Volume 48, Issue 3 (12-2024)
Research in Medicine 2024, 48(3): 21-29 |
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Ethics code: IR.IAU.QOM.REC.1398.019
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Raeisi J, Zolfaghari M R, Oloomi M, Siadat S D, Zargar M, Salehi Z. Species- Level Analysis of Blood Microbiota in Healthy Volunteers at the Pasteur Institute of Tehran Using 16S rRNA Sequencing. Research in Medicine 2024; 48 (3) :21-29
URL: http://pejouhesh.sbmu.ac.ir/article-1-3381-en.html
URL: http://pejouhesh.sbmu.ac.ir/article-1-3381-en.html
Javad Raeisi 
, Mohammad Reza Zolfaghari , Mana Oloomi , Seyed Davar Siadat , Mohsen Zargar , Zahra Salehi
, Mohammad Reza Zolfaghari , Mana Oloomi , Seyed Davar Siadat , Mohsen Zargar , Zahra Salehi
Department of Microbiology, Faculty of Basic Science, Qom Branch, Islamic Azad University, Qom, Iran , zolfagharymreza@gmail.com
Abstract: (288 Views)
Background and Aim: The significance of the microbiota as an ecosystem in our bodies has been well established. Recent studies have reported the presence of bacteria in the blood of healthy individuals. Our study aims to characterize the bacterial microbiota in human blood through 16S rRNA region sequencing. This study highlights the importance of method selection by focusing on the strains cultured in growth media.
Methods: This descriptive study collected 50 blood samples from healthy volunteers, following previous research protocols. In this study, a healthy individual is defined as someone who meets the following criteria: absence of chronic diseases or active infections. Blood samples were cultured in the Brain Heart Infusion (BHI) broth medium under controlled conditions. DNA was extracted from the resulting colonies. The 16S rRNA region was amplified, and amplicons were sequenced in both directions.
Results: Five out of fifty (10%) blood samples exhibited bacterial growth. All samples with bacterial growth were from females aged 25-43 years. BLAST analysis of the bacterial isolates revealed Staphylococcus epidermidis was identified in two samples with similarity percentages of 97.7% and 97.78%, respectively. Staphylococcus hominis was detected in one sample with a similarity of 99.05%. Additionally, a Bacillus species was found in one sample with a similarity of 98.62%, and Bacillus subtilis was identified in one sample with a similarity of 91.28%. Statistical analysis using Fisher's exact test did not reveal a significant relationship between age and the isolated bacterial strains
(P-value > 0.05).
Conclusion: It seems that this species identification method can be used for diagnosis purposes. It recommends further research
Methods: This descriptive study collected 50 blood samples from healthy volunteers, following previous research protocols. In this study, a healthy individual is defined as someone who meets the following criteria: absence of chronic diseases or active infections. Blood samples were cultured in the Brain Heart Infusion (BHI) broth medium under controlled conditions. DNA was extracted from the resulting colonies. The 16S rRNA region was amplified, and amplicons were sequenced in both directions.
Results: Five out of fifty (10%) blood samples exhibited bacterial growth. All samples with bacterial growth were from females aged 25-43 years. BLAST analysis of the bacterial isolates revealed Staphylococcus epidermidis was identified in two samples with similarity percentages of 97.7% and 97.78%, respectively. Staphylococcus hominis was detected in one sample with a similarity of 99.05%. Additionally, a Bacillus species was found in one sample with a similarity of 98.62%, and Bacillus subtilis was identified in one sample with a similarity of 91.28%. Statistical analysis using Fisher's exact test did not reveal a significant relationship between age and the isolated bacterial strains
(P-value > 0.05).
Conclusion: It seems that this species identification method can be used for diagnosis purposes. It recommends further research
Keywords: Blood Microbiota, Bacterial Diversity, Iran, Staphylococcus epidermidis, Blood Transfusion
Type of Study: Original |
Subject:
Microbiology
Received: 2024/05/25 | Accepted: 2024/08/21 | Published: 2025/01/20
Received: 2024/05/25 | Accepted: 2024/08/21 | Published: 2025/01/20
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