Abstract

Background: Breast cancer is the most common cancer among women. Polymorphisms of micro RNA genes such as microRNA 152 and microRNA 148a that they are involved in cell proliferation, angiogenesis and apoptosis could be a potential factor for increasing risk of breast cancer and its development. So, the aim of this study is to investigate the association between polymorphisms of these micro-RNAs and age of onset and progression of breast cancer.

Methods: This case-control study was conducted on 100 patients of breast cancer and 100 healthy women. The routine clinical tests were performed in studied patients regularly. After genotyping using RFLP-PCR and Tetra primer ARMS-PCR techniques, subjects of this study were categorized into three genotypes including AA,AG and GG for the miR-148a polymorphism and TT,TC and CC for the miR152 polymorphism. The collected data through the SISA website and chi-square tests were analyzed.

Findings: The results show that the miR-148a and miR152 polymorphisms reduce age of onset of cancer, but this is not statistically significant (miR152: OR=2.16, P=0.06), (miR-148a: OR=2.54, P=0.06). There is also no significant association between these polymorphisms and stages of cancer progression were observed (miR148a: OR=1.16, P=0.09); )miR152: OR=0.47, P=0.13).

Conclusion: It seems that the G and T alleles can be as a risk factor for the reduction of age of onset and breast cancer progression in Isfahan population. But to determine the alleles as biology risk factors further studies and more research is needed.