Volume 48, Issue 2 (8-2024)
Research in Medicine 2024, 48(2): 37-49 |
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Ethics code: IR.IAU.SRB.REC.1400.241
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Olyaiee A, Yadegar A, Mirsamadi E S, Sadeghi A, Mirjalali H. Study of the Role of Blastocystis sp. on Firmicutes and Bacteroidetes in IBS Patients. Research in Medicine 2024; 48 (2) :37-49
URL: http://pejouhesh.sbmu.ac.ir/article-1-3367-en.html
URL: http://pejouhesh.sbmu.ac.ir/article-1-3367-en.html
Foodborne and Waterborne Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran. , hamed_mirjalali@hotmail.com
Abstract: (191 Views)
Background and Aim: Irritable bowel syndrome (IBS) is a prevalent gastrointestinal (GI) tract disorder. Although the main reason for IBS is unclear, the interaction between intestinal microorganisms and the gut barrier seems to play an important role in the pathogenesis of IBS. The current study aimed to investigate the effect of Blastocystis on the Firmicutes/ Bacteroidetes ratio of IBS patients compared to healthy subjects.
Methods: This is a case- control study. Stool samples were collected from 80 participants, including 40 samples from each IBS and healthy group. Upon DNA extraction from stool samples, barcoding region and quantitative real- time polymerase chain reaction (PCR) were analyzed to investigate Blastocystis and the Firmicutes/ Bacteroidetes ratio, respectively. Student T-test, Shapiro- Wilk, Mann- Whitney, and Wilcoxon were employed to analyze data.
Results: The value of Firmicutes was increased in IBS patients (14.52 ± 3.04) compared to healthy controls (12.83 ± 2.19). Regarding Blastocystis, Firmicutes were higher than other groups (14.238 ± 3.836). The value of Bacteroidetes in IBS patients (8.11 ± 1.69) was higher than healthy controls (7.62 ± 1.62). Regarding Blastocystis, the changes in IBS patients with Blastocystis were higher than other groups (8.27 ± 1.79). The changes in the Firmicutes/ Bacteroidetes ratio were not significant in different groups.
Conclusion: The present study revealed that the changes in bacteria were not significant between IBS and healthy controls. In addition, Blastocystis does not change the abundance of bacteria in IBS and healthy subjects. More studies are needed to clear the effect of Blastocystis on gut microbiota.
Methods: This is a case- control study. Stool samples were collected from 80 participants, including 40 samples from each IBS and healthy group. Upon DNA extraction from stool samples, barcoding region and quantitative real- time polymerase chain reaction (PCR) were analyzed to investigate Blastocystis and the Firmicutes/ Bacteroidetes ratio, respectively. Student T-test, Shapiro- Wilk, Mann- Whitney, and Wilcoxon were employed to analyze data.
Results: The value of Firmicutes was increased in IBS patients (14.52 ± 3.04) compared to healthy controls (12.83 ± 2.19). Regarding Blastocystis, Firmicutes were higher than other groups (14.238 ± 3.836). The value of Bacteroidetes in IBS patients (8.11 ± 1.69) was higher than healthy controls (7.62 ± 1.62). Regarding Blastocystis, the changes in IBS patients with Blastocystis were higher than other groups (8.27 ± 1.79). The changes in the Firmicutes/ Bacteroidetes ratio were not significant in different groups.
Conclusion: The present study revealed that the changes in bacteria were not significant between IBS and healthy controls. In addition, Blastocystis does not change the abundance of bacteria in IBS and healthy subjects. More studies are needed to clear the effect of Blastocystis on gut microbiota.
Type of Study: Original |
Subject:
Parasitology
Received: 2024/02/20 | Accepted: 2024/06/11 | Published: 2024/09/16
Received: 2024/02/20 | Accepted: 2024/06/11 | Published: 2024/09/16
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