Pejouhesh dar Pezeshki (Research in Medicine) پژوهش در پزشکی Research in Medicine Medical Sciences http://pejouhesh.sbmu.ac.ir 1 journal1 1735-5311 2008-0506 fa jalali 1401 6 1 gregorian 2022 9 1 46 3 online 1 fulltext
fa بررسی اثر مهاری نانوذره روی بر روی باکتری سودوموناس آئروژینوزا Investigation of the Inhibitory Effect of Zinc Nanoparticles on Pseudomonas aeruginosa میکروب‌شناسی Microbiology پژوهشی Original <div style="text-align: justify;"><span style="font-size:11pt"><span style="line-height:112%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><span lang="AR-SA" style="line-height:112%"><span b="" nazanin="" style="font-family:">سابقه و هدف</span></span></b><b><span b="" lang="AR-SA" nazanin="" style="font-family:">: </span></b><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:112%"><span b="" nazanin="" style="font-family:"><span style="color:black">امروزه، استفاده از فلزات نظیر نقره، مس، طلا و آلومینیوم در فرم‌های مختلف از‌جمله نانوذرات به عنوان ترکیباتی ضد‌میکروبی و باکتری‌کش رواج پیدا کرده است. </span></span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:112%"><span b="" nazanin="" style="font-family:"><span style="color:black">ا</span></span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:112%"><span b="" nazanin="" style="font-family:"><span style="color:black">ین مطالعه به منظور بررسی اثر نانوذره روی بر باکتری <i>‌سودوموناس آئروژینوزا</i> </span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:112%"><span new="" roman="" style="font-family:" times=""><span style="color:black">(ATCC 27853)</span></span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:112%"><span b="" nazanin="" style="font-family:"><span style="color:black"> انجام گرفت.</span></span></span></span><span lang="AR-SA" style="line-height:112%"><span style="font-family:"B Nazanin""></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:112%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><span lang="AR-SA" style="line-height:112%"><span b="" nazanin="" style="font-family:">روش کار:</span></span></b> <span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:112%"><span b="" nazanin="" style="font-family:"><span style="color:black">در این مطالعه تجربی نانوذره روی از شر‌‌کت نانو پارس، تهران تهیه شد. از باکتری <i>‌سودوموناس&shy; آئروژینوزا</i> (</span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:112%"><span new="" roman="" style="font-family:" times=""><span style="color:black">ATCC 27853</span></span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:112%"><span b="" nazanin="" style="font-family:"><span style="color:black">)</span></span></span></span> <span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:112%"><span b="" nazanin="" style="font-family:"><span style="color:black">به‌عنوان میکروارگانیسم مورد آزمایش استفاده شد. مقایسه فعالیت ضدباکتریایی نانو ذره روی با بنزآلکونیوم با استفاده از روش انتشار در آگار (دیسک دیفیوژن) و</span></span></span></span>&nbsp;<span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:112%"><span b="" nazanin="" style="font-family:"><span style="color:black">&nbsp;رقت سازی سریالی (دایلوشن) بررسی شد. داده‌های</span></span></span></span> <span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:112%"><span b="" nazanin="" style="font-family:"><span style="color:black">به</span></span></span></span> <span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:112%"><span b="" nazanin="" style="font-family:"><span style="color:black">دست</span></span></span></span> <span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:112%"><span b="" nazanin="" style="font-family:"><span style="color:black">آمده،</span></span></span></span> <span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:112%"><span b="" nazanin="" style="font-family:"><span style="color:black">توسط</span></span></span></span> <span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:112%"><span b="" nazanin="" style="font-family:"><span style="color:black">نرم‌افزار </span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:112%"><span new="" roman="" style="font-family:" times=""><span style="color:black">spss</span></span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:112%"><span b="" nazanin="" style="font-family:"><span style="color:black"> ورژن 22 </span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:112%"><span new="" roman="" style="font-family:" times=""><span style="color:black">(IBM, Armonk, NY, USA)</span></span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:112%"><span b="" nazanin="" style="font-family:"><span style="color:black"> و</span></span></span></span> <span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:112%"><span b="" nazanin="" style="font-family:"><span style="color:black">با</span></span></span></span> <span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:112%"><span b="" nazanin="" style="font-family:"><span style="color:black">استفاده</span></span></span></span> <span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:112%"><span b="" nazanin="" style="font-family:"><span style="color:black">از</span></span></span></span> <span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:112%"><span b="" nazanin="" style="font-family:"><span style="color:black">روش</span></span></span></span> <span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:112%"><span b="" nazanin="" style="font-family:"><span style="color:black">آماری</span></span></span></span> <span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:112%"><span b="" nazanin="" style="font-family:"><span style="color:black">آنالیز</span></span></span></span> <span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:112%"><span b="" nazanin="" style="font-family:"><span style="color:black">واریانس یکطرفه (</span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:112%"><span new="" roman="" style="font-family:" times=""><span style="color:black">One way ANOVA</span></span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:112%"><span b="" nazanin="" style="font-family:"><span style="color:black">) و آزمون توکی تجزیه</span></span></span></span> <span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:112%"><span b="" nazanin="" style="font-family:"><span style="color:black">و</span></span></span></span> <span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:112%"><span b="" nazanin="" style="font-family:"><span style="color:black">تحلیل</span></span></span></span> <span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:112%"><span b="" nazanin="" style="font-family:"><span style="color:black">آماری</span></span></span></span> <span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:112%"><span b="" nazanin="" style="font-family:"><span style="color:black">&nbsp;شد.</span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:112%"><span style="font-family:"Times New Roman",serif"></span></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:112%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><span lang="AR-SA" style="line-height:112%"><span b="" nazanin="" style="font-family:">یافته</span></span></b><b><span dir="LTR" style="font-size:10.0pt"><span style="line-height:112%">‌</span></span></b><b><span lang="AR-SA" style="line-height:112%"><span b="" nazanin="" style="font-family:">ها:</span></span></b> <span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:112%"><span b="" nazanin="" style="font-family:"><span style="color:black">نتایج حاکی از آن بود که با اضافه شدن نانو ذرات روی به حلال بنزآلکونیوم کلراید میزان قطر‌ هاله عدم رشد باکتری <i>‌سودوموناس</i> از 1</span></span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:112%"><span new="" roman="" style="font-family:" times=""><span style="color:black">&plusmn;</span></span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:112%"><span b="" nazanin="" style="font-family:"><span style="color:black">9 به 3/05</span></span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:112%"><span new="" roman="" style="font-family:" times=""><span style="color:black">&plusmn;</span></span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:112%"><span b="" nazanin="" style="font-family:"><span style="color:black"> 19/66افزایش داشت </span></span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:112%"><span b="" nazanin="" style="font-family:">(0/01</span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:112%"><span new="" roman="" style="font-family:" times="">P-value =</span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:112%"><span b="" nazanin="" style="font-family:">)</span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:112%"><span new="" roman="" style="font-family:" times=""><span style="color:black"> .</span></span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:112%"><span b="" nazanin="" style="font-family:"><span style="color:black"> میزان </span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:112%"><span new="" roman="" style="font-family:" times=""><span style="color:black">MIC </span></span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:112%"><span b="" nazanin="" style="font-family:"><span style="color:black">&nbsp;و </span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:112%"><span new="" roman="" style="font-family:" times=""><span style="color:black">MBC</span></span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:112%"><span b="" nazanin="" style="font-family:"><span style="color:black"> برای نانوذره روی به ترتیب 0/15 و 0/24 میلی‌گرم بر میلی‌لیتر&nbsp; بود. در حالی که میزان </span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:112%"><span new="" roman="" style="font-family:" times=""><span style="color:black">MIC </span></span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:112%"><span b="" nazanin="" style="font-family:"><span style="color:black">&nbsp;و </span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:112%"><span new="" roman="" style="font-family:" times=""><span style="color:black">MBC</span></span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:112%"><span b="" nazanin="" style="font-family:"><span style="color:black"> برای نانوذره روی</span></span></span></span> <span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:112%"><span b="" nazanin="" style="font-family:"><span style="color:black">+ حلال بنزآلکونیوم کلراید 10 درصد به ترتیب 0/03 و 0/04 بود.</span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:112%"><span style="font-family:"Times New Roman",serif"></span></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:112%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><span lang="AR-SA" style="line-height:112%"><span b="" nazanin="" style="font-family:">نتیجه</span></span></b><b><span dir="LTR" style="font-size:10.0pt"><span style="line-height:112%">‌</span></span></b><b><span lang="AR-SA" style="line-height:112%"><span b="" nazanin="" style="font-family:">گیری</span></span></b><span lang="AR-SA" style="line-height:112%"><span b="" nazanin="" style="font-family:">:</span></span> <span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:112%"><span b="" nazanin="" style="font-family:"><span style="color:black">به نظر می‌رسد که استوک نانو ذرات&shy; با حلال بنزآلکونیوم کلراید در مقابل باکتری <i>‌سودوموناس آئروژینوزا</i> در غلظت&shy;های مختلف دارای اثر</span></span></span></span>&nbsp; <span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:112%"><span b="" nazanin="" style="font-family:"><span style="color:black">&nbsp;باکتری&shy; کشی یا ممانعت‌کنندگی رشد است و در غلظت&shy; های بیشتر از غلظت</span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:112%"><span new="" roman="" style="font-family:" times=""><span style="color:black"> MIC </span></span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:112%"><span b="" nazanin="" style="font-family:"><span style="color:black">&nbsp;دارای اثر باکتری&shy; کشی سریع است. از</span></span></span></span> <span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:112%"><span b="" nazanin="" style="font-family:"><span style="color:black">نانوذره</span></span></span></span> <span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:112%"><span b="" nazanin="" style="font-family:"><span style="color:black">اکسید</span></span></span></span> <span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:112%"><span b="" nazanin="" style="font-family:"><span style="color:black">روی</span></span></span></span> <span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:112%"><span b="" nazanin="" style="font-family:"><span style="color:black">می‌توان</span></span></span></span> <span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:112%"><span b="" nazanin="" style="font-family:"><span style="color:black">برای</span></span></span></span> <span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:112%"><span b="" nazanin="" style="font-family:"><span style="color:black">پیشگیری</span></span></span></span> <span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:112%"><span b="" nazanin="" style="font-family:"><span style="color:black">و</span></span></span></span> <span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:112%"><span b="" nazanin="" style="font-family:"><span style="color:black">یا</span></span></span></span> <span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:112%"><span b="" nazanin="" style="font-family:"><span style="color:black">کمک</span></span></span></span> <span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:112%"><span b="" nazanin="" style="font-family:"><span style="color:black">به</span></span></span></span> <span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:112%"><span b="" nazanin="" style="font-family:"><span style="color:black">درمان</span></span></span></span> <span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:112%"><span b="" nazanin="" style="font-family:"><span style="color:black">عفونت‌های</span></span></span></span> <span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:112%"><span b="" nazanin="" style="font-family:"><span style="color:black">سودوموناس</span></span></span></span> <span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:112%"><span b="" nazanin="" style="font-family:"><span style="color:black">آئروجینوزا</span></span></span></span> <span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:112%"><span b="" nazanin="" style="font-family:"><span style="color:black">استفاده</span></span></span></span> <span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:112%"><span b="" nazanin="" style="font-family:"><span style="color:black">کرد.</span></span></span></span><span lang="AR-SA" style="line-height:112%"><span style="font-family:"B Nazanin""></span></span></span></span></span></span></span></div> <div style="text-align: justify;"><span style="font-size:11pt"><span style="line-height:112%"><span style="font-family:Calibri,sans-serif"><b><span style="font-size:10.0pt"><span style="line-height:112%"><span new="" roman="" style="font-family:" times="">Background and Aim:</span></span></span></b> <span style="font-size:10.0pt"><span style="line-height:112%"><span new="" roman="" style="font-family:" times=""><span style="color:black">Nowadays, the use of metals such as silver, copper, gold, and aluminum in various forms including nanoparticles as antimicrobial and bactericidal compounds has become popular. The present study aimed to evaluate the effect of zinc nanoparticles on <i>‌‌‌‌Pseudomonas aeruginosa </i>(ATCC 27853).</span></span></span></span><span style="font-size:10.0pt"><span style="background:white"><span style="line-height:112%"><span style="font-family:"Times New Roman",serif"></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:112%"><span style="font-family:Calibri,sans-serif"><b><span style="font-size:10.0pt"><span style="background:white"><span style="line-height:112%"><span new="" roman="" style="font-family:" times="">Methods:</span></span></span></span></b> <span style="font-size:10.0pt"><span style="line-height:112%"><span new="" roman="" style="font-family:" times=""><span style="color:black">In the current experimental study, zinc nanoparticles prepared from Nano Pars Co. <i>‌‌‌‌Pseudomonas aeruginosa </i>ATCC 27853 was used as the indicator microorganism. The antibacterial activity of nano-particles was compared with benzalkonium using agar diffusion (disk diffusion) and dilution methods.</span></span></span></span><span style="font-size:10.0pt"><span style="line-height:112%"><span new="" roman="" style="font-family:" times=""> <span style="color:black">Data were statistically analyzed using SPSS software, version 22 (IBM, Armonk, NY, USA) running one way ANOVA and Tukey test.</span><span style="background:white"></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:112%"><span style="font-family:Calibri,sans-serif"><b><span style="font-size:10.0pt"><span style="background:white"><span style="line-height:112%"><span new="" roman="" style="font-family:" times="">Results:</span></span></span></span></b> <span style="font-size:10.0pt"><span style="line-height:112%"><span new="" roman="" style="font-family:" times=""><span style="color:black">The results showed that the addition of zinc nanoparticles to the benzalkonium chloride solvent increased the diameter of the inhibitory zone of <i>Pseudomonas</i> spp. from 9&plusmn;1 to 19.66 &plusmn; 3.05 )</span></span></span></span><span style="font-size:10.0pt"><span style="line-height:112%"><span new="" roman="" style="font-family:" times="">P-value =0.001)<span style="color:black">. The MIC and MBC for zinc nanoparticles were 0.15 and 0.24 mg/ml, respectively, while the MIC and MBC for nanoparticles of zinc+benzalconium chloride 10% were 0.03 and 0.04, respectively. This indicates the excellent antibacterial activity of nanoparticles in combination with benzalkonium chloride solvent against <i>Pseudomonas</i> spp.</span><span style="background:white"></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:112%"><span style="font-family:Calibri,sans-serif"><b><span style="font-size:10.0pt"><span style="line-height:112%"><span new="" roman="" style="font-family:" times="">Conclusion:</span></span></span></b> <span style="font-size:10.0pt"><span style="line-height:112%"><span new="" roman="" style="font-family:" times=""><span style="color:black">It seems that the stock of nanoparticles with benzalkonium chloride solvent has bactericidal or growth inhibitory effect against <i>‌‌‌‌Pseudomonas aeruginosa </i>in different concentrations and has rapid bactericidal effect in concentrations higher than MIC concentration.</span></span></span></span><span style="font-size:10.0pt"><span style="line-height:112%"><span new="" roman="" style="font-family:" times=""> <span style="color:black">Zinc oxide nanoparticles can be used to prevent or help treat <i>‌‌‌‌Pseudomonas aeruginosa </i>infections.</span></span></span></span><span style="font-size:10.0pt"><span style="line-height:112%"><span style="font-family:"Times New Roman",serif"></span></span></span></span></span></span></div> نانو ذره اکسید روی؛ ‌سودوموناس آئروژینوزا؛ MIC؛ انتشار در آگار Zinc oxide nanoparticles, Pseudomonas aeruginosa, MIC, Agar diffusion 41 49 http://pejouhesh.sbmu.ac.ir/browse.php?a_code=A-10-4357-1&slc_lang=fa&sid=1 Milad Mohammadyari میلاد محمدیاری 100319475328460027632 100319475328460027632 No Department of Biology, Sanandaj Branch, Islamic Azad University, Sanandaj, Iran. گروه زیست‌شناسی، واحد سنندج، دانشگاه آزاد اسلامی، سنندج، ایران. ziba Mozaffari زیبا مظفری Mozaffari_Ziba882@yahoo.com 100319475328460027633 100319475328460027633 Yes Department of Biology, Sanandaj Branch, Islamic Azad University, Sanandaj, Iran. گروه زیست‌شناسی، واحد سنندج، دانشگاه آزاد اسلامی، سنندج، ایران. Bahareh Rahimian Zarif بهاره رحیمیان ظریف 100319475328460027634 100319475328460027634 No Department of Biology, Sanandaj Branch, Islamic Azad University, Sanandaj, Iran. گروه زیست‌شناسی، واحد سنندج، دانشگاه آزاد اسلامی، سنندج، ایران.