Showing 4 results for Placenta
Md F Yasaie, Ma M Ghorbani,
Volume 30, Issue 3 (9-2006)
Abstract
Abstract
Background: Vaginal bleeding is a tragedy that causes a high mortality and morbidity rate. The aim of this study was to find the most frequent causes of vaginal bleeding in first half of pregnancy.
Materials and methods: This study is a cross sectional study performed on 161 cases with vaginal bleeding during pregnancy who had been referred to Taleghani Hospital. Sampling was achieved using non random convenient technique.
Results: The mean age of subjects was 27.1±6.1 years and the mean gestational age was 16.3±10.1 weeks. Mean of gravidity was 2.5±1.6. Thirty three percents of subjects were primigravid. The most common causes of bleeding were: abortion (72.7%), placenta previa (12.4%), ectopic pregnancy (8.1%), and placenta abruptio (6.8%).
Conclusion: Of 161 cases, 75 (46.5%) lead to abortion or curettage. Abortion was by far the most common cause of vaginal bleeding
Meraj Tabatabaei, Amir Hossein Zarnani, Shohreh Nikoo, Bahareh Keshavarzi, Fahimeh Ramzani Tehrani, Soheila Arefi, Ebrahim Mirzadegan, Nariman Mosaffa,
Volume 37, Issue 1 (4-2013)
Abstract
Background: Human amniotic epithelial cells (hAECs) have been introduced as a valuable source of stem cells with therapeutic applications. Despite the extensive study and emerging evidence, cell surface marker signature of hAECs remained controversial. The aim of the present study was to establish an efficient and optimized isolation procedure of hAECs and their characterization in terms of proliferation and stem cell markers.
Materials and methods: Four human placentas were collected from healthy women undergoing elective caesarian delivery at term labor under sterile condition. Amniotic membranes were carefully isolated and subjected to trypsin digestion. Single cell suspensions were obtained and the epithelial origin was confirmed by assessment of cytokeratin expression. Expression of CD9, CD10, CD29, CD34, CD38, CD44, CD45, CD73, CD105, CD133, MHC-I, HLA-DR, HLA-G, SSEA-4, STRO-1 and OCT-4 was then evaluated by flow cytometry.
Results: The overall yield of hAEC ranged from 80-130×106 cells per placenta with more than 98% viability. Microscopic examination revealed that hAECs are large and refractive cells with great capacity to adhere to plastic surfaces. The cells substantially expressed cytokeratin implying their epithelial origin. Flow cytometry data revealed that hAECs are a heterogenic population consisting of immune phenotypically mixed cells. Nearly all purified cells expressed mesenchymal markers, CD9, CD10, CD29, and CD73. A large proportion of cells also expressed HLA-I, HLA-G, STRO-1, OCT-4 and SSEA-4. Isolated cells, however, failed to express CD34, CD38, CD44, CD45, CD105, CD133 and HLA-DR.
Conclusion: The procedure presented here is a simple and cost-effective protocol for isolation of a large numbers of viable hAECs. Surface markers and such features as proliferation capacity of hAECs are among the characteristics that are strongly dependent upon isolation and cell culture conditions. Thus such variables should be taken in to consideration when stem cell properties of hAECs are to be interpreted.
Parasto Gholami, Sarvin Bagheralmoosavi, Dr. Shirin Kian Ersi, Dr. Mohammad Hojjat-Farsangi, Dr. Sina Salari, Dr. Marjan Yaghmaie, Dr. Jafar Mahmoudian, Dr. Mahmood Jeddi-Tehrani, Dr. Hossein Asgarian-Omran, Dr. Amirhasan Zarnani, Dr. Mahdi Shabani,
Volume 45, Issue 2 (6-2021)
Abstract
Purpose: Placenta-specific protein 1 (PLAC1) is one of the members of cancer-testis antigens family that has limited expression in normal tissue, but is upregulated in a variety of malignant tissues. Considering the lack of studies on the expression of FCRL1 in CML and CLL, the current study was conducted to examine the expression pattern of PLAC1 gene in these leukemias.
Materials and Methods: Fresh peripheral blood samples were collected from 6 CML and 10 CLL patients. In addition, peripheral blood samples of 10 healthy individuals were collected in EDTA as control group. All patients and healthy individuals signed a consent letter before sampling. The mononuclear cells were separated using ficoll-hypaque gradient centrifugation. Isolated mononuclear cells were used for RNA extraction and cDNA synthesis using RT-PCR method. Then, PLAC1 transcript expression in comparison to GAPDH were detected via Real-Time PCR. The statistical analyses were performed using chi-square test in SPSS.
Results: All 10 normal samples were negative for PLAC1. The PLAC1 expression was found to be statistically different in CML group (4 out of 6 cases) compared with that in the normal group (Pvalue = 0.000). However, CLL revealed no significant difference compared to normal individuals for PLAC1 expression (P Value = 0.648). In a significant percentage of CML patients, PLAC1 expression was positive but in CLL patients PLAC1, transcript expression was not evident.
Conclusion: It seems that PLAC1 could potentially be proposed as a biomarker in CML to aid in the diagnosis, prognosis, and treatment in the future.
Ms Asal Honarpour, Dr Ahmad Majd, Dr Reza Mirfakhraie, Mr Seyed Hamid Jamaldini, Ms Maryam Rahimi, Dr Sina Salimi Nasab,
Volume 48, Issue 3 (12-2024)
Abstract
Background and Aim: Preeclampsia is a significant condition during pregnancy, and if left untreated, it increases the risk of maternal and fetal mortality. Abnormal placentation is widely recognized as the primary cause of this disorder. Increased expression of Activin A, which plays a crucial role in placental and fetal development, has been confirmed in patients with preeclampsia. However, the mechanism of action of the Activin A receptor (ACVR2A) remains unclear. Therefore, this study examined the expression of the ACVR2A gene in the placentas of women with preeclampsia compared to those of healthy pregnant women.
Methods: In this case- control study, the expression of the ACVR2A gene in 40 placental samples, including 20 from women with preeclampsia and 20 from healthy pregnant women, was analyzed using quantitative Real- Time PCR (qPCR). The expression changes between the two groups were assessed with REST software, and the expression variation charts were plotted using T-test and Mann- Whitney statistical tests in GraphPad Prism software.
Results: increased expression of the ACVR2A gene was observed in the placentas of women with preeclampsia compared to normal placentas . The placenta in the preeclampsia group showed a 5.5-fold increase in gene expression compared to the control group.
Conclusion: It appears that the upregulation of ACVR2A gene expression plays a role in the development of preeclampsia.
