Volume 35, Issue 2 (6-2011)                   Research in Medicine 2011, 35(2): 93-98 | Back to browse issues page

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Behdani M, Karimipour M, Khanahmad Shahreza H, Asadzadeh N, Azadmanesh K, Khabiri A, et al . Production of camel polyclonal antibody against vascular endothelial growth factor receptor-2 and performance evaluation in ELISA test. Research in Medicine. 2011; 35 (2) :93-98
URL: http://pejouhesh.sbmu.ac.ir/article-1-887-en.html
Department of Molecular Medicine, Pasteur Institute of Iran, Tehran, Iran , sirouszeinali@yahoo.com
Abstract:   (11366 Views)
Abstract Background: In molecular approach, serum of camel contains a unique type of antibodies devoid of light chains since the light chain is missing, the heavy-chain antibodies should bind their antigen by one single domain, the variable domain of the heavy immunoglobulin chain. Vascular endothelial growth factor receptor-2 (VEGFR-2) is one of the important proteins in angiogenesis which over-expressed in many types of tumors. Methods: Two male dromedaries (Camelus dromedarius) were immunized against VEGFR-2. ELISA was used to evaluate the immunization process. Camel immune sera were fractionated with protein A and G affinity chromatography. Heavy chain antibodies tested for its specific reactivity in cell-based ELISA in recognition of VEGFR-2 on the cell surface of three cell lines 293/KDR, HUVECs and A431. Results: In ELISA test, the camel sera in 1/12800 dilution had the acceptable results. Furthermore, cell-based ELISA demonstrated that the polyclonal heavy chain antibodies bind to VEGFR-2 in 293/KDR and HUVECs cell lines.Conclusion: Single chain polyclonal antibodies against VEGFR-2 can be used for detection of soluble form of this protein by ELISA, and also flowcytometry, western blotting and immunohistochemistry. Keywords: Camel, Single chain antibody, Polyclonal antibody, VEGFR-2.
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Type of Study: Original | Subject: Interdisciplinary (Educational Management, Educational research, Statistics, Medical education
Received: 2011/09/27 | Published: 2011/06/15

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