Volume 43, Issue 1 (9-2019)
Research in Medicine 2019, 43(1): 1-7 |
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Mosaffa N, Rahmani H, Sadeghi S, Roshani M, Amani D, Ghazanfari T. Particulate matter effects on peritoneal macrophages inflammatory function in C57BL/6 mice. Research in Medicine 2019; 43 (1) :1-7
URL: http://pejouhesh.sbmu.ac.ir/article-1-1918-en.html
URL: http://pejouhesh.sbmu.ac.ir/article-1-1918-en.html
Shahid beheshti university of medical science , mosaffan@sbmu.ac.ir
Abstract: (4128 Views)
Abstract
Background: Urban air pollution represents a challenge in Tehran. Health care organs and physicians
pay much attention because of health effect on mankind. Nowadays, a majority of air pollution
related articles focus on particulate matter adverse effects on human health. Epidemiologic studies
show the direct correlation between ambient particulate matter and risk of respiratory system and
cardiovascular disease. However, little attention has been given to the effects of air pollution on
the gastrointestinal system. Emergency system visit increases on polluted days. Recent studies have
shown high incidence of susceptibility to inflammatory bowel diseases (IBD), appendicitis, and
colorectal cancer in the polluted ar eas.
Materials and Methods: We investigated the effect of Particulate matter in two aerodynamic
diameters (PM2.5 and PM10) on peritoneal immune system cells. PM filters of different stations
were purchased from Tehran Air Quality Standard Agency. After harvesting the particles from
corresponding filters, the PMs were cultured with C57BL/6 inbred mice peritoneal macrophages.
MTT test was performed to define the stimulatory and toxic effects of PMs on macrophages.
Results: The results of MTT test showed stimulation of macrophages by PMs. PM2.5 was more
potent in increasing MTT test absorbance. Also, both particles stimulated macrophages in 10μg/ml
concentration in comparison to 5 μg/ml concentration.
Conclusion: These results show particles stimulate macrophages and this stimulation can result in
production of inflammatory cytokines and other macrophage function. In further studies, we will
continue our investigation by evaluating PM effects on pr oducing cytokines and gene expression.
Keywords: Immune system; Macrophage; Peritoneal; Suspended particles of air pollution
Background: Urban air pollution represents a challenge in Tehran. Health care organs and physicians
pay much attention because of health effect on mankind. Nowadays, a majority of air pollution
related articles focus on particulate matter adverse effects on human health. Epidemiologic studies
show the direct correlation between ambient particulate matter and risk of respiratory system and
cardiovascular disease. However, little attention has been given to the effects of air pollution on
the gastrointestinal system. Emergency system visit increases on polluted days. Recent studies have
shown high incidence of susceptibility to inflammatory bowel diseases (IBD), appendicitis, and
colorectal cancer in the polluted ar eas.
Materials and Methods: We investigated the effect of Particulate matter in two aerodynamic
diameters (PM2.5 and PM10) on peritoneal immune system cells. PM filters of different stations
were purchased from Tehran Air Quality Standard Agency. After harvesting the particles from
corresponding filters, the PMs were cultured with C57BL/6 inbred mice peritoneal macrophages.
MTT test was performed to define the stimulatory and toxic effects of PMs on macrophages.
Results: The results of MTT test showed stimulation of macrophages by PMs. PM2.5 was more
potent in increasing MTT test absorbance. Also, both particles stimulated macrophages in 10μg/ml
concentration in comparison to 5 μg/ml concentration.
Conclusion: These results show particles stimulate macrophages and this stimulation can result in
production of inflammatory cytokines and other macrophage function. In further studies, we will
continue our investigation by evaluating PM effects on pr oducing cytokines and gene expression.
Keywords: Immune system; Macrophage; Peritoneal; Suspended particles of air pollution
Type of Study: Original |
Subject:
Immunology
Received: 2018/09/5 | Accepted: 2018/12/23 | Published: 2019/04/20
Received: 2018/09/5 | Accepted: 2018/12/23 | Published: 2019/04/20
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