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Department of Biology, Mashhad Branch, Islamic Azad University. , maryam_tehranipour@mshdiau.ac.ir
Abstract:   (199 Views)
Background: Memory and learning are the most important behavioral processes that occur at the highest functional levels of the central nervous system. Probiotics reduce oxidative stress by reducing inflammatory damage and increasing levels of antioxidant enzymes such as superoxide dismutase and glutathione peroxidase. Therefore, the aim of this study was to investigate the effect of the probiotic Bifido bacterium bifidum on the neuronal density of the hippocampus in Alzheimer's rats.
Methods: In this experimental study, 30 male Wistar rats weighing about 200 to 250 g were prepared and randomly divided into 5 control groups, Alzheimer's and 3 treatment groups. To induce Alzheimer's, streptozotocin was injected at a dose of 5 mg / kg with a volume of 5 μl in each lateral ventricle of mice by stereotaxic method. In the treatment groups, probiotics were injected intraperitoneally with doses of 10-10 ml, 10-10, 7-10, 8-10 mg / CF simultaneously with STZ injection for 21 days. After 21 days, the rats treated with rampon and ketamine were anesthetized and after perfusion method, the brain was removed from the skull and placed in 10% saline formalin. After tissue passage, seven-micron sections were prepared from the brain and stained with toluidine blue and erythrosine. CA1, CA2, CA3 DG regions were imaged and neuronal density was calculated using a dissector and stereology method and the results of the groups were compared using ANOVA.
Results: The results of this study showed that in the groups treated with the probiotic Bifido bacterium bifidum in all regions of the hippocampus, the neuronal density increased compared to the Alzheimer's group (p <0.001).
Conclusion: It seems that the probiotic Bifido bacterium bifidum with its anti-inflammatory and antioxidant effects has been able to prevent nervous system lesions.
Type of Study: Original | Subject: Physilogy
Received: 2020/07/31 | Accepted: 2022/04/20

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