Volume 49, Issue 2 (9-2025)
Research in Medicine 2025, 49(2): 0-0 |
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Ethics code: IR.SBMU.MSP.REC.1399.158
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Dehghan G, Bazrgaran A, Faghihloo E. Prevalence of Cytomegalovirus (CMV) and Epstein–Barr Virus (EBV) in the Cerebrospinal Fluid of Children with Encephalitis at Mofid Children’s Hospital, Tehran, in 1401(2022)-1402(2023). Research in Medicine 2025; 49 (2)
URL: http://pejouhesh.sbmu.ac.ir/article-1-3467-en.html
URL: http://pejouhesh.sbmu.ac.ir/article-1-3467-en.html
, faghihloo@gmail.com
Abstract: (13 Views)
Background and Aim:
Encephalitis, an inflammation of the brain parenchyma, is a serious and complex condition in children characterized by fever, headache, and altered consciousness. It can result in permanent brain damage, neurological disabilities, or even death. The causes of encephalitis are diverse, including infectious and non-infectious factors. Viruses such as Herpes Simplex Virus (HSV), Epstein-Barr Virus (EBV), and Cytomegalovirus (CMV) are common infectious agents that can directly or indirectly damage the brain. Early detection and treatment of these viral infections are crucial to reducing complications and preventing long-term sequelae.
Materials and Methods:
In this descriptive cross-sectional study, cerebrospinal fluid (CSF) samples from 45 children suspected of encephalitis were collected. After cell counting and CSF analysis, viral DNA was extracted using the High Pure PCR kit. DNA quality was confirmed by amplification of the GAPDH gene. Multiplex PCR was then performed to detect the presence of CMV and EBV in the samples. Statistical analysis was conducted using SPSS software.
Results:
Among the 45 CSF samples, 68.3% were from male and 31.7% from female patients. Molecular analysis revealed no presence of CMV in any sample, while one sample tested positive for EBV.
Conclusion:
These findings highlight the importance of rapid diagnosis and timely management of viral encephalitis. Further studies with larger sample sizes are recommended to better understand the viral etiology of encephalitis.
Encephalitis, an inflammation of the brain parenchyma, is a serious and complex condition in children characterized by fever, headache, and altered consciousness. It can result in permanent brain damage, neurological disabilities, or even death. The causes of encephalitis are diverse, including infectious and non-infectious factors. Viruses such as Herpes Simplex Virus (HSV), Epstein-Barr Virus (EBV), and Cytomegalovirus (CMV) are common infectious agents that can directly or indirectly damage the brain. Early detection and treatment of these viral infections are crucial to reducing complications and preventing long-term sequelae.
Materials and Methods:
In this descriptive cross-sectional study, cerebrospinal fluid (CSF) samples from 45 children suspected of encephalitis were collected. After cell counting and CSF analysis, viral DNA was extracted using the High Pure PCR kit. DNA quality was confirmed by amplification of the GAPDH gene. Multiplex PCR was then performed to detect the presence of CMV and EBV in the samples. Statistical analysis was conducted using SPSS software.
Results:
Among the 45 CSF samples, 68.3% were from male and 31.7% from female patients. Molecular analysis revealed no presence of CMV in any sample, while one sample tested positive for EBV.
Conclusion:
These findings highlight the importance of rapid diagnosis and timely management of viral encephalitis. Further studies with larger sample sizes are recommended to better understand the viral etiology of encephalitis.
Type of Study: Original |
Subject:
Virology
Received: 2025/06/8 | Accepted: 2025/10/18 | Published: 2025/12/13
Received: 2025/06/8 | Accepted: 2025/10/18 | Published: 2025/12/13
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