Volume 31, Issue 1 (spring 2007)                   Research in Medicine 2007, 31(1): 33-37 | Back to browse issues page

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Ghasemi A, Hedayati M, Biabani H, Khoshbaten A, Asgari A. Comparing deproteinization methods for serum nitric oxide assay by Greiss reaction. Research in Medicine 2007; 31 (1) :33-37
URL: http://pejouhesh.sbmu.ac.ir/article-1-352-en.html
, Ghasemi@erc.ac.ir
Abstract:   (15093 Views)
Background: Nitric oxide (NO) is involved in numerous physiologic and phathophysiologic processes. Recently, further investigators have focused on serum NO determination. In our previous study, we validated a simple, cheap and rapid method for serum NO determination based on the Greiss reaction. Deproteinization is a necessary step for this reaction, thus, the present study was designed to assess different deproteinization methods for serum NO determination. Materials and methods: Ten common protein precipitating chemicals including methanol, ethanol, zinc sulfate, methanol/diethyl ether, acetonitrile, TCA, PCA, sodium tangstate, ammonium sulfate and filter were used for deproteinization of 42 human sera, while results were compared to filter separation as a reference. Serum NO levels were determined in 60 sera of adult human. Results: Data showed that correlation coefficient of precipitating agents: methanol, ethanol, zinc sulfate, methanol-diethylether, acetonitrile, TCA, PCA, sodium tangstate, ammonium sulfate against filter separation method were 0.84, 0.92, 0.91, 0.79, 0.88, 0.85, 0.93, 0.53, and 0.78, respectively (p<0.001). Methanol, ethanol and methanol/diethylether caused overestimation, while TCA, PCA, sodium tangstate, and ammonium sulfate caused underestimation of serum NO results. Serum NO level had normal distribution with mean ±SE of 33±1.3 μmol/L. Conclusion: Although different chemical protein precipitants are used for serum NO determination, our study revealed that zinc sulfate is the best choice for this purpose.
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Type of Study: Original |
Received: 2007/10/20 | Published: 2007/04/15

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