Volume 48, Issue 3 (12-2024)
Research in Medicine 2024, 48(3): 40-51 |
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Ethics code: IR.SBMU.MSP.REC.1398. 770
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Dehbanipour R, Nikmanesh B, Eslami G, Hashemi A, Ghalavand Z. Study of Antibiotic Resistance Pattern and Mutation in Trm Gene of Acinetobacter Baumannii Isolated from Hospitalized Patients in Children's Medical Center in Tehran in 2019. Research in Medicine 2024; 48 (3) :40-51
URL: http://pejouhesh.sbmu.ac.ir/article-1-3388-en.html
URL: http://pejouhesh.sbmu.ac.ir/article-1-3388-en.html
Department of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran. , zghalavand@sbmu.ac.ir
Abstract: (306 Views)
Background and Aim: Acinetobacter baumannii is one of the most common causes of hospital infections, and infections caused by this organism cause high mortality in children. The emergence of resistance to last-line antibiotics in A. baumannii is worrying. The aim of this study was to determine the pattern of antibiotic resistance and investigate the mutations in the trm gene, as a resistance mechanism, in tigecycline- resistant isolates obtained from children.
Methods: In this cross- sectional- descriptive study, A. baumannii isolates were collected from Children's Medical Center in Tehran, Iran during a one- year period. Definitive identification of the isolates was done by identifying blaOXA-51-like and rpoB genes by polymerase chain reaction (PCR). Then, antimicrobial susceptibility testing of the isolates was assessed using Kirby- Bauer disc diffusion method. Minimum inhibitory concentration (MIC) of colistin and tigecycline were determined by broth microdilution test and E-test, respectively. The presence of trm gene was measured by PCR. Investigation of possible mutations in trm gene was specified by DNA sequencing in tigecycline- resistant isolates.
Results: In this study, 67 clinical isolates of A. baumannii were collected. All isolates were sensitive to colistin. Three isolates (4.4%) were resistant to tigecycline with MIC= 8 mg/l and 13 isolates were moderately sensitive to tigecycline. DNA sequencing results of 3 tigecycline resistant isolates showed mutation in trm gene in all three isolates.
Conclusion: Since tigecycline is a drug of last resort antimicrobials for the treatment of MDR A. baumannii isolates, this growing rate of resistance will make treatment difficult. This emphasizes the necessity of further investigation of resistance mechanisms in order to prevent the emergence of more resistance. The results obtained from this study indicate the important role of mutation in the trm gene in the resistance of A. baumannii to tigecycline.
Methods: In this cross- sectional- descriptive study, A. baumannii isolates were collected from Children's Medical Center in Tehran, Iran during a one- year period. Definitive identification of the isolates was done by identifying blaOXA-51-like and rpoB genes by polymerase chain reaction (PCR). Then, antimicrobial susceptibility testing of the isolates was assessed using Kirby- Bauer disc diffusion method. Minimum inhibitory concentration (MIC) of colistin and tigecycline were determined by broth microdilution test and E-test, respectively. The presence of trm gene was measured by PCR. Investigation of possible mutations in trm gene was specified by DNA sequencing in tigecycline- resistant isolates.
Results: In this study, 67 clinical isolates of A. baumannii were collected. All isolates were sensitive to colistin. Three isolates (4.4%) were resistant to tigecycline with MIC= 8 mg/l and 13 isolates were moderately sensitive to tigecycline. DNA sequencing results of 3 tigecycline resistant isolates showed mutation in trm gene in all three isolates.
Conclusion: Since tigecycline is a drug of last resort antimicrobials for the treatment of MDR A. baumannii isolates, this growing rate of resistance will make treatment difficult. This emphasizes the necessity of further investigation of resistance mechanisms in order to prevent the emergence of more resistance. The results obtained from this study indicate the important role of mutation in the trm gene in the resistance of A. baumannii to tigecycline.
Type of Study: Original |
Subject:
Microbiology
Received: 2024/06/16 | Accepted: 2024/08/11 | Published: 2025/01/20
Received: 2024/06/16 | Accepted: 2024/08/11 | Published: 2025/01/20
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