Volume 45, Issue 1 (3-2021)
Research in Medicine 2021, 45(1): 34-40 |
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nabavinia G, tehranipour M, Baharara J. Effect of hydroalcoholic extract and ethyl acetate and N-butanol fractions of Agrimonia eupatoria plant on the overgrowth of anterior spinal cord alpha neurons after sciatic nerve compression in male rats. Research in Medicine 2021; 45 (1) :34-40
URL: http://pejouhesh.sbmu.ac.ir/article-1-2047-en.html
URL: http://pejouhesh.sbmu.ac.ir/article-1-2047-en.html
Department of Biology, Mashhad Branch, Islamic Azad University , maryam_tehranipour@mshdiau.ac
Abstract: (1548 Views)
Denervation or compression result in the destruction of the cell body of neurons in the anterior horn of the spinal cord. Environmental nerve damage in the form of retrograde affects the cell body of alpha neurons and causes central degeneration of neurons in the spinal cord. Agrimonia eupatoria from the Golsorkhian family has antioxidant and anti-inflammatory effects and is effective in the survival and division of neurons. Therefore, the present study was conducted with the aim of determining the neuroprotective effects of hydroalcoholic extract of the plant unaffected by neural density after compression of the sciatic nerve in rats.
Methods: In the present experimental study, a total of 36 male Wistar rats weighing 250-300 g were randomly divided into 6 groups of 6: control, compression, treatment A (compression + extract at 75 mg / kg dose), treatment B (Compression + ethyl acetate fraction at 75 mg / kg dose), C treatment (compression + N-butanol fraction at 75 mg / kg dose), and D treatment (compression + blue phase with 75 mg / kg dose). Ghafath plant hydroalcoholic extract was prepared using Souxelle method. In the control group, after anesthetizing the rats, the muscle in the sciatic nerve was split without nerve damage and in the compression group and the sciatic nerve treatment group, the right leg was compressed for 30 seconds. The first intravenous infusion was performed immediately after nerve compression and the second infusion was performed seven days later. After 28 days, the rats were subjected to perfusion and then the lumbar spinal cords were sampled, and the samples were serialized after the tissue passage and blockage steps and stained with blue toluidin and erythrosine. Neurotic densities were calculated using directory method and the results were compared running t-test and ANOVA.
Results: The results of comparing neuronal densities between compression and treatment groups showed that all the treatment groups had a significant difference in neuronal density with compression group (P <0.001). Maximum neuronal density was related to n-butanol with a mean of (1864 ± 34), then the group alcoholic ( mean: 1614 ± 29) and the aqueous phase (1597 ± 24) and the Department of ethyl acetate with a mean of 1292 ±33.
Conclusion: It seems that the hydroalcoholic extract of Ghafas plant increases the density of the anterior alpha branch of the spinal cord after compression of the sciatic nerve in male rats, which may be due to compounds such as tocopherol, and with its antioxidant and anti-apoptotic effects can be effective in repairing the nervous system.
Methods: In the present experimental study, a total of 36 male Wistar rats weighing 250-300 g were randomly divided into 6 groups of 6: control, compression, treatment A (compression + extract at 75 mg / kg dose), treatment B (Compression + ethyl acetate fraction at 75 mg / kg dose), C treatment (compression + N-butanol fraction at 75 mg / kg dose), and D treatment (compression + blue phase with 75 mg / kg dose). Ghafath plant hydroalcoholic extract was prepared using Souxelle method. In the control group, after anesthetizing the rats, the muscle in the sciatic nerve was split without nerve damage and in the compression group and the sciatic nerve treatment group, the right leg was compressed for 30 seconds. The first intravenous infusion was performed immediately after nerve compression and the second infusion was performed seven days later. After 28 days, the rats were subjected to perfusion and then the lumbar spinal cords were sampled, and the samples were serialized after the tissue passage and blockage steps and stained with blue toluidin and erythrosine. Neurotic densities were calculated using directory method and the results were compared running t-test and ANOVA.
Results: The results of comparing neuronal densities between compression and treatment groups showed that all the treatment groups had a significant difference in neuronal density with compression group (P <0.001). Maximum neuronal density was related to n-butanol with a mean of (1864 ± 34), then the group alcoholic ( mean: 1614 ± 29) and the aqueous phase (1597 ± 24) and the Department of ethyl acetate with a mean of 1292 ±33.
Conclusion: It seems that the hydroalcoholic extract of Ghafas plant increases the density of the anterior alpha branch of the spinal cord after compression of the sciatic nerve in male rats, which may be due to compounds such as tocopherol, and with its antioxidant and anti-apoptotic effects can be effective in repairing the nervous system.
Type of Study: Original |
Subject:
Physilogy
Received: 2019/06/1 | Accepted: 2020/07/11 | Published: 2021/10/30
Received: 2019/06/1 | Accepted: 2020/07/11 | Published: 2021/10/30
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