Abstract
Background & aim: Colorectal cancer as a leading cause of cancer related death develops through multistep pathway. MicroRNAs (microRNAs) are a group of small noncoding RNAs that inhibit target mRNA translation. Studies show that abnormal expression of these genes is associated with colorectal cancer. Studies have shown that abnormal expression of these genes is associated with colorectal cancer. miR-195 appears to be the major regulatory factor in various cancers including colorectal cancer. Further studies are needed to precisely determine the regulatory role of miR-195 in colorectal cancer. The aim of this study was to determine the expression level of miR-195 in colon adenocarcinoma and normal tissue specimens and to confirm this biomarker in tissue samples in Iran.
Materials and Methods: The study was a case-control design. In vitro bioinformatics analysis was performed on gene expression and its results were confirmed by experimental and in vitro analysis on clinical samples. In this study, after RNA extraction and cDNA sequencing, gene expression was designed using primers. Real time PCR was measured and then statistically analyzed.
Results: The initiation of invasion and metastasis pathways may be due to decreased miR-195 expression. The qRT-PCR test results for the expression of genes showed that mi3R-195 expression was potentially decreased in tumor tissues compared to adjacent normal tissues (p <0.00001). miR-195 is potentially associated with clinicopathologic features of tumors (P value 0.2363). Conclusion: The present study demonstrates the potential role of 195 - miR in the development of colon cancer.