Showing 18 results for Live
Isa Neshandar Asli ,
Volume 14, Issue 1 (3-1990)
Abstract
Recently the incidence of visceral Jarva migrans has increased in the northern states of Iran. Eight infected patients either from northern states of with a history of the trip to these states, were evaluated in Taleghani Hospital, Shaheed Beheshti University with chief complaints of mild fever, malaise, anorexia, weight loss, hypochondria! pain. High level eosinophilia (more than 20%) in all of them and increased hepatic enzymes in one case were considered. Isotopic liver and spleen scan in these patients indicated abnormal hepatic image as a single or multifocal areas with decreased activities, whereas hepatic ultrasonography demonstrated homogeneous ech1oic distribution, in all of them. This study revealed the values of isotopic liver and spleen scan, because of: 1- Demonstrating hepatic gran ulomatous involvement in spite of normal sonography and hepatic enzymes; 2- Hepatic granulomas may persist for months or years (1,2,3), if these patients be surveyed for other diseases such as metastasis, their hepatic scintigraphic defects may disinterpret as metastasis, therefore, having an isotopic liver and spleen scan as a base seems to be necessary in the tim of this infectious involvement.
, Farzad M, Ghazi Mirsaeed Sb ,
Volume 29, Issue 4 (12-2005)
Abstract
Background : In order to assess whether maternal blue is attributed to the rout of childbirth including vaginal delivery or cesarean section, this study was conducted at Hedayat Hospital in 2000.
Materials and methods : Among women who attended to the clinic of prenatal care in Hedayat Hospital those who accepted to participate were requested to fill a Beck depression inventory to rule out any underlying depressive disorder. Finally, for 200 parturients a structured questionnaire was filled out, once before and again one week after delivery. Initial data including age, education level, planned or unplanned pregnancy, familial satisfaction were inquired.
Results : Results showed that 55% of the subjects had symptoms of maternal blue during post partum period. Maternal blue is more prevalent among women who had cesarean section compared to vaginal delivery candidates (p<0.08, RR=1.6) however, the symptoms of maternal blue was less serious compared to mothers with vaginal delivery.
Conclusion : Maternal blue was revealed to be prevalent among parturients, thus further studies are warranted to address the problem and preventive interventions.
, , , , Hossein-Panah F, Sadeghi L, Rambod M, Foroutan M, Naseri M ,
Volume 30, Issue 1 (3-2006)
Abstract
Background: Non-alcoholic fatty liver disease (NAFLD) is a relatively prevalent disorder with many suspected risk factors. The aim of this study was to assess the association between the suspected risk factors with the presence of NAFLD according to the ultrasonographic criteria in type II diabetic patients.
Materials and methods: The study was performed on 76 type 2 diabetic patients attending consecutively to endocrine clinic. Negative history of alcohol use and hepatitis B and C were our basic inclusion criteria. All candidates underwent thorough clinical and biochemical assays consist of height, weight, waist circumference, fasting blood sugar, HbA1c, triglyceride, total cholesterol, HDL and LDL, liver trans-aminases, alkaline phosphatase, thyroid function tests, serum iron and total iron binding capacity and liver sonography to detect the presence (stage I to III) or absence of steatosis. The score of metabolic syndrome was determined according to the latest ATP III criteria. Pearson and Spearman correlation coefficients and logistic regression were used to assess the relationship between suspected risk factors and the presence of NAFLD.
Results: Forty-nine patients (64.4%) were female. The mean age (± standard deviation) was 59.7±8.8 years. The mean BMI in patients with and without NAFLD were 29.4±4.5 and 24.8±3.8kg/m2, respectively (p<0.001). Prevalence of NAFLD was significantly higher among patients with metabolic syndrome (89% vs. 43%, p<0.05). Age (r=-0.24, p<0.05), waist circumference (r=0.27, p<0.05) and triglyceride (r=0.28, p<0.05) had significant correlation with steatosis. However, in logistic regression analysis, only BMI (for each 5 unit increment) was independently associated with the presence of NAFLD (OR=4.25, 95%CI: 1.7-10.9, p<0.05).
Conclusion: In type 2 diabetic patients, only BMI had a significant role in predicting NAFLD. It seems that other metabolic factors will not give any additional information for predicting NAFLD.
F Yassaee, B Mohseni,
Volume 31, Issue 2 (9-2007)
Abstract
Background: The rate of cesarean section in our society is increasing every day. This research was done to study the effects of mother’s personal preference about the mode of delivery on the outcome of delivery in pregnant women who recieved perinatal care in Taleghani and Mahdieh Hospitals.
Material and methods: This cross-sectional study was done on 160 women pregnant with there first babies. Mothers’ characteristics: First pregnancy, no systemic disease, no indication for cesarean section before labor pain, no history of infertility, non-smokers. All subjects were interviewed about their preferences regarding the method of delivery.
Results: Ninety-four women, (58.8%), preferred to have a normal vaginal delivery, and 66, (41.2%), chose cesarean section. From the first group, 65 (61.3%) cases and from the second group, 41 (38.7%) cases came back to the same hospital for delivery. The rate of cesarean section in the first group was 12.3% and in the second group was 34.1% (P<0.01).
Conclusion: There is a positive association between mother's wish about the method of delivery and it’s outcome.
Fariborz Hovanloo, Mehdi Hedayati, Maryam Ebrahimi, Hossein Abednazari,
Volume 35, Issue 1 (5-2011)
Abstract
Abstract
Background: Anti oxidative enzymes activity is an important issue in oxidative stress. Most previous researches have dealt with the effect of brief and anaerobic exercise on these enzymes the purpose of this study was to determine the alterations of antioxidant enzymes viz. superoxide desmutase, catalase and glutathione (SOD, CAT, and GPX) activity after endurance training in rat liver tissue.
Methods: In this experimental study, 62 male Wistar rats were selected randomly and divided into 3 control and 3 experimental groups. Experimental rats trained for 6, 9 and 12 weeks, 5 days/week running on treadmill with initial speed of 10 m/min for 10 minute to the ultimate speed of 25 m/min for 60 minute. For control groups the protocol was walking on treadmill with 10 m/min speed for 3 days/ week. After each course (6, 9, 12 wk.), rats were anesthetized and samples taken from the livers of all animals in the study. Determination of antioxidant enzymes activity was done with enzymatic color assay method.
Results: The results showed that 6, 9 and 12 weeks of endurance training have no significant effect on GPX activity. Also, 6 and 9 weeks of endurance training did not alter the activity of SOD and CAT in liver tissue, but, 12 weeks of endurance training decreased SOD and CAT activity significantly, (CAT activity in experimental group was 23.11 ± 7.27 U/mL and in control group it was 31.43 ± 7.21 U/mL SOD activity in experimental group was 47.70 ± 0.56 U/mL and in control group it was 48.20 ± 0.48 U/mL, (P<0.05)).
Conclusion: Results of this study reveal that participating in 9 weeks of moderate exercise does not have detectable effects on the anti oxidative system, but when the exercise continues until 12 weeks, it decreases the SOD and CAT enzyme activity.
Keywords: Endurance training, Superoxide dismutase, Catalase, Glutathione peroxidase, Liver, Rat.
Karam-Ali Kasiri, Mohsen Dehghani, Anahita Sadeghian,
Volume 37, Issue 3 (11-2013)
Abstract
Background: Lymph proliferative is the popular disease after organ transplant. The prophylactic use of ganciclovir is one of the solutions in preventing this disease. In this study, the effect of prophylaxis ganciclovir in the prevention of PTLD in children was examined.
Materials and Methods: In this cohort study, case group of children who had undergone a liver transplant in January 2011 to March 2012 have been received prophylaxis ganciclovir. The control group of children, who underwent liver transplantation from March 2009 to December 2010, not received prophylaxis ganciclovir. The incidence of PTLD and mortality in the two groups were compared. Patient follow-up in relation to lymph proliferative disease and its side effect was carried out. Background information was obtained from the patient’s file. The data was analyzed by SPSS software.
Results: In total, 203 children with liver transplant were entered into the study. Control group of 108 children, 45 girls and 63 boys. The mean age of control group was between 8.7± 5.6 and in the case group was between 9±5.6. Case group of 95 patients, 37 were girls and 58 were boys. In the control group, 12 (11.1%) and in case group 5 (5.3%), PTLD were diagnosed (p<0.02). The mortality rate in the control group was 5(4.6%) and in the case group was 0. The difference was significant (p<0.05).
Conclusion: Prophylaxis ganciclovir is effective in decreasing the rate of PTLD and the mortality resulting from that in children after transplant.
Dr Saeed Nouri, Dr Mohammad Reza Sharif, Dr Yunes Panahi, Dr Mostafa Ghanei, Dr Bardia Jamali,
Volume 38, Issue 4 (1-2015)
Abstract
Background: The control of parenchymal hemorrhage especially in liver parenchyma, despite surgical science progresses is still one of the challenges surgeons face saving the patients’ lives and there is a research challenge between the researchers in this field to introduce a more effective method. This study aimed to determine the hemostatic effect of calcium sulfate on controlling the bleeding from liver parenchymal tissue.
Materials and methods: In this animal model study 60 male Wistar rats were used. A length of two cm and a depth of half a cm incision were made on each mouse’s liver and the hemostasis time was measured using calcium sulfate different concentrations (5%, 10%, 15%, 25%, and 50%) and the control method (i.e. control of bleeding by suturing). Finally, the obtained data were entered into SPSS software and analyzed using Kruskal-Wallis test and Mann-Whitney, Kolmogorov-Smirnov.
Results: The hemostasis time of calcium sulfate concentration groups was significantly less than that of the control group (p<0.01).
Conclusion: Calcium sulfate is an effective hemostatic agent in controlling liver parenchymal tissue hemorrhage in an animal model.
Eng Bahman Mohammadkhani, Dr Hadi Tabesh, Dr Behzad Houshmand, Dr Behrouz Mohammadkhani,
Volume 40, Issue 3 (11-2016)
Abstract
Zeolite is an active biomaterial which has also biocompatible, antibacterial non-toxic and high porosity properties. In recent years many investigations have been performed on its application in different fields of sciences. In this study, a systemic review on zeolite application in advanced medical sciences and introduction of potential zeolite types for such uses. Investigating the literatures, zeolite’s applications in medicine were classified in different fields e.g. pharmaceutics, orthopedics, tissue engineering, and dentistry. It was also clarifies that the proper selection of a zeolite type is crucial for any specific research activity since they demonstrate diverse physico-chemical properties. Zeolites are edible, antibacterial, biocompatible and non-toxic material with high absorption property, whose application in different medical fields are expanding. Choosing zeolite, either synthetic or natural, can be a proper selection for e.g. drug delivery systems, wound healing, scaffolds in tissue engineering, implant coatings, hemodialysis, gas adsorption and elimination of toxic ions. In order to bring all these application under an umbrella, two classifications based on application types biological and physico-chemical properties of zeolites have been presented which provides an overview on medical applications of zeolites and also helps scientists in better selection of zeolite type for their research activities.
Mrs Nourossadat Kariman, Sanaz Fayazi, Miss Zohre Sheykhan,
Volume 41, Issue 1 (4-2017)
Abstract
Background: Gynaecologists and midwives as a practical model of childbirth have an essential role in
education. The aim of this study was to determine the attitude toward labor and preferred delivery method
among midwifery and medical students and other related factors at medical universities in Tehran in 1393.
Methods: We carried out a descriptive study on 300 students of Medical Sciences Universities in Tehran
in 1393. For data collection tool we used two researcher made questionnaires contain “demographic
characteristics and related factors to the preferred mode of delivery“ and “attitude toward delivery mode”.
To investigate the reliability, two methods of test-retest and internal consistency (Cronbach’s Alpha) were
applied and the content validity was determined quantitatively and qualitatively. The data were analyzed by
SPSS-16 software. Statistical tests used included: chi-square, T-test, ANOVA or non-parametric equivalents
of them. The significance level accepted in this study was p-value>0.05.
Results: Most of the students (49%) preferred caesarean section for themselves. In comparison of 4 groups
first year midwifery (16.7%) and medical students (48.6%) had preferred caesarean less than fourth year
midwifery students (51.3%) and medical interns (%75.7). The majority of students had a positive attitude via
caesarean section. Therefore, the four study groups respectively 45.5%, 92.3%, 30.8% and 100% of first and
fourth year midwifery students and first year medical students and medical interns had a positive attitude
toward caesarean delivery. Among the relevant factors under investigation age, ethnicity, weight, body mass
index, birthplace, residence place and family income were significantly associated with the preferred mode
of delivery.
Conclusion: The caesarean section is preferred and a positive attitude towards it among midwifery and
medical students is very high and the education system authorities should create plans to improve students’
attitudes.
Kolsoum Afsordeh, Rouhollah Ranjbar, Aliakbar Alizadeh,
Volume 43, Issue 1 (9-2019)
Abstract
Backgrond:Fatty liver, which impairs the balance of blood lipids, is one of the most common liver diseases
in the present century. The purpose of the present study was to investigate the effect of aerobic exercise and
vitamin D supplementation on fatty liver and lipid profiles.
Materials and Methods: In this semi-experimental study, conducted in the winter of 1395 in the city of Dehloran,
40 women (weight: 81.5±8.8 and BMI: ≥30) who had Fatty Liver, grades I to III, were randomly divided into
aerobic exercise and vitamin D supplement (combined), aerobic training, Vitamin D Supplementation, and
control groups (n=10). The exercise groups performed 45 minutes of exercise on treadmill with 60% maximum
heart rate for 8 weeks and 3 sessions per week. Supplementary groups received 50,000 units of vitamin D every
week. Blood samples were used to measure lipid profiles and liver enzymes and ultrasound to measure liver
parenchyma in pretest and posttest (48 hours after the latest session of aerobic training). Descriptive statistics
were used to determine the mean and standard deviation of each variable and Shapiro-Wilkes test to determine
the natural distribution of data. Also, covariance analysis was used to examine the changes between groups.
In order to compare the two groups, Bonferron’s post hoc test was used.
Results: The hepatic enzymes in the combined group and the aerobic training group were significantly
different compared with the control and vitamin D supplementation groups (p<0.05) (ALP=0/001, ALT=0/014,
AST=0/000. However, it was not found to be statistically different between combined and aerobic training in
the hepatic enzymes. The liver parenchyma was significantly decreased in the combined group compared
with other groups (p<0.05) (P=0/000). In addition, Triglyceride and LDL levels decreased significantly in
the combined group as compared with other groups (p<0.05) (LDL=0/011, TG=0/015). However, there was
no statistically significant difference in the HDL and cholesterol between the groups (p>0.05) (HDL= 0/148,
COLE=0/591).
Conclusion: Vitamin D supplementation with aerobic exercise can have more effects on the levels of liver
enzymes, liver parenchyma, as well as triglyceride and LDL in women with fatty liver.
Keywords: Aerobic exercise; Vitamin D; Fatty liver; Liver enzymes; Lipid pr ofiles
Elahe Abooali, Mohammad Salehi,
Volume 44, Issue 2 (6-2020)
Abstract
Background: Helicobacter pylori infection is known as an important risk factor in the development of ulcer,
gastritis, and malignancy. However, recently, the systemic effect of H. pylori infection on other organs, such as
infection of liver and development of non-alcoholic fatty liver disease, has been found. The prevalence of HPI
in NAFLD patients has been noted, as the prevalence of NAFLD is growing worldwide. The aim of the present
research was to determine the prevalence of H. pylori in NAFLD patients. Then, in case of positive relationship,
through the easy and inexpensive control and treatment of H. pylori, it is possible to prevent development of
NAFLD whose prevalence is increasing.
Materials and methods: A descriptive cross sectional study was carried out. NAFLD patients referring to
Digestion clinic of Loghman Hakin hospital were chosen using available sampling. After confirmation of NAFLD
via ultrasound, serology test of H. pylori antibody (IgG) was requested. Then, the collected data were coded
and introduced into computer. The quantitative data are described using mean and standard deviation, while
the qualitative data are described by frequency and percentage. Chi2 and independent t-test were used for data
analysis. The prevalence was determined by confidence interval %95, and the statistical tests were performed
at significance level of %5.
Results: In the present study, 181 NAFLD patients were tested. The mean age of the patients was 11.95±44.15
years (male= %38.7( 70); female= %61.3( 111)). One patient (%0.55) from the NAFLD group was Grade IV, 15
%8.29() were grade III, %28.18( 51) Grade II, and most of the patients (%63 ,114) were Grade I. H. pylori was
positive among 114 patients (%63). The prevalence rates of positive IgG test among women and men were %64
and %61.4, respectively. No significant difference was observed in IgG test prevalence between men and women
(p=0.731). No significance difference existed between the age of patients in terms of IgG test results, either
(p=0.441). Although the prevalence of H. pylori was less in non-smokers, no significant difference was observed
in the prevalence of H. pylori in terms of smoking status. Finally, no significant difference was observed between
the two groups in terms of BMI (p=0.437).
Conclusion: Our study indicated that the prevalence of H. pylori is relatively high in NAFLD patients. This high
prevalence can be a cause for the possible relationship between H. pylori and NAFLD.
Sara Derakhshan, Ali Bidmeshki Pour, Reza Kowsari-Esfahan, Masoud Vosough, Leila Montazeri, Mohammad Hossein Ghanian, Mohamad Amin Hajari, Mohamad Ajoudanian, Abbas Piryaei,
Volume 44, Issue 4 (12-2020)
Abstract
Background and Aim: There is an urgent need for reliable in vitro liver models to assess the mechanisms of human diseases, and impact of newly developed drugs or toxins. Previously showed that co-culture of hepatocytes with non-parenchymal cells and presence of extracellular matrix (ECM) improve the in vitro functionality of hepatocytes. Aim of this study was to develop a reproducible method for scalable generation of liver microtissues.
Materials and Methods: In this experimental study, liver microtissues with 500um diameter generated through co-culturing of Huh-7, human mesenchymal stem cells and human umbilical vein endothelial cells in a composite hydrogel of liver-ECM and alginate. Cell viability, capability for glycogen storage and cytochrome P450 enzyme activity were evaluated on day 28. Hepatic-specific genes (ALB and SULT1A1) on days 1 and 14, and albumin and urea secretion on days 1, 7, 14, 21 and 28 were assessed. Furthermore, the microtissue sensitivity to acute toxicity of Metformin, Diclofenac and ethanol were investigated. Two-dimensional cultured Huh-7 was considered as control group.
Results: Most cells in the microtissues were alive up to day 28, accumulated glycogen and have cytochrome inducibility 7-fold more than the basic activity. In comparison to control, ALB and SULT1A1 genes upregulated (p<0.05) on day 14, also albumin and urea secretion increased (p<0.05), in all sampling time, in microtissues. Furthermore, microtissues have more sensitivity (p<0.05) to some concentrations of the drugs and ethanol, compared to control.
Conclusion: It seems that the liver microtissues may considered as an appropriate and functional in vitro model for liver.
Dr Hamdollah Iraji, Vazgen Minasian, Roya Kelishadi,
Volume 45, Issue 1 (3-2021)
Abstract
Background: Although lifestyle changes encompassing weight loss and exercise remain the cornerstone of non-alcoholic fatty liver disease (NAFLD) management, the effect of different types of exercise on NAFLD is unknown. So, the purpose of the present study was to evaluate the impact of eight weeks of High-Intensity Interval Training (HIIT) on liver lipids, liver enzymes, and insulin resistance of adolescents with fatty liver.
Materials and Methods: In the current experimental research, 23 children and adolescents with non-alcoholic fatty liver were randomly assigned into HIIT groups. The intervention was eight weeks of HIIT training consisting of 30 seconds of fast running at around 100–110 percent of max speed with a 30-second recovery interval, 3 sessions per week each session lasting for 32-36 minutes, whereas the control group had no regular physical activity. Variables were measured before and after the intervention in both groups. The data was analyzed running ANCOVA and Bonferroni post-hoc tests.
Results: Significant differences were observed in VO2peak (HIIT9.38% vs. Control 0.62%), BF percentage (HIIT;-1.25% vs. Control; 0.31), WHR (HIIT;-1.24% vs. Control; 0.41), ALT (HIIT;-12.42% vs. Control 3.84%), AST (HIIT;-13.95% vs. Control; 4.80%), insulin (HIIT; -12.37% vs. Control -3.56%) and insulin resistance (HIIT; -15.24% vs. Control -2.83%) between HIIT and control groups (P<0.05), whereas the differences in blood pressure and fasting blood sugar between the two groups were not found to be significant (P>0.05).
Conclusion: It seems that the HIIT exercise can be helpful as a non-medical intervention to improve liver enzymes and in the treatment of obese/overweight adolescents with non-alcoholic fatty liver.
Ms Masoume Sedighi, Dr Jabbar Bashiri, Dr Alireza Nourazar, Dr Hamdollah Hadi,
Volume 45, Issue 3 (9-2021)
Abstract
Background and Aim: Nowadays, considering the occurrence of various diseases as a result of inflammation, finding a solution for inflammations caused by various factors is very important. Therefore, the aim of the present study was to investigate the effect of eight weeks of endurance training and complementation on the levels of CD22 and TRL4 in obese wistar rats.
Materials and Methods: In an experimental study, 40 Wistar rats of two-month age range, were purchased from Pasteur Institute of Karaj and divided into four groups (each group including 10 animals) of high-fat diet without intervention, endurance training group, only olive supplement, and the olive supplement group with endurance training. Animals in the training groups underwent a two-month endurance training program (80% maximal oxygen consumption). Also, in the olive oil supplement group, one gram of olive oil per kg body weight was fed daily through oral gavage. ELISA method was used to evaluate serum TLR4 and CD22 levels, Shapiro-Wilk test was used to evaluate the normal distribution of samples, and one-way and two-way ANOVA were run for the purpose of between groups comparisons (p<0.05).
Results: The results showed that two months of endurance training alone do not have a significant effect on inflammation )TLR4 (P=0.09), CD22(P=0.16)) in obese male Wistar rats. However, consumption of olive oil alone significantly reduced TLR4 (P = 0.009) and CD22 (P = 0.008). This is while two months of endurance training with oil consumption had no significant effect on TLR4 (P = 0.09) and CD22 (P = 0.10).
Conclusion: It seems that consumption of olive oil can reduce obesity-induced inflammation (TLR4) to some extent. However, further research is needed to fully reveal this.
Ramesh Farokh Nezhad, Dr. Mitra Nourbakhsh, Dr. Maryam Razzaghy Azar, Dr. Roya Sharifi, Dr. Parichehreh Yaghmaei,
Volume 45, Issue 4 (12-2021)
Abstract
Background and aim: Prevalence of obesity has remarkably been elevated during the recent years and therefore its consequences, including liver and cardiovascular disorders, have increased. The previous studies have shown that trans-palmitoleic acid (tPA) has beneficial effects in metabolic disorders. Thus, the aim of the present study was to study the effects of tPA on lipid accumulation in liver cells and the gene expression of fatty acid synthase (FAS) enzyme and the activity of peroxisome proliferator-activated receptor alpha (PPARα).
Methods: In the present experimental in vitro study, HepG2 liver cells were cultured and treated with different concentrations of tPA and palimitic acid. Intracellular triglyceride (TG) levels were measured with an enzymatic method, and the expression of FAS gene was assessed using real-time PCR. The activity of PPARα was also evaluated via luciferase reporter assay, using the vector comprising the PPARα response element.
Results: Both fatty acids caused TG deposition in liver cells; however, the TG levels were significantly lower in cells treated with tPA compared to the cells that were treated with palmitic acid (P<0.001). The gene expression of FAS was enhanced by both fatty acids, but in cells treated with tPA, it was significantly lower than palmitic acid (P<0.001). Also, tPA was obserced to activate PPARα, especially at lower concentrations (P<0.001), while palmitic acid did not have any effect on this nuclear receptor.
Conclusion: The results showed that less TG is accumulated in liver cells treated with tPA as compared with palmitic acid and thus this fatty acid has better influence on liver cells compared with its saturated counterpart.
Amir Parviz Rezaei Saber, Hossein Taghilou, Saeed Aftabi, Reza Karimi Johani,
Volume 46, Issue 2 (6-2022)
Abstract
Background and Aim: Non-alcoholic fatty liver disease is a condition in which triglycerides accumulate in liver cells of individuals who do not have a history of alcohol use or use alcohol at a low rate. The aim of the current study was to investigate the effect of alcoholic extracts of St. John's wort and borage plants individually and in combination on non-alcoholic fatty liver in Wistar rats. These two plants were selected due to their innovation and high antioxidant characteristics.
Methods: In the present experimental laboratory intervention study, 70 male Wistar rats were randomly divided into 7 groups of 10, and 10 mice were randomly assigned as the negative control group and the rest were treated with a high-fat diet. Next, 21 days after the onset of a high fat diet and fatty liver, they began to be treated with an alcoholic extract of Silybum marianum and Eryngium and kept for 2 months individually and combined in two doses of at least 200 and a maximum of 400 mg in kg. At the end of the treatment period in 1398 and to evaluate the level of concentrations of unsaturated fatty acids (NEFA), non-esterified fatty acids (BHB: hydroxybutyrate-Beta), alanine aminotransferase (ALT), aspartate amino trans (AST) Aspartate aminotransferase, and gamma glutamyl transferase (GGT) blood samples were taken and the data were analyzed. The findings were analyzed using SPSS, version 24. The results were compared using one-way analysis of variance statistical test at 95% probability level and the significance level of 0.05.
Results: The results showed that NEFA, BHBA, ALT, AST, and GGT levels in the treated groups of alcoholic extract of Silybum marianum and Eryngium combination and single effect on fatty liver were more than those in the control group and positive control group (P <0.05). The amounts of AST in the control and positive, the negative, and combined control groups were 145.4 ± 9.1, 114 ± 11.4, and 136.1 ± 2.9, respectively.
Conclusion: It seems that thistle and buckthorn can be used to treat non-alcoholic fatty liver disease, which of course requires clinical trials in livestock, which improves the amount of liver enzymes due to the effect of these plants on liver tissue and improving the amount of related enzymes. Therefore, these plants can be used in future research on livestock and their effects on improving liver enzymes and protecting liver tissue.
Bahloul Ghorbanian, Safa Eghtesadi,
Volume 46, Issue 4 (12-2022)
Abstract
Background and Aim: Non- alcoholic fatty liver disease is characterized by fat deposition in liver cells and, if not controlled, may cause fibrosis, cirrhosis, and cell destruction of the liver. Sports activity is one of the contributing factors in the recovery of non-alcoholic fatty liver patients. The aim of this study was to investigate the effect of eight weeks of high- intensity interval training (HIIT) on serum levels of adiponectin, irisin, and lipid profile in women with non- alcoholic fatty liver disease.
Methods: In this experimental clinical trial, 25 women with non-alcoholic fatty liver disease with an age range of 30-50 years were selected as a statistical sample based on the inclusion criteria and randomly divided into two exercise groups (13 people) and control groups (12 people). Intense interval training was performed for 8 weeks (four sessions per week and each session lasting 48 to 60 minutes). Blood samples were taken before and after the test to measure the levels of adiponectin, irisin, and lipid profile. Statistical analysis of the data was done using independent t- test and correlated t- test using spss21 software at a significance level of p<0.05.
Results: The results showed that after training intervention, the serum levels of adiponectin (p= 0.049), irisin (p= 0.004), and HDL (p= 0.005) significantly increased, and LDL (p= 0.001), TG (p= 0.001), TC (p= 0.001) had a significant decrease in the exercise group compared to the control group (p≥0.05).
Conclusion: Considering the positive effects of HIIT on serum levels of irisin, adiponectin, and lipid profile in patients with non- alcoholic fatty liver, it seems that these exercises can be recommended as a non- pharmacological treatment to improve these patients.
Zahra Tahmasvand, Shima Lorestani, Shokoofe Noori,
Volume 47, Issue 1 (4-2023)
Abstract
Background and Aim: Non- alcoholic fatty liver is a disease that will lead to liver cirrhosis if not treated. Curcumin is the active substance of the rhizome of the turmeric plant, which has antioxidant, anti- inflammatory, antimicrobial, etc. properties. In the present study, the effects of curcumin analog on the expression of SIRT1 and FAS genes and the accumulation of triglycerides in fatty HepG2 cells (fatty liver model) have been investigated.
Methods: In this experimental study, after cultivating HepG2 cells in the right medium and conditions, the MTT test is used to choose the right dose for curcumin and it’s analog. After oiling the cells and treating them with the desired compounds, RNA was extracted and cDNA was made. Then, in order to investigate the effect of curcumin and its analog on the expression of SIRT1 and FAS genes, Real- Time PCR was used. SIRT1 activity was measured using a fluorometric kit and intracellular triglyceride levels were also measured using a triglyceride detection kit. And finally, the results were analyzed with t- test and one-way Anova statistical methods with p-value < 0.05.
Results: As a result of the treatment of HepG2 fatty cells (fatty liver model) with Cur, the relative expression of SIRT1 gene (1.52 ± 0.8) and its enzyme activity (2.1 ± 0.7) increased significantly compared to the control group (for gene expression: 0.9 ± 1.79 and for enzyme activity: 0.8 ± 1.66). On the other hand, the relative expression of the FAS gene (0.6 ± 1.69) compared to the control group (0.8 ± 2.03), as well as the accumulated triglycerides in these cells, and has decreased significantly. Also, in the group treated with FCur compared to the control group, the level of SIRT1 gene expression (2.76 ± 1.16) and enzyme activity (3.2 ± 1.21) increased significantly, and the level of FAS gene expression (0.2 ± 1.1) decreased significantly compared to the control group. The increase in SIRT1 enzyme activity in the FCur group has increased more compared to Cur, and the decrease in FAS gene expression in the treatment of cells with FCur has been significantly higher compared to Cur. The reduction of intracellular lipid was somewhat higher in the treatment with FCur compared to the treatment with Cur. (In all evaluations, p-value < 0.01 for curcumin and p-value < 0.001 for its derivative).
Conclusion: It seems that curcumin analog (FCur) can be used as a medicinal supplement in the treatment of fatty liver, along with other common treatment methods, due to its wide range of pharmacological activities, including its effects on lipid metabolism in the liver.
