, Hedayati@endocrine.ac.ir
Abstract: (15478 Views)
Abstract
Background: Inactivation of tumor suppressor genes can cause irreversible uncontrolled growth. PTEN is a tumor suppressor gene and its polymorphism (IVS4) role in breast cancer was investigated in this study.
Methods: Forty nine female subjects with confirmed breast cancer comprised the case group and 43 healthy women participated as a control group in this study. The PTEN polymorphism was genotyped by amplification of genomic DNA and then digestion by restriction of endonuclease and finally RFLP analysis.
Results: The frequencies of mutant allele (M) were 0.37 and 0.27, respectively in the case and control groups. The genotype frequency distributions were in Hardy-Weinberg equilibrium in both groups.
Conclusion: The mutant allele frequency in case group was 10% higher than control group it is possible that PTEN IVS4 polymorphism has a role in Breast Cancer.
KEYWORDS: PTEN, IVS4, Breast Cancer, polymorphism.
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