Pejouhesh dar Pezeshki (Research in Medicine)
پژوهش در پزشکی
Research in Medicine
Medical Sciences
http://pejouhesh.sbmu.ac.ir
1
journal1
1735-5311
2008-0506
fa
jalali
1401
6
1
gregorian
2022
9
1
46
3
online
1
fulltext
fa
بررسی اثرات نانوذره ی کورکومین بر روی بیان ژنهای دخیل در تولید بیوفیلم در سودوموناس آئروژینوزا
Effect of Curcumin Nanoparticles on Biofilm Gene Expression in Pseudomonas Aeruginosa
میکروبشناسی
Microbiology
پژوهشی
Original
<div style="text-align: justify;"><span style="font-size:11pt"><span style="line-height:110%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><span lang="AR-SA" style="line-height:110%"><span style="font-family:"B Nazanin"">سابقه و هدف</span></span></b><b><span lang="AR-SA" style="font-family:"B Nazanin"">: </span></b><i><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"B Nazanin""><span style="color:black">سودوموناس آئروژینوزا</span></span></span></span></i><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"B Nazanin""><span style="color:black"> به عنوان یکی از مهم</span></span></span></span><span lang="FA" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"B Nazanin""><span style="color:black"></span></span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"B Nazanin""><span style="color:black">ترین عوامل بیماری</span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"Courier New""><span style="color:black"></span></span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"B Nazanin""><span style="color:black">زای بیمارستانی مطرح است. یکی</span></span></span></span> <span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"B Nazanin""><span style="color:black">از</span></span></span></span> <span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"B Nazanin""><span style="color:black">مشکلات</span></span></span></span> <span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"B Nazanin""><span style="color:black">درمانی</span></span></span></span> <span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"B Nazanin""><span style="color:black">این</span></span></span></span> <span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"B Nazanin""><span style="color:black">باکتری، مقاومت آنتیبیوتیکی</span></span></span></span> <span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"B Nazanin""><span style="color:black">آن</span></span></span></span> <span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"B Nazanin""><span style="color:black">به</span></span></span></span> <span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"B Nazanin""><span style="color:black">درمان</span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"Courier New""><span style="color:black"></span></span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"B Nazanin""><span style="color:black">های</span></span></span></span> <span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"B Nazanin""><span style="color:black">رایج آنتیبیوتیکی است که</span></span></span></span> <span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"B Nazanin""><span style="color:black">با</span></span></span></span> <span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"B Nazanin""><span style="color:black">تولید</span></span></span></span> <span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"B Nazanin""><span style="color:black">بیوفیلم</span></span></span></span> <span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"B Nazanin""><span style="color:black">مرتبط</span></span></span></span> <span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"B Nazanin""><span style="color:black">است. مطالعه حاضر با هدف بررسی اثر نانوذره</span></span></span></span> <span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"B Nazanin""><span style="color:black">کورکومین بر روی بیان ژن بیوفیلم در <i>سودوموناس آئروژینوزا</i> در شرایط آزمایشگاهی انجام شده است.</span></span></span></span><span lang="AR-SA" style="line-height:110%"><span style="font-family:"B Nazanin""></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:110%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><span lang="AR-SA" style="line-height:110%"><span style="font-family:"B Nazanin"">روش کار:</span></span></b> <span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"B Nazanin""><span style="color:black">در مطالعه تجربی </span></span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"B Nazanin""><span style="color:black">(</span></span></span></span><i><span dir="LTR" style="font-size:10.0pt"><span style="background:white"><span style="line-height:110%"><span style="font-family:"Times New Roman",serif"><span style="color:black">in vitro</span></span></span></span></span></i><span lang="AR-SA" style="font-size:10.0pt"><span style="background:white"><span style="line-height:110%"><span style="font-family:"B Nazanin""><span style="color:black">)</span></span></span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"B Nazanin""><span style="color:black"> حاضر تعداد </span></span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"B Nazanin""><span style="color:black">50</span></span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"B Nazanin""><span style="color:black"> جدایه <i>سودوموناس آئروژینوزا</i> از بیمارستان فیروزگر جمعآوری شد</span></span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"B Nazanin""><span style="color:black">.</span></span></span></span> <span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"B Nazanin""><span style="color:black">شیوع ژنهای </span></span></span></span><i><span dir="LTR" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"Times New Roman",serif"><span style="color:black">pil</span></span></span></span></i><span dir="LTR" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"Times New Roman",serif"><span style="color:black">A</span></span></span></span><b><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"B Nazanin""><span style="color:black">، </span></span></span></span></b><i><span dir="LTR" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"Times New Roman",serif"><span style="color:black">alg</span></span></span></span></i><span dir="LTR" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"Times New Roman",serif"><span style="color:black">X</span></span></span></span><b><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"B Nazanin""><span style="color:black">، </span></span></span></span></b><i><span dir="LTR" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"Times New Roman",serif"><span style="color:black">las</span></span></span></span></i><span dir="LTR" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"Times New Roman",serif"><span style="color:black">I</span></span></span></span><i> </i><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"B Nazanin""><span style="color:black">و </span></span></span></span><i><span dir="LTR" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"Times New Roman",serif"><span style="color:black">est</span></span></span></span></i><span dir="LTR" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"Times New Roman",serif"><span style="color:black">A</span></span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"B Nazanin""><span style="color:black"> با استفاده از </span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"Times New Roman",serif"><span style="color:black">PCR</span></span></span></span><span lang="FA" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"B Nazanin""><span style="color:black">، </span></span></span></span> <span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"B Nazanin""><span style="color:black">درت</span></span></span></span> <span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"B Nazanin""><span style="color:black">تشکیل بیوفیلم با روش میکروتیتر پلیت و</span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"Times New Roman",serif"><span style="color:black">MIC </span></span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"B Nazanin""><span style="color:black"> برای نانوپارتیکل کورکومین با استفاده از روش </span></span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"B Nazanin""><span style="color:black">میکرودایلوشن براث </span></span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"B Nazanin""><span style="color:black">تعیین شد.</span></span></span></span> <span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"B Nazanin""><span style="color:black">به منظور بررسی تاثیر بازدارندگی نانوپارتیکل کورکومین بر بیان ژنهای مورد بررسی، از تکنیک مولکولی<i> </i></span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"Times New Roman",serif"><span style="color:black">Real-time PCR</span></span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"B Nazanin""><span style="color:black"> استفاده شد. در نهایت دادهها با استفاده از نرمافزارهای آماری </span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"Times New Roman",serif"><span style="color:black">REST 2009</span></span></span></span><span style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"B Nazanin""><span style="color:black"> و</span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"Times New Roman",serif"><span style="color:black">GraphPad Prism 8</span></span></span></span> <span dir="LTR" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"Times New Roman",serif"><span style="color:black"> (GraphPad Software, Inc)</span></span></span></span><span style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"B Nazanin""><span style="color:black"> تجزیه و تحلیل شدند.</span></span></span></span><b> </b><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"B Nazanin""><span style="color:black">آزمون ناپارامتری کروسکال والیس (</span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"Times New Roman",serif"><span style="color:black">Kruskal-Wallis Test</span></span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"B Nazanin""><span style="color:black">) برای بررسی میانگین تغییرات بیان ژنهای مورد مطالعه نسبت به نمونه کنترل در حضور ژن کنترل داخلی </span></span></span></span><i><span dir="LTR" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"Times New Roman",serif"><span style="color:black">16srRNA</span></span></span></span></i><i> </i><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"B Nazanin""><span style="color:black">استفاده شد و</span></span></span></span> <span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"B Nazanin""><span style="color:black">(0/05</span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"Times New Roman",serif"><span style="color:black">P-value<</span></span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"B Nazanin""><span style="color:black">). معنادار در نظر گرفته شد. </span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"Times New Roman",serif"></span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:110%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><span lang="AR-SA" style="line-height:110%"><span style="font-family:"B Nazanin"">یافته</span></span></b><b><span dir="LTR" style="font-size:10.0pt"><span style="line-height:110%"></span></span></b><b><span lang="AR-SA" style="line-height:110%"><span style="font-family:"B Nazanin"">ها:</span></span></b> <span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"B Nazanin""><span style="color:black">شیوع ژنهای </span></span></span></span><i><span dir="LTR" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"Times New Roman",serif"><span style="color:black">pil</span></span></span></span></i><span dir="LTR" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"Times New Roman",serif"><span style="color:black">A</span></span></span></span><b><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"B Nazanin""><span style="color:black">، </span></span></span></span></b><i><span dir="LTR" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"Times New Roman",serif"><span style="color:black">alg</span></span></span></span></i><span dir="LTR" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"Times New Roman",serif"><span style="color:black">X</span></span></span></span><b><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"B Nazanin""><span style="color:black">، <i> </i></span></span></span></span></b><i><span dir="LTR" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"Times New Roman",serif"><span style="color:black">las</span></span></span></span></i><span dir="LTR" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"Times New Roman",serif"><span style="color:black">I</span></span></span></span><i> </i><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"B Nazanin""><span style="color:black"> و </span></span></span></span><i><span dir="LTR" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"Times New Roman",serif"><span style="color:black">est</span></span></span></span></i><span dir="LTR" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"Times New Roman",serif"><span style="color:black">A</span></span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"B Nazanin""><span style="color:black"> به ترتیب 40 درصد، 100درصد، 100</span></span></span></span><span lang="FA" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"B Nazanin""><span style="color:black">درصد</span></span></span></span> <span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"B Nazanin""><span style="color:black">و 94 درصد بود. 38 درصد سویهها </span></span></span></span><span lang="FA" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"B Nazanin""><span style="color:black">تولیدکننده</span></span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"B Nazanin""><span style="color:black"> بیوفیلم قوی، 54 درصد متوسط و 8درصد ضعیف بودند. </span></span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"B Nazanin""><span style="color:black">مقدار</span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"Times New Roman",serif"><span style="color:black">MIC </span></span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"B Nazanin""><span style="color:black"> برای همه جدایهها و </span></span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"B Nazanin""><span style="color:black">سویه کنترل مثبت </span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"Times New Roman",serif"><span style="color:black">PAO1</span></span></span></span><span style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"B Nazanin""><span style="color:black"> یکسان و غلظتی معادل 128 میکروگرم در میلیلیتر از نانوذره بود. </span></span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"B Nazanin""><span style="color:black">میزان بیان ژنهای </span></span></span></span><i><span dir="LTR" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"Times New Roman",serif"><span style="color:black">pil</span></span></span></span></i><span dir="LTR" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"Times New Roman",serif"><span style="color:black">A</span></span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"B Nazanin""><span style="color:black">، <i> </i></span></span></span></span><i><span dir="LTR" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"Times New Roman",serif"><span style="color:black">alg</span></span></span></span></i><span dir="LTR" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"Times New Roman",serif"><span style="color:black">X</span></span></span></span><i> </i><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"B Nazanin""><span style="color:black">و<i> </i></span></span></span></span><i><span dir="LTR" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"Times New Roman",serif"><span style="color:black">las</span></span></span></span></i><span dir="LTR" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"Times New Roman",serif"><span style="color:black">I</span></span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"B Nazanin""><span style="color:black"> به ترتیب 1/91، 2/9 و<i> </i>3/98<i> </i></span></span></span></span><span lang="FA" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"B Nazanin""><span style="color:black">بار</span></span></span></span> <span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"B Nazanin""><span style="color:black">کاهش یافت. اما میزان بیان ژن </span></span></span></span><i><span dir="LTR" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"Times New Roman",serif"><span style="color:black">est</span></span></span></span></i><span dir="LTR" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"Times New Roman",serif"><span style="color:black">A</span></span></span></span><b><i> </i></b><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"B Nazanin""><span style="color:black">0/19 افزایش نشان داد</span></span></span></span><span lang="FA" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"B Nazanin""><span style="color:black">. تغییرات مشاهده شده در بیان ژن از لحاظ آماری معنادار بود</span></span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"B Nazanin""><span style="color:black"> (0/</span></span></span></span><span lang="FA" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"B Nazanin""><span style="color:black">05</span></span></span></span> <span dir="LTR" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"Times New Roman",serif"><span style="color:black"><</span></span></span></span> <span dir="LTR" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"Times New Roman",serif"><span style="color:black">P-value</span></span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"B Nazanin""><span style="color:black">).</span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"Times New Roman",serif"></span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:110%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><span lang="AR-SA" style="line-height:110%"><span style="font-family:"B Nazanin"">نتیجه</span></span></b><b><span dir="LTR" style="font-size:10.0pt"><span style="line-height:110%"></span></span></b><b><span lang="AR-SA" style="line-height:110%"><span style="font-family:"B Nazanin"">گیری</span></span></b><span lang="AR-SA" style="line-height:110%"><span style="font-family:"B Nazanin"">:</span></span> <span lang="AR-SA" style="font-size:10.0pt"><span style="background:white"><span style="line-height:110%"><span style="font-family:"B Nazanin""><span style="color:black">با توجه به تاثیر مهاری نانوکورکومین بر ژن بیوفیلم <i>سودوموناس آئروژینوزا</i> احتمالا بتوان از این نانوذره به عنوان یک گزینه درمانی علیه این باکتری و مهار ژن ویرولانس آن استفاده کرد</span></span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="background:white"><span style="line-height:110%"><span style="font-family:"Times New Roman",serif"><span style="color:black">.</span></span></span></span></span><span lang="AR-SA" style="line-height:110%"><span style="font-family:"B Nazanin""></span></span></span></span></span></span></span></div>
<div style="text-align: justify;"><span style="font-size:11pt"><span style="line-height:110%"><span style="font-family:Calibri,sans-serif"><b><span style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"Times New Roman",serif">Background and Aim:</span></span></span></b> <i><span style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"Times New Roman",serif"><span style="color:black">Pseudomonas aeruginosa </span></span></span></span></i><span style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"Times New Roman",serif"><span style="color:black">is one of the most important nosocomial pathogens. One of the therapeutic problems of this bacterium is antibiotic resistance, which is associated with biofilm production. The aim of the present study was to investigate the effect of curcumin nanoparticles on biofilm gene expression in <i>Pseudomonas aeruginosain vitro</i>.</span></span></span></span><span style="font-size:10.0pt"><span style="background:white"><span style="line-height:110%"><span style="font-family:"Times New Roman",serif"></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:110%"><span style="font-family:Calibri,sans-serif"><b><span style="font-size:10.0pt"><span style="background:white"><span style="line-height:110%"><span style="font-family:"Times New Roman",serif">Methods:</span></span></span></span></b> <span style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"Times New Roman",serif"><span style="color:black">In the present <i>in vitro</i> study, 50 <i>Pseudomonas aeruginosa</i>isolates were collected from Firoozgar Hospital. The prevalence of <i>pil</i>A, <i>alg</i>X, <i>las</i>I, and <i>est</i>A genes was determined using PCR, biofilm formation ability using microtiter plate, and MIC for curcumin nanoparticles using microdilution broth method. Also, in order to investigate the inhibition effect of curcumin nanoparticle on gene expression, Real-time PCR was run. Data were analyzed using REST 2009 and GraphPad Prism 8 (GraphPad Software, Inc.). The non-parametric Kruskal-Wallis test was used to evaluate the mean changes in the expression of the studied genes compared to the control sample in the presence of 16srRNA internal control gene. P-value <0.05 was considered as significant.</span></span></span></span><span style="font-size:10.0pt"><span style="background:white"><span style="line-height:110%"><span style="font-family:"Times New Roman",serif"></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:110%"><span style="font-family:Calibri,sans-serif"><b><span style="font-size:10.0pt"><span style="background:white"><span style="line-height:110%"><span style="font-family:"Times New Roman",serif">Results:</span></span></span></span></b> <span style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"Times New Roman",serif"><span style="color:black">The prevalence of <i>pil</i>A, <i>alg</i>X, <i>las</i>I and <i>est</i>A genes were 40%, 100%, 100%, and 94%, respectively. In addition, 38% of strains formed strong biofilm, 54% moderate, and 8% weak. The MIC values for all strains and PAO1 positive control strains were the same with the concentration of 128 μg / ml of nanoparticles.</span></span></span></span> <span style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"Times New Roman",serif"><span style="color:black">The expression levels of <i>pil</i>A, <i>alg</i>X, and <i>las</i>I decreased by 1.91, 2.9, and 3.98 folds, respectively, but <i>est</i>A gene expression increased by 0.19</span></span></span></span><span dir="RTL" lang="AR-SA" style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"B Mitra""><span style="color:black">.</span></span></span></span><span style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"Times New Roman",serif"><span style="color:black"> These observed changes in gene expression were found to be statistically significant (P-value <.05).</span></span></span></span><span style="font-size:10.0pt"><span style="background:white"><span style="line-height:110%"><span style="font-family:"Times New Roman",serif"></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:110%"><span style="font-family:Calibri,sans-serif"><b><span style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"Times New Roman",serif">Conclusion:</span></span></span></b> <span style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"Times New Roman",serif"><span style="color:black">Due to the inhibitory effect of nanocurcumin on genes involved in the formation of <i>Pseudomonas aeruginosa</i>biofilm, it is possible that this nanoparticle could be used as a therapeutic option against this bacterium and to inhibit the genes involved in its virulence.</span></span></span></span><span style="font-size:10.0pt"><span style="line-height:110%"><span style="font-family:"Times New Roman",serif"></span></span></span></span></span></span><br>
<span dir="RTL"></span><span dir="RTL"></span></div>
سودوموناس آئروژینوزا؛ بیوفیلم؛ نانوکورکومین؛ ویرولانس؛ Real-time PCR
Pseudomonas aeruginosa, Biofilm, Nanocurcumin, virulence, Real-time PCR
95
104
http://pejouhesh.sbmu.ac.ir/browse.php?a_code=A-10-4430-1&slc_lang=fa&sid=1
Reyhaneh
Dariushy
ریحانه
داریوشی
100319475328460027648
100319475328460027648
No
Department of Microbiology, Faculty of Basic Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran.
گروه تخصصی میکروبیولوژی، دانشکده علوم پایه، دانشگاه آزاد اسلامی، واحد علوم تحقیقات، تهران، ایران.
Fatemeh
Ashrafi
فاطمه
اشرفی
mnfa.ashrafi@yahoo.com
100319475328460027649
100319475328460027649
Yes
Department of Microbiology, Faculty of Basic Sciences, Islamic Azad University, North Tehran Branch, Tehran, Iran.
گروه تخصصی میکروبیولوژی، دانشکده علوم پایه، دانشگاه آزاد اسلامی، واحد تهران شمال، تهران، ایران.