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Science and Research Branch, Islamic Azad University, Tehran , eidi@srbiau.ac.ir
Abstract:   (227 Views)
Background & aim: Gastric ulcer is a chronic gastrointestinal disease that is caused by the use of nonsteroidal anti-inflammatory drugs (NSAIDs) that increase the risk of gastrointestinal complications such as ulcers or bleeding. The use of antioxidants as adjunctive therapy for this disease is very important. Pantothenic acid is a B vitamin and a powerful antioxidant that plays an important role in the metabolism of carbohydrates, fats, and proteins in the body. The aim of this study was to evaluate the effect of pantothenic acid on antioxidant factors in improving experimental models of indomethacin-induced gastric ulcers in NMRI mice.
Materials and Methods: In this experimental study, 60 adult male NMRI mice (bodyweight 25-30 g) were randomly divided into 10 groups. Mice in the control group were intact. The sham group received pantothenic acid solvent (normal saline). The experimental groups received pantothenic acid at doses of 100, 200, and 400 mg/kg, omeprazole (40 mg/kg) as a standard drug, and indomethacin (18 mg/kg) to induce ulcers by gavage for two weeks. Six hours after receiving indomethacin and wounding, the mice's stomachs were examined for macroscopic evaluation of the ulcer at the epithelial level, visible lesions, inflammation with bleeding and edema, as well as biochemical evaluation of antioxidant enzymes by light absorption with an ELISA device. Statistical data were analyzed by one-way ANOVA.
Results: Administration of indomethacin significantly reduces gastric antioxidants such as superoxide dismutase (SOD), glutathione peroxidase (GPx), and catalase (CAT) activity (p<0.001), and increases malondialdehyde (MDA) levels compared with the normal control group (p<0.001). Treatment with pantothenic acid significantly increased the amount of SOD and CAT with doses of 200 (p<0.01), 400 mg/kg (p<0.001), and GPx in both doses (p<0.001) and the level of MDA with doses of 200 (p<0.05) and 400 mg/kg (p<0.001) significantly decreased. The result of pantothenic acid was 400 mg/kg equivalent to omeprazole and there is no significant difference between the two substances.
Conclusion: The results of this study suggest that pantothenic acid can protect the stomach against indomethacin-induced ulceration in mice.
     
Type of Study: Original | Subject: Physilogy
Received: 2021/07/27 | Accepted: 2022/03/6

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