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Production and research complex of Pasteur Institute of Iran , mparyan@gmail.com
Abstract:   (558 Views)
Background: Neisseria meningitidis is a leading cause of children death in developed countries. Meningococcus has 13 sero-groups based on the capsule antigens. Rapid diagnosis of the bacteria results in timely selection of the right anti-microbial treatment. This research which was conducted in 2019, aimed to produce a highly pure antiserum for diagnostic assays for the detection of meningococcus sero-groups.
Methods: The type of research study was research and development. Inactivated bacterial suspension was intravenously injected into white New Zealand Rabbits. Serum was collected after the last injection. Purification process was performed using precipitation, chromatography, and tangential flow filtration. The produced antiserum was used in Agglutination and ELISA assays. The purified antiserum had a purity of more than 98% at a suitable quantity. Agglutination test with the primary antigen showed 4+ result. To developed the ELISA assay, the optimized concentration of primary and biotinylated antibodies were 1 and 80 microgram, respectively. The results of positive predictive value was 100% and Negative predictive value was 96%, for the designed assay.
Conclusion: serum-based assays are recommended for the timely diagnosis of the disease since these assays are specific, sensitive, inexpensive, and rapid. The antiserum produced in this research is highly pure. Therefore, we recommend the antiserum for the development of agglutination and ELISA assays for the detection of N. meningitides.
     
Type of Study: Applicable | Subject: Immunology
Received: 2021/03/16 | Accepted: 2021/12/1

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