Dr Abbas Ali-Shirood, Dr Mahmood Jamshidian, Dr Taghii Zahraei Saleh, Dr Gholam Reza Nikbakht Boroujeni, Dr Kumarss Amini,
Volume 40, Issue 2 (8-2016)
Abstract
Background: Salmonellosis is a zoonotic disease of human and animals. One of the most mportant serovars in human, livestock and poultry is Salmonella enteritidis which is a worldwide common foodborne pathogen. The aim of this study was differentiation and to determine the relationship among different isolates of Salmonella enteritidis by molecular fingerprinting method based on repeated sequences (rep-PCR).
Materials and Methods: In a cross-sectional study, 64 isolates of Salmonella Enteritidis from various sources (beef, poultry and humans) were studied by rep-PCR method. The molecular patterns were determined on the basis of the existence or absence and the size of bands. By using a computer program NTSYS, dendrogram were drawn.
Results: The number of 5-14 bands with the approximate size of 100 to 3,000 base pairs with a common bond (bp 400) were observed. The number of molecular profiles obtained by REP1R and REP2I primers for 64 isolates of Salmonella Enteritidis were 38 profiles.
Conclusion: The results showed that most strains of Salmonella Enteritidis isolates from human were classified separately from non-human (poultry and beef) isolates and the high genetic heterogeneity was observed.
Mrs Maryam Iravani, Dr Roya Mahinpour, Dr Zohreh Zahraei, Dr Zeinab Toluei,
Volume 43, Issue 3 (10-2019)
Abstract
Background: Today, due to increased use of chemical drugs and spread of microbial resistance to antibiotics as well as the side effects of drug consumption, the identification and introduction of plant species with medicinal and antimicrobial properties have widely been regarded important. Different species of salvia L. have been used in traditional and modern medicine for therapeutic purposes. In the current study, the antimicrobial activity of the essential oils and methanolic extracts of aerial parts of four species of Salvia contains S. syriaca L. and S. ceratophylla L and two populations of S. reuterana Boiss., and two populations of S. limbata C.A. Mey. from Kashan region, were investigated.
Materials and Methods: An experimental research was conducted in in vitro conditions. Methanolic extract of samples was prepared using soxhlet apparatus. The essential oils were extracted using hydrodistillation using Clevenger method. Antimicrobial activity of this species was investigated using the Agar well diffusion technique and MIC and MBC tests.
Findings: The essential oils and methanolic extracts of these sample plants showed antimicrobial activity against Bacillus subtilis, Staphylococcus aureus, and Staphylococcus epidermidis in 30 mg/ml. In addition, extract of S. reuterana and essential oil of S. limbata from Dorreh inhibit the growth of Candida albicans.
Conclusion: It seems that considering the antimicrobial properties of some of the extracts and essential oils observed in the current study, they can be used as substitutions for the current antibiotics after more extensive graduate studies are performed.
