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Islamic Azad University, Science and Research Branch, Tehran , naji_mahtab@yahoo.com
Abstract:   (512 Views)
Background and Objective: Soybean protein is a major component of the diet of some people and has recently been increasingly considered in human diet. Soybeans have a lot of effects on human health. In fact, the use of useful microorganisms for the production of food has started centuries ago. Useful lactobacilli are useful microorganisms. The purpose of this study was study on the antimicrobial of lactobacilli isolated from soya on E. coli and Staphylococcus aureus.
Materials and Methods: At first, different brands of soybeans were purchased. Then one .10 grams of it was injected into 50 ml of water. After a night, in MRS agar culture was cultured.Then catalase and oxidase tests were performed on the colonies that appeared. Fermentation test of different sugars and antimicrobial properties of isolated bacteria on Escherichia coli and Staphylococcus aureus were investigated. For molecular studies, DNA was extracted, with specific primers, PCR was performed. Subsequently, the PCR product was taken on an electrophoresis gel, and after being observed, the gene bands were photographed.
Results: Of 32 Lactobacillus isolated from 16 different soybean brands (the highest frequency was related to Lactobacillus plantarum with 21.8% and the lowest frequency of Lactobacillus casei and Lactobacillus pantherus each with 3.125%), all of which have the property Antimicrobial against E. coli and Staphylococcus aureus. The lactobacillus plantarum bacterium had a greater no growth than the rest of the species. All samples tested in the PCR test form gene bands.
Discussion: While using soybeans that have beneficial nutritional benefits for humans, they contain lactobacilli which have antimicrobial properties, can be used in food and pharmaceutical industries for the health of humans and animals.
Type of Study: Original | Subject: Microbiology
Received: 2019/06/30 | Accepted: 2020/10/14

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