Volume 21, Issue 4 (Mars 1997)
Research in Medicine 1997, 21(4): 80-90 |
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Ne'amatollahi N, Rezuadeh M, Hoseini A, Mazdarani H. The Effect Of Vero Cells on Preimplantation Mouse Embryo in Cultur Medium. Research in Medicine 1997; 21 (4) :80-90
URL: http://pejouhesh.sbmu.ac.ir/article-1-2293-en.html
URL: http://pejouhesh.sbmu.ac.ir/article-1-2293-en.html
Abstract: (1310 Views)
SUMMARY
To study the effect of vero cells on preimplantation mouse embryos two groups of experiment were carried out. In experiment 1, pronuclear stage mouse embryos were co-cultured with Vero cells in RPMI medium and were examined under microscope for 4 days with 24h interval. The results showed co-cultured group developed better compared to control (87% to 80%, P<0.05) although, the rate of development to blastocyst was relatively similar (8% to 7%).In experiment 2 two-cell mouse embryos were co-cultured with Vero cells. 72h. after cultivation more co-cultured embryos developed to expanded blastocysts and moroulae (80% to 75%, P<0.05) in addition, more embryos in co-cultured group hatched (48% to 27%, P<0.001).
We conclude that although the Vero cell is able to over come the two-cell block but supports the embryos which passed this block invivo.
Type of Study: Original |
Subject:
Microbiology
Received: 2020/02/22 | Accepted: 2020/02/22 | Published: 2020/02/22
Received: 2020/02/22 | Accepted: 2020/02/22 | Published: 2020/02/22
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