Background: The present study aimed to examine the effect of aerobic training on Klotho protein, interleukin-6 and tumor necrosis factor alpha inflammatory factors in rats.

Materials and Methods: For this purpose 12 male rats with an average weight 170g±5g and the age of eight weeks were divided into two groups: control (N= 6) and experimental (N= 6) groups. The experimental groups trained on a treadmill for 8 weeks and 3 sessions per week.  48 h after last training session, both groups of rats were slaughtered ethically and kidney tissue were extracted. Klotho, IL-6 and TNF-α proteins of kidney tissues were measured and counted using by western blot method. 

Results: The Klotho protein (P = 0/05) significantly increased in trained rats compared with the control group. Also the IL-6 protein (P = 0/05) significantly decreased in trained rats compared with the control group. While the TNF-α protein (P = 0/42) did not show a significant difference.

Conclusion: aerobic training might increase the Klotho protein, on the other hand can also decrease the levels of IL-6 protein secreted by kidney. So it can be said that probably aerobic training may delay aging symptoms and may decrease in inflammatory responses.

Keywords: Aerobic training, Klotho, TNF-α, IL6