Volume 43, Issue 1 (9-2019)
Research in Medicine 2019, 43(1): 52-57 |
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Eslami G, Taghizadeh Maleki D, Ghalavand Z, Nikmanesh B, Hashemi A, Kadkhoda H. Identification of Virulence Genes in Staphylococcus aureus Isolates Segregated from Children’s Wounds. Research in Medicine 2019; 43 (1) :52-57
URL: http://pejouhesh.sbmu.ac.ir/article-1-1935-en.html
URL: http://pejouhesh.sbmu.ac.ir/article-1-1935-en.html
Gita Eslami 
, Donya Taghizadeh Maleki , Zohreh Ghalavand , Bahram Nikmanesh , Ali Hashemi , Hiva Kadkhoda
, Donya Taghizadeh Maleki , Zohreh Ghalavand , Bahram Nikmanesh , Ali Hashemi , Hiva Kadkhoda
medical science shahid beheshti university , g_eslami@yahoo.com
Abstract: (3383 Views)
Background: Staphylococcus aureus is an opportunistic pathogenic bacterium that can provide invasive
conditions by secreting various toxins. It is involved in the development of skin infections, such as swollen
and ulcers. Today, one of the main problems is the treatment of Staphylococcus aureus resistant strains. The
aim of the current study was to detect virulence factors and antibiotic resistance among strains isolated from
wound infections of children who referred to Tehran Children’s Medical Hospital in 1396-97.
Materials and Methods: A total of 59 strains of Staphylococcus aureus isolated from wound infection were
collected during one year. Staphylococcus aureus was detected using biochemical tests. For antibiotic
susceptibility test, antibiotic disks (Mast، UK) were used according to the CLSI 2017. For detection of
virulance genes, including nuc، mecA، tsst-1، hla، hlb، hld، eta، etb، etc, PCR method was used.
Results: Vancomycin and linezolid were sensitives for all isolates and the highest resistance antibiotic was
penicillin with 98.3%. The frequency distribution of genes s mecA، tsst-1، hla، hlb، hld، eta، etb، and etd were
59.3%, 37.7%, 71.2%, 57.6%, 96.6%, 100%, 11.9%, 27.1%, respectively.
Conclusion: All isolates were sensitive to vancomycin and linezolid, and these antibiotics were the best
choices for the first line of treatment for staphylococcus aureus strains. Since all isolates of Staphylococcus
aureus were obtained from children wound infections and all of them contained oxfolitic A gene, control
and disinfection measures seem to be important among children, because these strains have the potential to
spread this gene among hospitalized patients.
conditions by secreting various toxins. It is involved in the development of skin infections, such as swollen
and ulcers. Today, one of the main problems is the treatment of Staphylococcus aureus resistant strains. The
aim of the current study was to detect virulence factors and antibiotic resistance among strains isolated from
wound infections of children who referred to Tehran Children’s Medical Hospital in 1396-97.
Materials and Methods: A total of 59 strains of Staphylococcus aureus isolated from wound infection were
collected during one year. Staphylococcus aureus was detected using biochemical tests. For antibiotic
susceptibility test, antibiotic disks (Mast، UK) were used according to the CLSI 2017. For detection of
virulance genes, including nuc، mecA، tsst-1، hla، hlb، hld، eta، etb، etc, PCR method was used.
Results: Vancomycin and linezolid were sensitives for all isolates and the highest resistance antibiotic was
penicillin with 98.3%. The frequency distribution of genes s mecA، tsst-1، hla، hlb، hld، eta، etb، and etd were
59.3%, 37.7%, 71.2%, 57.6%, 96.6%, 100%, 11.9%, 27.1%, respectively.
Conclusion: All isolates were sensitive to vancomycin and linezolid, and these antibiotics were the best
choices for the first line of treatment for staphylococcus aureus strains. Since all isolates of Staphylococcus
aureus were obtained from children wound infections and all of them contained oxfolitic A gene, control
and disinfection measures seem to be important among children, because these strains have the potential to
spread this gene among hospitalized patients.
Type of Study: Original |
Subject:
Microbiology
Received: 2018/10/15 | Accepted: 2018/11/17 | Published: 2019/04/20
Received: 2018/10/15 | Accepted: 2018/11/17 | Published: 2019/04/20
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