Background: Bioaerosols are a suspended particles in air that contain organic compounds or microorganisms which moved by air flow or other physical factors and can transmit microorganisms, especially pathogens. The purpose of this study was to survey of resistance bacteria to desinfectants and antibiotics in the air at several subway stations.

Materials and Methods: In this study, sampling was performed in the air subway stations. After bacterial species identification, antibiotic resistance against various antimicrobial agents was performed by disk diffusion and broth dilution methods. The presence of qacA/B Gene was studied in sensitive and resistant S. aureus and E. coli strains by PCR methods.

Results: In the current study, the prevalence of gram negative strains were lower than gram positive strains, as well, the most strains were S. aureus and E. faecalis and the latest isolates were E. coli, respectively. The highest and lowest prevalence of qacA/B genes were S. aureus (23%) and E. coli (7%).

Conclusion: Due to the presence of pathogenic strains that can cause illness, it is necessary to cleaning to be done in subway stations using combinations of strong Cleaning needs to be done in subway stations using combinations of strong disinfectant. With regard to the availability and ease of use of sodium hypochlorite, is recommended that 5% sodium hypochlorite to use and the other detergent to be used periodically in order to  prevention of  resistant bacteria.