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Abstract:   (434 Views)
Background: Staphylococcus aureus is a major cause of nosocomial infections in the world. Efflux pumps are well known as a key role to fluoroquinolone resistance in methicillin-resistant Staphylococcus aureus (MRSA).  This study was performed to determine resistance to antimicrobial agents related to efflux pumps isolates recovered from blood cultures of patients referred to several hospitals in Shahid Beheshti university between 1396-1397. 
 
Materials and Methods: In the present descriptive cross-sectional study, A total of 100 clinical samples were collected from blood cultures over a 12-month period. S. aureus isolates were identified by standard microbiological tests. Antimicrobial susceptibility patterns were determined by disk diffusion method and micro-dilution method. Subsequently, the presence of norA efflux pump gene was detected using PCR method. Finally, active efflux pumps were evaluated using ciprofloxacin and ethidium bromide MICs. Polymerase chain reaction (PCR) and sequencing were used to investigate the norA efflux pump gene. Chi- square test was used for statistical analyses.
Results: Among 100 clinical samples, 38 of 45 MRSA isolates (84.4%) showed resistance to ciprofloxacin. Moreover, the norA gene was found in 100% of ciprofloxacin resistant isolates. Among 38 ciprofloxacin-resistant isolates 8 isolates showed active efflux activity by reducing EtBr and ciprofloxacin MIC values by 2 to 4 folds, in the presence of CCCP (carbonyl cyanide 3-chlorophenylhydrazone).
Conclusion:
It seems a rising trend of antimicrobial resistance among S.aureus isolates to different antibiotics has  become a widespread concern in infection control.
     
Type of Study: Original | Subject: Microbiology
Received: 2020/05/31 | Accepted: 2020/09/8

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