Dr Matineh Farhadi, Dr Ensieh Lotfali, Dr Sareh Farhadi Farhadi, Mrs Maryam Jolehar ,
Volume 44, Issue 3 (9-2020)
Abstract
Background:
Candida species have been associated with the emergence of resistant strains against antifungal agents, recently. Antifungal agents are usually accompanied with side effects. Plant products have been used traditionally as alternative medicine to cure candidiasis. So it seems vital to find the new antimicrobial agents with fewer side effects. In this survey, we aimed to evaluate the antifungal effects of aqueous extracts of Mentha longifolia against oral pathogenic candida species.
Methods:
This study was conducted on
Candida albicans ( ATCC 10261), and ten
Candida albicans isolated from leukemia (AML and ALL) patients with thrush.
The antifungal activity and minimum inhibitory concentration (MIC) of Mentha longifolia extract were evaluated and then MIC results compared with
Chlorhexidine and nystatin.
Results: Mentha longifolia extract showed antifungal properties. MIC results of extract were in the range of 31.25 to 125 µg/ml. while MIC of Chlorhexidine were in the range of 125 to 250 µg/ml. Moreover, a significance difference was not observed between MIC of standard species and clinical isolates of
Candida albicans (P>0.05). According to the results, aqueous extract of Mentha longifolia was more successful in inhibition of growth of
Candida isolates, compared to
Chlorhexidine (P<0.001).
Conclusions: The inhibitory effects of Mentha longifolia extract have shown that it has potential to be considered as a promising candidate for the development of antifungal agent for treatment of thrush.
Mis Elham Kazemi, Mr Saham Ansari, Mis Sedigheh Sheikhzadeh, Mr Jalal Jafarzadeh, Mr Mojtaba Taghizadeh Armaki, Mis Samaneh Gharekhani, Mr Mohammad Chehrazi, Mr Abazar Pournajaf,
Volume 47, Issue 1 (4-2023)
Abstract
Background and Aim: Oral candidiasis is the most common opportunistic infection of the oral mucosa with Candida spp. Candida species form a biofilm in patients who need orthodontics with removable plaque, and this structure can lead to Candidemia. It is important to find effective plant compounds, which have fewerside effects and are natural, to prevent biofilm formation. The aim of the present study was to determine the effect of adding Thymus vulgaris and Cinnamomum verum essential oils to self- cure acrylic blocks on Candida albicans colonization.
Methods: In this experimental study, the antifungal activity of essential oils of Thymus vulgaris, Cinnamomum verum and nystatin against of Candida albicans ATCC10231 was evaluated in order to determine MIC according to CLSI-M27S4 guidelines. Powder and monomer were poured into tablet molds to make self-cure acrylic plaques. A total of 144 self- cure acrylic plaques were divided into 24 groups of 6 in petri dish. Three groups contained powder and monomer, three groups contained nystatin drug, and 18 groups contained different concentrations of essential oils of Thymus vulgaris and Cinnamomum verum. Then, the samples were incubated in artificial saliva enriched, with Candida albicans at 37°C and the effects of essential oils of Thymus vulgaris and Cinnamomum verum on the binding of Candida albicans were reported by the cfu/ml count method. Statistical analysis of data was done using Stata-15 software. ANOVA and Tukey's test were used to confirm the results.
Results: The evaluating the sensitivity of Candida albicans ATCC10231 to essential oils of Thymus vulgaris, Cinnamomum verum and Nystatin were obtained as MIC= 120µg/ml, MIC= 340µg/ml and MIC= 1µg/ml, respectively. The attachment of yeast cells to self- cure acrylic blocks for Cinnamomum verum essential oil was significant in MIC= 340 µg/ml (p= 0.02), MIC= 680 µg/ml (p= 0.01) and nystatin 1 µg/ml (p= 0.00) compared to the negative control group. However, this amount was not statistically significant for Thymus vulgaris essential oil (p= 0.14).
Conclusion: Adding essential oil of Cinnamomum verum to acrylic resin prosthesis can be effective in controlling the proliferation of Candida albicans on the surface of the prosthesis. However, its effects on the physical properties of acrylic resin dentures need further studies.
