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Shahid Beheshti University of Medical Sciences , Janahmadi@sbmu.ac.ir
Abstract:   (439 Views)
Background and aims: Epilepsy is one of the most common neurological diseases in the world. Intracellular recording from neurons with epileptiform activity has well shown the burst firing pattern. Previous studies have shown that melatonin anti-epileptic effects, however, its effect on the neuronal properties and altering the firing pattern has not been determined yet. Therefore, in the present study, the effect of melatonin on the electrophysiological parameters in an epileptic model induced by pentylenetetrazole (PTZ) has been investigated.
Summary of Method: This study was experimentally performed on the soma membrane of F1 neurons of sub-esophageal ganglia of Helix aspersa (Iranian garden snail). The firing pattern and action potential were recorded using a single electrode intracellular recording under control, epileptiform activity induced by pentylenetetrazole, and treatment with melatonin (100µM) conditions. Data analysis was done using Prism (version 8, GraphPad Software, Inc.), and one- way ANOVA followed by Tukey’s test was used.
Results: The present findings showed that melatonin at concentration of 100µM significantly (P<0.001) prevented the depolarizing effect of pentylenetetrazole on resting membrane potential. In addition, it caused a significant (P<0.001) reduction in firing frequency and an increase in the afterhyperpolarization potential. Furthermore, it resulted in changes in the electrical firing activity of neurons from bursting in the present of PTZ to a tonic firing in the control condition.
Discussion and Conclusion: Findings of intracellular recording showed that melatonin by altering the electrophysiological parameters caused a reduction of epileptiform activity and changing the bursting pattern due to PTZ to a tonic pattern. It seems melatonin can be effective in the treatment of epilepsy by reducing neuronal excitability.  
Type of Study: Original | Subject: Physilogy
Received: 2020/01/19 | Accepted: 2020/08/25

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