarzi M, asadi A, delsouz bahri T. Frequency of Alloiococus Otitidis in Patients with Otitis Media by Culture and PCR Method. Research in Medicine. 2015; 39 (1) :36-40
URL: http://pejouhesh.sbmu.ac.ir/article-1-1483-en.html
Dr Infectious Diseases and Tropical Medicine Research Center ,Shahid Beheshti University of Medical Sciences, Tehran,Iran , hgod100@yahoo.com
Abstract:   (2764 Views)

Background and Aims: Otitis media is one of the most common diseases of childhood. Bacteria and viruses can be causing these infections. The most frequently isolated organisms are, Streptococcus pneumonia, Haemophilus influenzae , Moraxella catarrhalis.

During the last decade Alloiococcus otitidis has been identified in specimens from patients with chronic otitis media with effusion. Alloiococcus otitidis is a slow growing organism, which has been isolated in a few studies on patients with otitis media with effusion. The aim of this study was to determine the presence of A. otitidis in ear discharge swabs collected from patient with OME by culture and PCR method.

Materials and Methods: A cross-sectional study was performed. Middle ear effusions from 60 people undergoing routine tympanostomy tube placement were collected.

Part of the middle ear fluid volume for microbial culture medium was inoculated and the other part was used for molecular test. Detection of A. otitidis from middle ear fluid of patients was completed by both culture and PCR method and analyzed with chi-square test.

 Results: Out of 60 samples 3 isolates (%5) were identified as A. otitidis in the middle ear fluid of the patients by culture methods and confirmed by PCR method. However, A. otitidis was detected in 28 (46%) of the middle ear fluid samples by PCR method (p<0/0001).

Conclusion: It seems that A. otitidis is the main factor causing middle ear infections and extremely difficult to detect with bacterial culture. Our study exhibited that the PCR was proper technique for detection of A. otitidis in the middle ear effusion. Further investigation is expected to reveal the clinical role of the organism in otitis media

Keywords: Alloioccocus otitidis, Otitis media, Effusion, Culture, PCR

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Type of Study: Original | Subject: Microbiology
Received: 2015/09/20 | Accepted: 2015/09/27 | Published: 2016/02/6

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