fa بررسی نقش بیان ژن ACVR2A با جفت پره‌اکلامپسی ژنتیک Genetic پژوهشی Original <span style="font-size:11pt"><span style="line-height:110%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><span lang="AR-SA" style="line-height:110%"><span b="" nazanin="" style="font-family:">سابقه و هدف</span></span></b><b><span b="" lang="AR-SA" nazanin="" style="font-family:">: </span></b><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:110%"><span b="" nazanin="" style="font-family:">پره‌اکلامپسی یکی از بیماری‌های مهم در زمان بارداری است و در صورت عدم رسیدگی، احتمال مرگ مادر و جنین افزایش می‌یابد. جفت غیرطبیعی به‌طورکلی به‌عنوان دلیل اولیه این اختلال پذیرفته</span></span></span> <span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:110%"><span b="" nazanin="" style="font-family:">‌شده است. افزایش بیان اکتوین</span></span></span><span dir="LTR" style="font-size:9.0pt"><span style="line-height:110%"><span new="" roman="" style="font-family:" times=""> A </span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:110%"><span b="" nazanin="" style="font-family:">که نقش مهمی در رشد و ایجاد جفت و جنین ایفا می‌کند در بیماران پره اکلمپسی تأیید ‌شده است، اما مکانیسم عمل گیرنده اکتیوین </span></span></span><span dir="LTR" style="font-size:9.0pt"><span style="line-height:110%"><span new="" roman="" style="font-family:" times="">A</span></span></span> <span dir="LTR" style="font-size:9.0pt"><span style="line-height:110%"><span new="" roman="" style="font-family:" times="">(ACVR2A)</span></span></span><span style="font-size:10.0pt"><span style="line-height:110%"><span b="" nazanin="" style="font-family:"> ناشناخته است؛ به همین دلیل در این مطالعه بیان ژن </span></span></span><span dir="LTR" style="font-size:9.0pt"><span style="line-height:110%"><span new="" roman="" style="font-family:" times="">ACVR2A</span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:110%"><span b="" nazanin="" style="font-family:"> درجفت زنان مبتلا به پره اکلامپسی در مقایسه با جفت زنان باردار سالم بررسی شد.</span></span></span><span lang="AR-SA" style="line-height:110%"><span style="font-family:"B Nazanin""></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:110%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><span lang="AR-SA" style="line-height:110%"><span b="" nazanin="" style="font-family:">روش کار:</span></span></b> <span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:110%"><span b="" nazanin="" style="font-family:">در این مطالعه مورد-</span></span></span> <span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:110%"><span b="" nazanin="" style="font-family:">شاهد، بیان ژن </span></span></span><span dir="LTR" style="font-size:9.0pt"><span style="line-height:110%"><span new="" roman="" style="font-family:" times="">ACVR2A</span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:110%"><span b="" nazanin="" style="font-family:"> در 40 نمونه جفت، شامل 20 نمونه جفت از زنان باردار مبتلابه پره‌اکلامپسی و 20</span></span></span> <span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:110%"><span b="" nazanin="" style="font-family:">نمونه جفت از خانم‌های باردار سالم با استفاده از روش </span></span></span><span dir="LTR" style="font-size:9.0pt"><span style="line-height:110%"><span new="" roman="" style="font-family:" times="">Real- Time PCR</span></span></span> <span dir="LTR" style="font-size:9.0pt"><span style="line-height:110%"><span new="" roman="" style="font-family:" times="">Q</span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:110%"><span b="" nazanin="" style="font-family:">، توسط نرم‌افزار </span></span></span><span dir="LTR" style="font-size:9.0pt"><span style="line-height:110%"><span new="" roman="" style="font-family:" times="">REST</span></span></span><span style="font-size:10.0pt"><span style="line-height:110%"><span b="" nazanin="" style="font-family:"> تغییرهای بیان بین دو گروه بررسی شد و نمودار تغییرهای بیان با استفاده از آزمون آماری </span></span></span><span dir="LTR" style="font-size:9.0pt"><span style="line-height:110%"><span new="" roman="" style="font-family:" times="">T-test</span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:110%"><span b="" nazanin="" style="font-family:"> و </span></span></span><span dir="LTR" style="font-size:9.0pt"><span style="line-height:110%"><span new="" roman="" style="font-family:" times="">Mann- Whitney</span></span></span><span style="font-size:10.0pt"><span style="line-height:110%"><span b="" nazanin="" style="font-family:"> در نرم‌افزار </span></span></span><span dir="LTR" style="font-size:9.0pt"><span style="line-height:110%"><span new="" roman="" style="font-family:" times="">GraphPad Prism</span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:110%"><span b="" nazanin="" style="font-family:"> رسم شد.</span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"Times New Roman",serif"></span></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:110%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><span lang="AR-SA" style="line-height:110%"><span b="" nazanin="" style="font-family:">یافته</span></span></b><b><span dir="LTR" style="font-size:10.0pt"><span style="line-height:110%">‌</span></span></b><b><span lang="AR-SA" style="line-height:110%"><span b="" nazanin="" style="font-family:">ها:</span></span></b> <span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:110%"><span b="" nazanin="" style="font-family:">افزایش بیان ژن </span></span></span><span dir="LTR" style="font-size:9.0pt"><span style="line-height:110%"><span new="" roman="" style="font-family:" times="">ACVR2A</span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:110%"><span b="" nazanin="" style="font-family:"> درجفت زنان مبتلابه پره اکلامپسی نسبت به جفت طبیعی مشاهده شد. جفت گروه پره اکلامپسی 5/5 برابر افزایش بیان در مقایسه با گروه کنترل نشان داد.</span></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:110%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><span lang="AR-SA" style="line-height:110%"><span b="" nazanin="" style="font-family:">نتیجه</span></span></b><b><span dir="LTR" style="font-size:10.0pt"><span style="line-height:110%">‌</span></span></b><b><span lang="AR-SA" style="line-height:110%"><span b="" nazanin="" style="font-family:">گیری</span></span></b><span lang="AR-SA" style="line-height:110%"><span b="" nazanin="" style="font-family:">:</span></span> <span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:110%"><span b="" nazanin="" style="font-family:">به نظر می‌رسد که</span></span></span> <span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:110%"><span b="" nazanin="" style="font-family:">افزایش بیان ژن </span></span></span><span dir="LTR" style="font-size:9.0pt"><span style="line-height:110%"><span new="" roman="" style="font-family:" times="">ACVR2A</span></span></span><span style="font-size:10.0pt"><span style="line-height:110%"><span b="" nazanin="" style="font-family:"> با بروز پره اکلامپسی نقش دارد.</span></span></span><span lang="AR-SA" style="line-height:110%"><span style="font-family:"B Nazanin""></span></span></span></span></span></span></span> <span style="font-size:11pt"><span style="line-height:110%"><span style="font-family:Calibri,sans-serif"><b><span style="font-size:10.0pt"><span style="line-height:110%"><span new="" roman="" style="font-family:" times="">Background and Aim:</span></span></span></b> <span style="font-size:10.0pt"><span style="line-height:110%"><span new="" roman="" style="font-family:" times="">Preeclampsia is a significant condition during pregnancy, and if left untreated, it increases the risk of maternal and fetal mortality. Abnormal placentation is widely recognized as the primary cause of this disorder. Increased expression of Activin A, which plays a crucial role in placental and fetal development, has been confirmed in patients with preeclampsia. However, the mechanism of action of the Activin A receptor (ACVR2A) remains unclear. Therefore, this study examined the expression of the ACVR2A gene in the placentas of women with preeclampsia compared to those of healthy pregnant women.<span style="background:white"></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:110%"><span style="font-family:Calibri,sans-serif"><b><span style="font-size:10.0pt"><span style="background:white"><span style="line-height:110%"><span new="" roman="" style="font-family:" times="">Methods:</span></span></span></span></b> <span style="font-size:10.0pt"><span style="line-height:110%"><span new="" roman="" style="font-family:" times="">In this case- control study, the expression of the ACVR2A gene in 40 placental samples, including 20 from women with preeclampsia and 20 from healthy pregnant women, was analyzed using quantitative Real- Time PCR (qPCR). The expression changes between the two groups were assessed with REST software, and the expression variation charts were plotted using T-test and Mann- Whitney statistical tests in GraphPad Prism software.<span style="background:white"></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:110%"><span style="font-family:Calibri,sans-serif"><strong><span style="font-size:10.0pt"><span style="background:white"><span style="line-height:110%"><span new="" roman="" style="font-family:" times="">Results</span></span></span></span></strong><b><span style="font-size:10.0pt"><span style="background:white"><span style="line-height:110%"><span new="" roman="" style="font-family:" times="">:</span></span></span></span></b> <span style="font-size:10.0pt"><span style="line-height:110%"><span new="" roman="" style="font-family:" times="">increased expression of the ACVR2A gene was observed in the placentas of women with preeclampsia compared to normal placentas . The placenta in the preeclampsia group showed a 5.5-fold increase in gene expression compared to the control group.<span style="background:white"></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:110%"><span style="font-family:Calibri,sans-serif"><b><span style="font-size:10.0pt"><span style="line-height:110%"><span new="" roman="" style="font-family:" times="">Conclusion:</span></span></span></b> <span style="font-size:10.0pt"><span style="line-height:110%"><span new="" roman="" style="font-family:" times="">It appears that the upregulation of ACVR2A gene expression plays a role in the development of preeclampsia.</span></span></span><span style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"Times New Roman",serif"></span></span></span></span></span></span> پره‌اکلامپسی؛ ژنACVR2A؛ بیان؛ جفت؛ بارداری؛ فشارخون Preeclampsia, ACVR2A gene, expression, placenta, pregnancy, hypertension 1 8 http://pejouhesh.sbmu.ac.ir/browse.php?a_code=A-10-4892-1&slc_lang=fa&sid=1 Asal Honarpour عسل هنرپور 100319475328460032180 100319475328460032180 No Department of Genetics, North Tehran Branch, Iran Islamic Azad University, Tehran, Iran. گروه زیست‌شناسی ژنتیک، دانشکده علوم زیستی، دانشگاه آزاد اسلامی واحد تهران شمال، تهران، ایران. Ahmad Majd احمد مجد Ahmad_majd2005@yahoo.com 100319475328460032181 100319475328460032181 Yes Department of Biology, North Tehran Branch, Iran Islamic Azad University, Tehran, Iran. گروه‌زیست‌شناسی، دانشکده علوم زیستی، دانشگاه آزاد اسلامی واحد تهران شمال، تهران، ایران. Reza Mirfakhraie رضا میرفخرایی 100319475328460032182 100319475328460032182 No Departments of Medical Genetics, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran. گروه ژنتیک پزشکی، دانشکده پزشکی، دانشگاه علوم پزشکی شهید بهشتی، تهران، ایران. Seyed Hamid Jamaldini سید حمید جمال الدینی 100319475328460032183 100319475328460032183 No Medical Genomics Research Center, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran. مرکز تحقیقات ژنومیک پزشکی، دانشگاه علوم پزشکی آزاد اسلامی تهران، تهران، ایران. Maryam Rahimi مریم رحیمی Mrahimi.gynecologist@gmail.com 100319475328460032184 100319475328460032184 No Department of Gynecology and Obstetrics, School of Medicine, Iran University of Medical Sciences, Tehran, Iran. گروه زنان و زایمان دانشکده پزشکی، دانشگاه علوم پزشکی ایران، تهران، ایران. Sina Salimi Nasab سینا سلیمی نسب 100319475328460032185 100319475328460032185 No Department of Plastic and Reconstructive Surgery, 15th Khordad Hospital, Tehran, Iran. گروه جراح پلاستیک، دانشکده پزشکی، دانشگاه علوم پزشکی شهید بهشتی، تهران، ایران.