Pejouhesh dar Pezeshki (Research in Medicine)
پژوهش در پزشکی
Research in Medicine
Medical Sciences
http://pejouhesh.sbmu.ac.ir
1
journal1
1735-5311
2008-0506
fa
jalali
1401
6
1
gregorian
2022
9
1
46
3
online
1
fulltext
fa
اثر تزریق سلول های فیبروبلاست برالتیام زخم های دیابتی و بهبود کشسانی و ضخامت پوست در موشهای صحرایی نر
Effect of Fibroblast Cells Injection on Healing of Diabetic Wounds and Improvement of Skin Elasticity and Thickness in Male Rats
زیست شناسی
Biology
پژوهشی
Original
<div style="text-align: justify;"><span style="font-size:11pt"><span style="line-height:110%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><span lang="AR-SA" style="line-height:110%"><span style="font-family:"B Nazanin"">سابقه و هدف</span></span></b><b><span lang="AR-SA" style="font-family:"B Nazanin"">: </span></b><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"B Nazanin"">سلولهای بنیادی در التیام زخمها نقش مؤثری دارند، گرچه مکانیسم اثرات ترمیمی این سلول</span></span></span><span lang="FA" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"B Nazanin""></span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"B Nazanin"">ها هنوز هم روشن نیست. مطالعه حاضر به بررسی اثر تزریق سلولهای فیبروبلاست بر التیام زخمهای دیابتی و تغییرات ضخامت و کشسانی پوست در موشهای صحرایی نر پرداخته است</span></span></span><span lang="FA" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"B Nazanin"">. این تحقیق </span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"B Nazanin""> طی سال 1399 در آزمایشگاه بیوتکنولوژی جاوید انجام گرفت.</span></span></span><span lang="AR-SA" style="line-height:110%"><span style="font-family:"B Nazanin""></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:110%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><span lang="AR-SA" style="line-height:110%"><span style="font-family:"B Nazanin"">روش کار:</span></span></b> <span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"B Nazanin"">طی این تحقیق تجربی-آزمایشگاهی</span></span></span> <span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"B Nazanin"">سلولهای</span></span></span> <span lang="FA" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"B Nazanin"">فیبروبلاست از پوست ختنهگاه جداسازی شدند. 12 سر موش­ صحرایی نر نژاد ویستار دیابتی شده با استرپتوزوتوسین به دو گروه کنترل (دریافت کننده نرمالسالین) و تیمار شده با سلول­های فیبروبلاست تقسیمبندی شدند. زخمهایی به قطر </span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"B Nazanin"">8/0</span></span></span> <span lang="FA" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"B Nazanin"">سانتیمتر به وسیله پانچ­ بیوپسی در ناحیه پشت موشها ایجاد شدند</span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"B Nazanin"">. </span></span></span><span lang="FA" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"B Nazanin"">در روز اول تجربه و روزهای 7، 14 و 21 پس از تیمار با استفاده مشاهده مورفولوژیک و سونوگرافی و سنجش الاستیسیته، ترمیم پوست ارزیابی شد. داده­ها با استفاده از آزمون آنالیز واریانس یکطرفه تجزیه و تحلیل شدند.</span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"Times New Roman",serif"></span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:110%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><span lang="AR-SA" style="line-height:110%"><span style="font-family:"B Nazanin"">یافته</span></span></b><b><span dir="LTR" style="font-size:10.0pt"><span style="line-height:110%"></span></span></b><b><span lang="AR-SA" style="line-height:110%"><span style="font-family:"B Nazanin"">ها:</span></span></b> <span lang="FA" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"B Nazanin"">سرعت التیام زخم همراه با میزان ضخامت و کشسانی پوست در گروه تیمار با سلولهای فیبروبلاست نسبت به گروه کنترل افزایش معناداری یافت. طی روزهای 14 و 21 پس از تیمار و در مقایسه با روز 7 افزایش معنادار خاصیت ارتجاعی </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"Times New Roman",serif">(R2)</span></span></span><span lang="FA" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"B Nazanin"">،</span></span></span> <span lang="FA" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"B Nazanin""> و</span></span></span> <span lang="FA" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"B Nazanin"">بهبودی پس از تغییر شکل </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"Times New Roman",serif">R7</span></span></span><span lang="FA" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"B Nazanin""> (به ترتیب 0/01></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"Times New Roman",serif">p</span></span></span> <span lang="FA" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"B Nazanin""> و 0/001></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"Times New Roman",serif">p</span></span></span><span lang="FA" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"B Nazanin"">) و کشسانی خالص </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"Times New Roman",serif">(</span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"Times New Roman",serif">R5</span></span></span><span lang="FA" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"B Nazanin""> (به ترتیب 0/001></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"Times New Roman",serif">p</span></span></span> <span lang="FA" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"B Nazanin"">و 0/01></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"Times New Roman",serif">p</span></span></span><span lang="FA" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"B Nazanin"">) در پوست ناحیه زخم مشاهده شد.</span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"Times New Roman",serif"></span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:110%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><span lang="AR-SA" style="line-height:110%"><span style="font-family:"B Nazanin"">نتیجه</span></span></b><b><span dir="LTR" style="font-size:10.0pt"><span style="line-height:110%"></span></span></b><b><span lang="AR-SA" style="line-height:110%"><span style="font-family:"B Nazanin"">گیری</span></span></b><span lang="AR-SA" style="line-height:110%"><span style="font-family:"B Nazanin"">:</span></span> <span lang="FA" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"B Nazanin"">به نظر میرسد که سلول­ های فیبروبلاست مشتق شده از پوست ختنه­گاه می­توانند از طریق افزایش ضخامت و کشسانی پوست سبب تسریع التیام زخم دیابتی شوند.</span></span></span><span lang="AR-SA" style="line-height:110%"><span style="font-family:"B Nazanin""></span></span></span></span></span></span></span></div>
<div style="text-align: justify;"><span style="font-size:11pt"><span style="line-height:112%"><span style="font-family:Calibri,sans-serif"><b><span style="font-size:10.0pt"><span style="line-height:112%"><span style="font-family:"Times New Roman",serif">Background and Aim:</span></span></span></b> <span lang="EN" style="font-size:10.0pt"><span style="line-height:112%"><span style="font-family:"Times New Roman",serif">Stem cells have a pivotal role in wounds healing; however, the </span></span></span><span style="font-size:10.0pt"><span style="line-height:112%"><span style="font-family:"Times New Roman",serif">mechanism of the healing effect of these cells is not still clear</span></span></span><span lang="EN" style="font-size:10.0pt"><span style="line-height:112%"><span style="font-family:"Times New Roman",serif">. The present study was carried out to investigate the effect of fibroblast cells on diabetic wound healing and skin thickness and elasticity in male rats. This research</span></span></span> <span lang="EN" style="font-size:10.0pt"><span style="line-height:112%"><span style="font-family:"Times New Roman",serif">was done in Javid Biotechnology Laboratory in 2021.</span></span></span><span style="font-size:10.0pt"><span style="background:white"><span style="line-height:112%"><span style="font-family:"Times New Roman",serif"></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:112%"><span style="font-family:Calibri,sans-serif"><b><span style="font-size:10.0pt"><span style="background:white"><span style="line-height:112%"><span style="font-family:"Times New Roman",serif">Methods:</span></span></span></span></b> <span lang="EN" style="font-size:10.0pt"><span style="line-height:112%"><span style="font-family:"Times New Roman",serif">In the present experimental laboratory study,</span></span></span> <span lang="EN" style="font-size:10.0pt"><span style="line-height:112%"><span style="font-family:"Times New Roman",serif">fibroblast cells were isolated from the foreskin. Totally, 12 streptozotocin-induced diabetic male Wistar rats were divided into control (normal saline-treated) and fibroblast-treated rats. Wounds with a diameter of 0.8 cm were created via a biopsy puncture in the back of the mice. On days 7, 14, and 21 after treatment, the wound healing was evaluated via morphological observation, sonography, and elasticity assessment. Data were analyzed using one-way analysis of variance.</span></span></span><span style="font-size:10.0pt"><span style="background:white"><span style="line-height:112%"><span style="font-family:"Times New Roman",serif"></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:112%"><span style="font-family:Calibri,sans-serif"><b><span style="font-size:10.0pt"><span style="background:white"><span style="line-height:112%"><span style="font-family:"Times New Roman",serif">Results:</span></span></span></span></b> <span lang="EN" style="font-size:10.0pt"><span style="line-height:112%"><span style="font-family:"Times New Roman",serif">The healing</span></span></span><span style="font-size:10.0pt"><span style="line-height:112%"><span style="font-family:"Times New Roman",serif"> rate and skin thickness and elasticity </span></span></span><span lang="EN" style="font-size:10.0pt"><span style="line-height:112%"><span style="font-family:"Times New Roman",serif">significantly increased in fibroblast cells treated group </span></span></span><span style="font-size:10.0pt"><span style="line-height:112%"><span style="font-family:"Times New Roman",serif">compared with control group</span></span></span><span lang="EN" style="font-size:10.0pt"><span style="line-height:112%"><span style="font-family:"Times New Roman",serif">.</span></span></span><span style="font-size:10.0pt"><span style="line-height:112%"><span style="font-family:"Times New Roman",serif"> Compared with day 7, on days 14 and 21 after treatment, we observed significant increase in wound area skin gross elasticity (R2), recovery after deformation (R7) </span></span></span><span style="font-size:10.0pt"><span style="line-height:112%"><span style="font-family:"Times New Roman",serif">(p=0.008<0.01 and p=0.0007<0.001, respectively), and net elasticity (R5) </span></span></span><span style="font-size:10.0pt"><span style="line-height:112%"><span style="font-family:"Times New Roman",serif">(p=0.0008<0.001and p=0.009<0.01, respectively).</span></span></span><span style="font-size:10.0pt"><span style="background:white"><span style="line-height:112%"><span style="font-family:"Times New Roman",serif"></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:112%"><span style="font-family:Calibri,sans-serif"><b><span style="font-size:10.0pt"><span style="line-height:112%"><span style="font-family:"Times New Roman",serif">Conclusion:</span></span></span></b> <span style="font-size:10.0pt"><span style="line-height:112%"><span style="font-family:"Times New Roman",serif">The results of the present study showed that foreskin derived fibroblast cells can accelerate diabetic wound healing by increasing the skin thickness and elasticity.</span></span></span><span style="font-size:10.0pt"><span style="line-height:112%"><span style="font-family:"Times New Roman",serif"></span></span></span></span></span></span><br>
<span dir="RTL"></span></div>
زخم دیابتی؛ فیبروبلاست؛ ضخامت پوست؛ الاستیسیته؛ موش صحرایی
Diabetic wound, Fibroblast, Skin thickness, Elasticity, Rat
105
114
http://pejouhesh.sbmu.ac.ir/browse.php?a_code=A-10-4394-1&slc_lang=fa&sid=1
Abbas
Zabihi
عباس
ذبیحی
100319475328460027650
100319475328460027650
No
Department of Biology, Hamadan Branch, Islamic Azad University, Hamadan, Iran.
گروه زیستشناسی، واحد همدان، دانشگاه آزاد اسلامی، همدان، ایران.
Minoo
Mahmoodi
مینو
محمودی
minoomahmoodi@yahoo.com
100319475328460027651
100319475328460027651
Yes
Department of Biology, Hamadan Branch, Islamic Azad University, Hamadan, Iran.
گروه زیستشناسی، واحد همدان، دانشگاه آزاد اسلامی، همدان، ایران.
Sanaz
Soltani
ساناز
سلطانی
100319475328460027652
100319475328460027652
No
Department of Biology, Shahr-e-Quds Branch, Islamic Azad University, Tehran, Iran.
گروه زیستشناسی، واحد شهرقدس، دانشگاه آزاد اسلامی، تهران، ایران.