F Fallah, G Eslami, J Khoshnevis, H Goudarzi, M Habibi, E , Azargashb, ,
Volume 30, Issue 4 (12-2006)
Abstract
Background: Diabetic foot infections are a potentially severe complication of diabetes. Diabetic foot infections can sometimes lead to long-term debilitation and, in the most severe cases, amputation. They are the most common infections in patients with diabetes, whose weakened immune systems put them at an increased risk of acquiring antibiotic resistant infections.
Materials and methods: For this descriptive study, 120 diabetic patients (30 women and 90 men age ranged between 45-65 years and disease duration of 0.5 to 37 years) were investigated. Immediately after the hospitalization, specimens from infected foot lesions were taken using Thio and BHI as transport medium. Aerobic cultures were carried out in all cases according to conventional methods while anaerobic cultures were performed when appropriate. Finally, susceptibility tests were performed on isolated microorganism.
Result: Totally, 75% of cases were polymicrobial infections. We isolated gram positive cocci 95%, gram positive bacilli 35%, gram negative 55% and mycobacterium 10%. Meanwhile, we found that 12.5% of our bacteria were anaerobic and 87.5% were facultative aerobic bacteria. In antimicrobial susceptibility testing, Rifampin was the most effective antibiotic against S.aureus and peptostreptococcus. Surprisingly, E.coli was resistant to all tested antibiotics.
Conclusion: Diabetic foot infections have a polymicrobial nature. Antibiotic treatment of infections should be prescribed on the results of microbiological investigation.
, , Fatemeh Fallah1, Seyed Ali Mirdehghan2, Nina Faramarzi1, , , , ,
Volume 33, Issue 2 (11-2009)
Abstract
Abstract
Background: Conjunctivitis, one of the most prevalent eye complications, is usually self limited but may result in optical disorders .Classification is based on the cause including bacterial, viral, fungal, allergic or chemical. Considering antimicrobial resistance, determining the exact cause may lead to improved medical therapy.
Methods: In this descriptive-analytic survey, 92 patients with conjunctivitis, who attended Shaheed Labbafi Nezhad medical center were enrolled. Samples were accurately collected by the physician working in the center and were transported in a sterile condition to the Laboratory of Pediatric Infectious Research Center of Mofid hospital. Determination of mycobacteria, aerobic and anaerobic bacteria was accomplished using standard methods. Data was analyzed using SPSS 13.
Results: Samples were obtained from 49 men (61.4%) and 43 women (38.6%), (median age 36.2). 85% of patients with bacterial conjunctivitis had mucoplurant discharge as a main symptom. 45(58.5%) cultures were positive. Aerobic organisms were isolated from 40 patients (57.1%) and anaerobic ones from 5 (7.1%), of which 4 were mixed with aerobes. No mycobacterium was found. The most common aerobic organism cultured was staphylococcus epidermidis (30%) and the other aerobic ones were: Staphylococcus aureus (12.9%), E coli (7.1%), Bacillus cereus (5.7%), Moraxella catarrhalis (4.3%), Diphteroid (4.3%), Acientobacter baumanii (2.9%), Citrobacter fraundii (2.9%), Staphylococcus oricularis (1.4%), Streptococcus viridans (1.4%), Bacillus subtilis (1.4%), Pseudomonas aeruginosa (1.4%), and Proteus mirabilis (1.4%). Anaerobic organisms cultured were Peptostreptococcus (4.3%) and Bacteroides fragilis (2.9%).
Conclusion: Since various organisms are responsible for bacterial conjunctivitis, therapeutic strategies should be based on the results of microbiological investigations.
KEYWORDS: Conjunctivitis, Mycobacterium, Aerobic bacteria, Anaerobic bacteria.
