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Mashhad Branch, Islamic Azad University , shahrokhabady@yahoo.com
Abstract:   (555 Views)
Background & Aim: Cancer is the leading cause of death after cardiovascular disease and accidents. Nowadays, researchers are increasing their efforts to use treatments with minimal side effects and trying to use traditional medicine and herbal remedies. AnbarNesara is a seemingly worthless substance found in traditional medicine and used in the treatment of many diseases. The aim of this study was to evaluate the cytotoxic effects of the smoke from the burning of AnbarNesara on liver cancer cells and to investigate the genes expression changes of P53 and CYP2E1.
Materials and Methods: In this experimental study, first Anbarnsara was collected from Dargaz region in spring and then the smoke from its fuel was collected in cold propyleneglycol. The amount of smoke in the solvent was calculated. Liver cancer cell line (HEPG2) and normal cell line were cultured. Cytotoxicity tests and morphological studies were performed and IC50 value was calculated. RNA extraction, cDNA synthesis and Real Time PCR were performed to evaluate the expression of P53 and CYP2E1 genes. The results were analyzed by t-test and ANOVA at a significant level (P <0.05).
Results: The results of morphological studies and toxicity test indicate that smoke from AnbarNesara fuel at a concentration of 58.5 mg/ml increases cancer cell mortality (IC50=48.79) compared to the control group (P <0.05). In the study of gene expression, while the concentration of 117 mg increased the expression of CYP2E1 gene, the P53 gene increased at the concentration of 58.5 (P <0.05).
Conclusion: The smoke from burning Anbarnsara seems to have a lethal effect on liver cancer cells. Duration and concentration are effective in this effectiveness.
 
     
Type of Study: Original | Subject: Genetic
Received: 2020/01/15 | Accepted: 2020/08/25

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