School of Medicine, Shahid Beheshti University of Medical Sciences Research in Medicine 1735-5311 48 4 2025 2 1 The Study of Changes in the Expression of Bax and Bcl-2 Genes in MCF-7 Cell Line Treated with Selenium Nanoparticles Containing Anthemis Atropatana Extract 1 13 FA Mahsa Kavousi Department of Biology, East Tehran Branch, Islamic Azad University, Tehran, Iran. Hadis Toosi Department of Biology, East Tehran Branch, Islamic Azad University, Tehran, Iran Amir Mirzaee Department of Biology, Parand Branch, Islamic Azad University, Parand, Iran Abstract Background and Aim: Studies have shown that changes in the expression level of some genes play a role in the development of cancer. The aim of this study is to investigate the changes in the expression of apoptotic genes Bax and Bcl-2 in MCF-7 cell line after treatment with selenium nanoparticles containing chamomile extract. Methods: This study is a practical and experimental intervention. One- way analysis (ANOVA) and t-test was used for statistical analysis. After synthesizing selenium nanoparticles with Anthemis atropatana extract, the correctness of the synthesis was confirmed by SEM, TEM and EDX. To perform MTT and calculate the IC50, the cells (cancer cell line) MCF-7 and (healthy cell line) MCF-10 were treated with selenium nanoparticles for 24 hours. Finally, the changes in Bax and Bcl-2 gene expression were examined. Results: The results of checking the accuracy of the synthesis of selenium nanoparticles showed that the synthesized nanoparticles were spherical and had a diameter of 33.40 nm. The IC50 concentration was 6 µg/ ml. The results of real- time PCR showed that after 24-hour treatment with the IC50 concentration of selenium nanoparticles, the expression of apoptotic gene Bax increased significantly by 2.69 ± 0.31-fold (P-value < 0.001) and the expression of anti- apoptotic gene Bcl-2 decreased significantly by 0.21 ± 0.17-fold (P-value < 0.001). Conclusion: Selenium nanoparticles containing chamomile extract modulated gene expression in a manner that promotes apoptosis, suggesting their potential as therapeutic agents.

School of Medicine, Shahid Beheshti University of Medical Sciences Research in Medicine 1735-5311 48 4 2025 2 1 The Effect of L-Arginine on Growth of Frozen- Thawed Caprine Spermatogonial Stem Cells in Short- Term Culture 14 25 FA Fatemeh Najafi Department of Clinical Sciences, Faculty of Veterinary Medicine, Razi University, Kermanshah, Iran. Peyman Rahimi-Feyli Department of Clinical Sciences, Faculty of Veterinary Medicine, Razi University, Kermanshah, Iran. Ali Moghaddam Department of Clinical Sciences, Faculty of Veterinary Medicine, Razi University, Kermanshah, Iran. Tayebeh Mohammadi Department of Basic Sciences, Faculty of Veterinary Medicine, Razi University, Kermanshah, Iran. Background and Aim: Developing culture and cryopreservation methods for spermatogonial stem cells (SSCs) is essential for genetic studies, regenerative medicine, transgenic animal production and recombinant drug development. Establishing an optimal culture system for these cells is crucial. This study aimed to investigate the in vitro effects of different concentrations of L-arginine on the proliferation of frozen-thawed caprine SSCs. Methods: This experimental study was repeated five times. Isolated spermatogonial cells were frozen- thawed and cultured in six groups with a basal culture medium (DMEM containing 1% antibiotics and 5% FBS) supplemented with different concentrations of L-arginine (0, 50, 100, 200, 500 and 1000 μmol/L). All experimental groups were cultured for 10 days. The number and surface area of colonies formed were assessed on days 4, 7, and 10. Expression of spermatogonial genes (ID4, BCL6, PGP9.5, VASA, PLZF and OCT4) was determined by RT-PCR. Results: The viability rates of fresh cells before and after the addition of a cryoprotectant agent were 87.14 ± 2.6% and 80.72 ± 1.21%, respectively, which decreased to 65.38 ± 1.26% after thawing. The number and surface area of spermatogonial colonies in the fourth treatment group (200 μmol/L L-arginine) were significantly higher compared to other experimental groups (P < 0.05). Finally, the isolated cells expressed SSC surface markers. Conclusion: The addition of 200 μmol/L L-arginine likely enhances the colonization of frozen-thawed goat SSCs in short- term culture.

School of Medicine, Shahid Beheshti University of Medical Sciences Research in Medicine 1735-5311 48 4 2025 2 1 The Effect of Using Energy Conservation Methods on the Quality of Life and Depression of Patients with Heart Failure 26 38 FA Faranak Kargar Cardiovascular Research Center, Rajaie Cardiovascular Institute, Tehran, Iran. Motahare Nabizade Cardiovascular Research Center, Rajaie Cardiovascular Institute, Tehran, Iran. Marzieh Mirtajaddini Cardiovascular Research Center, Rajaie Cardiovascular Institute, Tehran, Iran. Fidan Shabani Cardiovascular Nursing Research Center, Rajaie Cardiovascular Institute, Tehran, Iran. Mohadese Nabizade Student Research Committee. MSc in Intensive Care Nursing, Department of Nursing, School of Nursing and Midwifery, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Background and Aim: Heart failure is a complex syndrome that leads to a decrease in quality of life. Self- care is a fundamental component of heart failure management. One method of teaching self-care is the use of energy conservation methods. This study aimed to investigate the effect of implementing energy conservation methods on the quality of life and depression in patients with heart failure. Methods: This was a clinical trial study conducted on 100 participants in intervention and control groups, without blinding, and with a block randomized allocation of size 4 in 1403 at Shahid Rajaei Cardiovascular, medical, Research, and Educational Institute, Tehran. Each group consisted of 50 participants. Before the intervention, the demographic tool, the Minnesota tool and the Beck Depression tool were completed by both groups. Energy conservation methods were taught to the intervention group. After 6 weeks, the Minnesota and Beck Depression tools were re- administered to both groups by telephone. For data analysis, SPSS version 24 was used, and the chi-square, Fisher's exact, independent t-test, Mann-Whitney U, and Wilks' lambda tests were employed. Results: After accounting for attrition, 47 individuals in the intervention group and 48 in the control group were analyzed. Based on the questionnaire results, before the intervention, there were no significant differences between the two groups in demographic information and questionnaire scores (P > 0.05), indicating homogeneity between the groups. However, after the intervention, the mean and standard deviation of the quality of life score (49.25 ± 15.08) decreased significantly in the intervention group (P < 0.001), indicating an improvement in quality of life. Additionally, the mean and standard deviation of depression (22.61 ± 5.08) decreased significantly in the intervention group (P < 0.001), suggesting a reduction in depression. In contrast, there was no significant difference in the mean scores of these two questionnaires in the control group (P > 0.001). Conclusion: Energy conservation method is a non-pharmacological method that can increase quality of life and reduce depression in patients with heart failure and can be utilized by patients.

School of Medicine, Shahid Beheshti University of Medical Sciences Research in Medicine 1735-5311 48 4 2025 2 1 Study of Anti- Apoptotic Effects of UIO-66-NH2 Nanoparticles Containing Letrozole on MCF-7 Breast Cancer Cell Line 39 47 FA Fatemeh Gholizadeh Department of Genetics and Biotechnology, School of Biological Science, Varamin- Pishva Branch, Islamic Azad University, Varamin, Iran. Fahimeh Baghbani-Arani Department of Genetics and Biotechnology, School of Biological Science, Varamin- Pishva Branch, Islamic Azad University, Varamin, Iran. Background and Aim: Metal- organic frameworks (MOFs) are groups of hybrid porous materials are constantly being developed due to their porous nature and very high and specific crystalline surfaces for various applications, especially the release of drugs according to specific design. In this research, the anti- apoptotic effect of UIO-66-NH2 nanoparticles containing letrozole on breast cancer cells was investigated. Methods: In this experimental study, after preparing the nano- drug, the structure and morphology of these particles were investigated by FE-SEM method. Then, the toxic effects of the nano- drug were evaluated on the MCF7 cell line by MTT assay. Finally, the expression of Casp3 and Casp9 genes was analyzed by real- time PCR method. GraphPad Prism version 7.01 software was used for statistical calculations. Owing to the regularity of the data distribution, One- Way ANOVA was utilized to compare the treatment and control groups, and p < 0.05 was considered a significant difference. Results: The results showed that the nanoparticles have a polyhedral shape. Also, the UIO-66-NH2-Let component has toxic effects on MCF7 breast cancer cells by a dose- depended manner with the IC50 value of the 1.26 ± 57.71 μg/mL. Also, UIO-66-NH2 nanoparticles containing letrozole at the IC50 concentration were able to significantly increase the expression of the apoptotic genes Casp3 and Casp9 by 4.35 ± 0.62 and 4.70 ± 0.92 times, respectively, compared to the control group (P < 0.001). Conclusion: It seems that the UIO-66-NH2-letrozole has toxic effects on MCF-7 breast cancer. Therefore, it seems useful to use it in studies related to cancer treatment in the future.

School of Medicine, Shahid Beheshti University of Medical Sciences Research in Medicine 1735-5311 48 4 2025 2 1 Analysis of Indexed Scientific Records in the Field of Sports Medicine in the Web of Science Database and Scientific Mapping 48 63 FA jafar Ebadullah Amuqin Department of Knowledge and Information Science, Faculty of Literature and Humanities, University of Qom, Qom, Iran. Behnaz Jafari Kojuri Department of Knowledge and Information Science, Faculty of Literature and Humanities, University of Qom, Qom, Iran. Reyhaneh GHolamisuq Central Library of Shahid Beheshti University of Medical Sciences, Tehran, Iran. Background and Aim: Sports medicine focuses on identifying, treating, and preventing sports- related injuries, playing a crucial role in enhancing athletes' performance and maintaining their health. Research papers authored by influential researchers in this field serve as foundational contributions. Analyzing their work and engaging with these authors can enhance both the quality and quantity of research in sports medicine. Therefore, this study examines scientific records indexed in the Web of Science related to sports medicine and maps the scientific landscape. Methods: This study employs a scientometric approach. Data were extracted from the Web of Science database using the advanced search feature, applying the keywords "sports medicine" and "exercise," yielding 4,481 records. The data were refined and standardized before being analyzed using scientometric software to generate visual maps. The bibliometrix, Netdraw and BibExcel software were utilized for mapping. Results: A scientific field map was generated based on data from the Web of Science database, revealing that the structure of sports medicine research consists of four main clusters. The United States holds the largest share of publications in this field, followed by Australia and the United Kingdom. The keyword "sports medicine" is the most frequently used term, while "orthopedics" and "sports sciences" are the most frequently co-occurring terms, appearing together 681 times. Conclusion: The findings indicate that scientific records in sports medicine have grown significantly from 2019 to 2022. To enhance research impact and collaboration, organizations are encouraged to implement networking policies, increase knowledge exchange, and establish targeted research partnerships.

School of Medicine, Shahid Beheshti University of Medical Sciences Research in Medicine 1735-5311 48 4 2025 2 1 Evaluation of the Role of Staphylococcus Aureus in Angular Cheilitis 64 74 FA Dina Yaghmaie School of Dentistry, International Campus, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. Fatemeh Owlia Department of Oral and Maxillofacial Medicine, School of Dentistry, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. Mohammad-Hasan Akhavan-Karbassi Department of Oral and Maxillofacial Medicine, School of Dentistry, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. Background and Aim: Angular cheilitis is a common condition among dental patients. However, it often responds poorly to antifungal treatment, and the use of corticosteroids can lead to lesion recurrence or medication resistance. This study aimed to assess the frequency of Staphylococcus aureus in patients who visited the Oral and Maxillofacial Medicine Department at Yazd Dental Faculty in 2017. Methods: This case- control study included 163 participants, with 65 patients diagnosed with angular cheilitis in the case group and 98 healthy individuals in the control group. Swabs were collected and inoculated on blood agar and Sabouraud dextrose agar. The cultures were then examined for the presence of Staphylococcus aureus and Candida albicans. Data were analyzed using SPSS software, employing Chi- square and t-tests, with a significance level set at  p < 0.05. Results: The study included 98 healthy individuals and 65 patients with angular cheilitis. The results indicated that Staphylococcus aureus was not detected in either the patient or control group (p < 0.9). A higher prevalence of angular cheilitis was observed among female patients. Additionally, there was a direct correlation between the presence of angular cheilitis and Candida albicans. Conclusion: The findings suggest that Staphylococcus aureus is not a contributing factor in angular cheilitis lesions. Therefore, antibiotic treatment for refractory cases should be postponed until microbial cultures confirm bacterial involvement.

School of Medicine, Shahid Beheshti University of Medical Sciences Research in Medicine 1735-5311 48 4 2025 2 1 The Effects of Tramadol on Neurological Diseases (A Review Article) 75 81 FA Abbas Aliaghaei Department of Cell Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran Shayan Sadrinasab Department of Cell Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Background and Aim: Neurological diseases such as Alzheimer's and Parkinson's are among the most important diseases that, in addition to cognitive and motor effects, are often associated with chronic pain. Tramadol is used as an analgesic drug in the management of pain in these patients, but its effects on the central nervous system and cognitive and motor functions are still debated. Methods:The type of study is review article. Data collection is taken from reliable scientific databases such as Pubmed. This article examines the mechanisms of Tramadol's effect and its effects on Alzheimer's and Parkinson's patients. Considering the analgesic effects of tramadol through the inhibition of serotonin and norepinephrine reabsorption as well as the activity on opioid receptors, its side effects such as cognitive disorders, drowsiness and movement problems in these patients should be carefully considered. Conclusion:Studies show that tramadol can aggravate cognitive function in Alzheimer's patients and increase movement problems in Parkinson's patients. It seems that tramadol can be useful as a treatment option to reduce pain in patients with neurological diseases such as Alzheimer's and Parkinson's, but the issues related to drug side effects should also be considered.