Volume 21, Issue 1 (June 1997)
Research in Medicine 1997, 21(1): 67-79 |
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Mehbod A S A, Shahabi S, Hatami M. Growth of Pneumocystis Carinii in Axenic Culture. Research in Medicine 1997; 21 (1) :67-79
URL: http://pejouhesh.sbmu.ac.ir/article-1-2264-en.html
URL: http://pejouhesh.sbmu.ac.ir/article-1-2264-en.html
Parasite & Mycology Department, College of Medicine Shaheed Beheshti University of Medical Sciences & Health Services,Iran.
Abstract: (1584 Views)
SUMMARY
In this research, which was done for two years, we investigated various liquid and solid media without feeder ceells, for the ability to support growth of P. carinii. Data obtained from counting the organisms nuclei showed that, addition of Cycloheximide, penicillin, & streptomycin to neopeptoneN-Acetyle Glucoseamine (N.P.G. medium) can provides growth requirements of organism in its trophozoites form. We found that, preculture adjacence of P. carinii with group A streptococci or addition of some reducing substances, or vitamines to N.P.G. would increase the growth rate of organism.
It was also shown that, addition of Neopeptone, N-Acetyl-Glucoseamine, and lowering its PH to 4-0, Rosewell Park Memorial Institute medium (R.P.M.I.), can also provides organisms growth. And also addition of agar & humans blood group Oto N.P.G. can enable P carinii to growth on the slant, and produce micro colonies.
In this research, which was done for two years, we investigated various liquid and solid media without feeder ceells, for the ability to support growth of P. carinii. Data obtained from counting the organisms nuclei showed that, addition of Cycloheximide, penicillin, & streptomycin to neopeptoneN-Acetyle Glucoseamine (N.P.G. medium) can provides growth requirements of organism in its trophozoites form. We found that, preculture adjacence of P. carinii with group A streptococci or addition of some reducing substances, or vitamines to N.P.G. would increase the growth rate of organism.
It was also shown that, addition of Neopeptone, N-Acetyl-Glucoseamine, and lowering its PH to 4-0, Rosewell Park Memorial Institute medium (R.P.M.I.), can also provides organisms growth. And also addition of agar & humans blood group Oto N.P.G. can enable P carinii to growth on the slant, and produce micro colonies.
Type of Study: Original |
Subject:
Interdisciplinary (Educational Management, Educational research, Statistics, Medical education
Received: 2020/02/18 | Accepted: 2020/02/18 | Published: 2020/02/18
Received: 2020/02/18 | Accepted: 2020/02/18 | Published: 2020/02/18
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