Volume 43, Issue 3 (10-2019)
Research in Medicine 2019, 43(3): 164-169 |
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Ahar Branch, Islamic Azad University, Ahar, Iran , ghorbian20@yahoo.com
Abstract: (3270 Views)
Abstract
Background: Oligospermia and azoospermia play important roles in male infertility. HIWI genes family plays an important role in spermatogenesis from spermatogonial through protection and formation of meiosis. The single-nucleotide change in HIWI2 gene has been reported as a genetic factor affecting male infertility. The aim of the present study was to investigate the relationship between HIWI2 (rs508485 C>T) genetic changes and the risk of idiopathic severe oligospermia and azoospermia in infertile men.
Materials and Methods: A case-control investigation was performed on 100 blood samples of men with idiopathic azoospermia/severe oligozoospermia and 100 blood samples of fertile men with one chilled and without historical infertility who were referred to the infertility center of Motazedi Hospital in ermanshah during 2015-2017. To determine HIWI2 C>T gene polymorphism frequency, we used Tetra ARMS-PCR method and data was analyzed using chi-squat test.
Results: In our findings, the genotype frequencies did not show a statically significant difference in dominant (P=0.306, OR=0.704), recessive (P=0.359, OR=0.786), and codominant (P>0.05, OR=1.000) heredity models. The allele frequencies did not reveal a statically significant difference between the two groups (P=0.215, OR=0.774; CI=0.516-1.161).
Conclusion: Our investigation did not show any evidence to the effect of HIWI2 rs508485 gene polymorphism as a risk factor for idiopathic azoospermia/severe oligozoospermia.
Background: Oligospermia and azoospermia play important roles in male infertility. HIWI genes family plays an important role in spermatogenesis from spermatogonial through protection and formation of meiosis. The single-nucleotide change in HIWI2 gene has been reported as a genetic factor affecting male infertility. The aim of the present study was to investigate the relationship between HIWI2 (rs508485 C>T) genetic changes and the risk of idiopathic severe oligospermia and azoospermia in infertile men.
Materials and Methods: A case-control investigation was performed on 100 blood samples of men with idiopathic azoospermia/severe oligozoospermia and 100 blood samples of fertile men with one chilled and without historical infertility who were referred to the infertility center of Motazedi Hospital in ermanshah during 2015-2017. To determine HIWI2 C>T gene polymorphism frequency, we used Tetra ARMS-PCR method and data was analyzed using chi-squat test.
Results: In our findings, the genotype frequencies did not show a statically significant difference in dominant (P=0.306, OR=0.704), recessive (P=0.359, OR=0.786), and codominant (P>0.05, OR=1.000) heredity models. The allele frequencies did not reveal a statically significant difference between the two groups (P=0.215, OR=0.774; CI=0.516-1.161).
Conclusion: Our investigation did not show any evidence to the effect of HIWI2 rs508485 gene polymorphism as a risk factor for idiopathic azoospermia/severe oligozoospermia.
Type of Study: Original |
Received: 2018/10/6 | Accepted: 2019/02/25 | Published: 2019/09/17
Received: 2018/10/6 | Accepted: 2019/02/25 | Published: 2019/09/17
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