Volume 43, Issue 3 (10-2019)
Research in Medicine 2019, 43(3): 130-135 |
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salehiyan H, hajihosseini R, rasooli A, miri S. Comparative Effect of Three Non-Steroidal Anti-
Inflammatory Drugs on Gene Expression of Myeloperoxidase
in Lung and Liver Tissues of Septic Rats. Research in Medicine 2019; 43 (3) :130-135
URL: http://pejouhesh.sbmu.ac.ir/article-1-1961-en.html
URL: http://pejouhesh.sbmu.ac.ir/article-1-1961-en.html
, hsalehiyan230@gmail.com
Abstract: (7703 Views)
Abstract
ackground: Sepsis is the systemic response of the body to severe infection and one of the causes of
mortality in ICU. Considering the importance of sepsis, in the present study, the effect of Non-Steroidal
Anti-Inflammatory (NSAIDs) drugs (aspirin, indometacin, celecoxib) on inflammatory biomarker of
myeloperoxidase (MPO) in liver and lung tissues of rats was investigated.
Materials and Methods: In the current experimental study, animals were divided into six groups: the
control group, the lapratomy (LAP) group, the CLP group, treatment groups: CLP surgery and immediate
treatment of rats with NSAIDs (2 mg/kg b.w) once daily. Two days after CLP, the rats were killed and the
liver and lung tissues were isolated and the Real-Time PCR technique was used to determine the expression
of the MPO gene. Statistical analyses were performed using one-way ANOVA followed by LSD in SPSS
(version 19.0). The significance value was considered as P < 0.05.
Results: The results indicated that the expression of MPO gene in lung and liver tissue of the LAP group
increased significantly as compared with control group. The level of expression of the MPO gene in the
CLP lung and liver tissue was significantly increased as compared to the LAP group. However, in the
treatment groups, a significant decrease in the expression of MPO gene was observed. Although all three
drugs had a positive effect on reducing gene expression (celecoxib>indomethacin>aspirin), in lung tissue,
celecoxib had the highest effect and aspirin had the least effect on reducing the expression of MPO gene.
In liver tissue, aspirin, and celecoxib, the least effect on reducing gene expression was observed.
Conclusion: It seems that the treatment of animals with selected drugs have the inhibiting effect on the
increase of MPO gene expression and results in reducing the damage to the liver and lung tissue caused
by sepsis.
ackground: Sepsis is the systemic response of the body to severe infection and one of the causes of
mortality in ICU. Considering the importance of sepsis, in the present study, the effect of Non-Steroidal
Anti-Inflammatory (NSAIDs) drugs (aspirin, indometacin, celecoxib) on inflammatory biomarker of
myeloperoxidase (MPO) in liver and lung tissues of rats was investigated.
Materials and Methods: In the current experimental study, animals were divided into six groups: the
control group, the lapratomy (LAP) group, the CLP group, treatment groups: CLP surgery and immediate
treatment of rats with NSAIDs (2 mg/kg b.w) once daily. Two days after CLP, the rats were killed and the
liver and lung tissues were isolated and the Real-Time PCR technique was used to determine the expression
of the MPO gene. Statistical analyses were performed using one-way ANOVA followed by LSD in SPSS
(version 19.0). The significance value was considered as P < 0.05.
Results: The results indicated that the expression of MPO gene in lung and liver tissue of the LAP group
increased significantly as compared with control group. The level of expression of the MPO gene in the
CLP lung and liver tissue was significantly increased as compared to the LAP group. However, in the
treatment groups, a significant decrease in the expression of MPO gene was observed. Although all three
drugs had a positive effect on reducing gene expression (celecoxib>indomethacin>aspirin), in lung tissue,
celecoxib had the highest effect and aspirin had the least effect on reducing the expression of MPO gene.
In liver tissue, aspirin, and celecoxib, the least effect on reducing gene expression was observed.
Conclusion: It seems that the treatment of animals with selected drugs have the inhibiting effect on the
increase of MPO gene expression and results in reducing the damage to the liver and lung tissue caused
by sepsis.
Type of Study: Original |
Subject:
Biochemistry
Received: 2018/12/24 | Accepted: 2019/03/13 | Published: 2019/09/17
Received: 2018/12/24 | Accepted: 2019/03/13 | Published: 2019/09/17
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