Volume 27, Number 1 (3-2003)                   Research in Medicine 2003, 27(1): 23-27 | Back to browse issues page

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Extraction of morphine from human urine using Liquid-Liquid-Extraction method and solid phase and their comparison with Slit scanning densitometry. Research in Medicine. 2003; 27 (1) :23-27
URL: http://pejouhesh.sbmu.ac.ir/article-1-177-en.html

Abstract:   (8392 Views)
Abstract Background: There are various methods for morphine extraction and detection, for this, Thin Layer Chromatography (TLC) is a useful tool. The most common detection technique of TLC plates is slit scanning densitometry. The aim of the present study was to compare the ability of a solid phase extraction (SPE) system with liquid-liquid extraction (LLE) for urinary detection of morphine by a TLC scanner. Materials and methods: Ten urine samples were collected from opioid abusers and each sample was divided in 4 two fractions were extracted by the SPE system and the other two were extracted by LLE technique (Choloroform/2-Propanol, shaking and centrifugation). One sample of each fraction was hydrolysed. Finally, all samples were quantitatively analyzed by TLC-scanner. Results: Results have revealed that the area under curve (AUC) on hydrolyzed samples was higher than nonhydrolyzed ones. Conclusion: Both techniques have showed better results for hydrolyzed samples. The recovery rate of SPE and LLE techniques were 94 and 80% respectively.
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Type of Study: General | Subject: General
Received: 2003/01/1

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