Volume 39, Number 2 (8-2015)                   Research in Medicine 2015, 39(2): 69-73 | Back to browse issues page


XML Persian Abstract Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Jafarinejad F, Bandehpour M, Gheflat S S, Kazemi B. Evaluation of Modification in M2 gene of Influenza H1N1 virus in Expression of it in BL21 Ecoli. Research in Medicine. 2015; 39 (2) :69-73
URL: http://pejouhesh.sbmu.ac.ir/article-1-1499-en.html

Associated Prof. Shahid Beheshti University of Medical Sciences , m.bandehpour@sbmu.ac.ir
Abstract:   (1985 Views)

Aim and Background: By Attention to gene sequence and each amino acid codon in host which express protein, also in order to probable vaccine production against influenza A, a major cause of mortality in a year,this research was carried out. M2 protein is conserved and it can be a proper candidate vaccine for influenza infection. Materials and Methods: Present research was an experimental study. The sequence of M2 protein of Iran influenza virus H1N1 strain was modified and synthesized. It was subcloned into pET22b vector recombinant protein and expressed in BL21 Ecoli and confirmed with specific antibody by western blot technique. Results: The modified M2 gene was expressed in Ecoli. The concentration of it was 300μg/ml and it was confirmed by western blotting with specific antibody. Conclusion: Influenza M2 protein with modification in sequence could be expressed in BL21 Ecoli host.

Full-Text [PDF 641 kb]   (742 Downloads)    
Type of Study: Original | Subject: Cellular and Molecular
Received: 2015/10/20 | Accepted: 2016/02/3 | Published: 2016/03/6

Send email to the article author


Creative Commons License
This Journal is licensed under a Creative Commons Attribution 4.0 International License  | Research in Medicine

Designed & Developed by : Yektaweb