Mrs Fatemeh Sameni, Mr Ali Esmaeili, Dr. Hossein Dabiri, Dr. Robabeh Azargun, Dr. Hossein Goudarzi, Dr. Alireza Mohammadzadeh,
Volume 44, Issue 1 (3-2020)
Abstract
Abstract
Pseudomonas aeruginosa is an opportunistic pathogen and management and treatment of infections caused by drug resistant strains of P. aeruginosa has become a major challenge(1). The aim of the present study was to investigate the prevalence of resistant isolates of P. aeruginosa class I integron in clinical and environmental samples.
Materials and Methods: In current descriptive study on 101 samples, patients with cystic fibrosis admitted to the pediatric ward and patients with burn infections were included. Samples from ulcers and throat were collected and analyzed by microbiological and biochemical methods. Antibiotic susceptibility testing was performed by disk diffusion method according to CLSI guidelines. DNA was extracted and gene for class I Integrons was amplified.
Results: Of 101 strains isolated from patients (37 from cystic fibrosis and 64 from burn patients) which drug susceptibility was performed, 97.02% was susceptible to colistin and 30.6% was susceptible to amikacin and Pipracilin. Most resistant drug shown for ceftizoxime 97.2% and Ceftriaxone 67.3%. From the isolated strains, 18 isolate show complete resistant, 7 intermediate resistant and 76 were pan-susceptible. According to our analysis, 50% of isolate have MDR resistance. Based on PCR analysis, of 101 isolates, 60 (%59.4) have integron class 1 gene. This gene was significantly associated with strains resistant to amikacin, tobromycin, ciprofloxacin and pipracillin.
Conclusions: there was an increasing concern about the resistance of P. aeruginosa to the most available antibiotics such as colistin, amikacin and imipenem.
