Research in Medicine

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Background:  todays, there are considerable efforts to finding plant substances as new drugs in cancer therapy. Scrophularia striata plants are a well-known in traditional medicine in Iran. This prompted us to investigate the anti-tumor effects of Scrophularia striata (an Iranian species) extract on human colorectal cancer (HT29) and normal (HEK293) cell lines.

Methods: In this experimental study,extract of Scrophularia striata was prepared by maceration method. Then, the cytotoxicity effect of different concentrations of Scrophularia striata extract was analysis by MTT assay on HT29 and HEK293 cells and IC50 for extracts was determined. Statistical analysis was done using SPSS software. The resulting data was analyzed using ANOVA test.
Results: Results of this study showed a significant cytotoxicity activity of Scrophularia striata extract against HT29 cells in a dose-dependent manner. So, Scrophularia striata extract at a concentration of 10 mg/ml to 0.1 mg/ml have toxic effects on the HT29 cells (P<0.05). However, this extract doesn’t have any cytotoxic effect on normal cells.
 
Conclusion:The Scrophularia striata extract has toxic effects on tumor HT29 cell line, so it can be used as an anti-cancer drug with specific concentration and low side-effects.
 

Keywords: Anticancer effect, colorectal cancer, Scrophularia striata, MTT assay

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