Volume 44, Issue 1 (3-2020)                   Research in Medicine 2020, 44(1): 308-313 | Back to browse issues page

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latifi A, NIYYATI M, GAJARI A, lasjerdi Z. Isolation and molecular identification of free-living amoeba from hot springs in the city of Mahallat located in the Central Province. Research in Medicine. 2020; 44 (1) :308-313
URL: http://pejouhesh.sbmu.ac.ir/article-1-1992-en.html
, maryamniyati@yahoo.com
Abstract:   (1978 Views)
Background: Free living amoebae include many genera such as Acanthamoeba, Naegleria, Balamuthia and sapinia. These opportunistic FLA could be the causal agent of various diseases including Keratitis, Encephalitis and Cutaneous ulcers. These amoebae could be present in water sources. the aim of the present study is to isolate FLA from Hot springs samples collected from various districts in hot springs of Mahallat located in the Central Province by employing morphological and molecular method. The presnet study was conducted for the first time in summer 2016.
Methods: In this study, 87 samples were collected from 5 hot springs , 24 pools and small pools. Three samples with 500 ml volume were taken in sterile bottles and transferred to laboratory of Shahid Beheshti University of Medical Sciences. Based on culture and morphological and molecular criteria, hot springs contamination to free living amoebae was examined.
Results: Out of 77 collected samples, 33 (42.85%) samples were contaminated to free living amoebae. The most prevalent amoebae were Vermamoebae vermiformis 18 (54.54%), Acanthamoeba isolates belonging to T4 12 (36.36%) and Naegleria australiensis  3 (9.09%).
Conclusion: The results indicated the presence of potentially pathogenic Vermamoebae vermiformis, Acanthamoeba isolates belonging to T4 and Naegleria australiensis  in Hot springs of Mahallat located in the Central Province. Improved filtration and posting alarm signs are necessary to exclude such amoebae from Hot springs. It seems hot springs contamination to free living amoebae is relevant and this could be a health hazard for endemic people, tourists and travelers who use these hot springs. Improved disinfection and filteration of hot spring is important for prevention of diseases that are caused Such as Keratitis, Encephalitis and Cutaneous ulcers.
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Type of Study: Original | Subject: Parasitology
Received: 2019/03/2 | Accepted: 2019/06/15 | Published: 2020/04/11

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