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Iranian Biological Resource Center , ashouri_sepideh@yahoo.com
Abstract:   (186 Views)
Background and Aim: In males, spermatogonial stem cells (SSCs) are the cause of germ cell production and therefore fertility. In vitro culture of SSCs, while providing suitable conditions for further studies on the process of spermatogenesis, can be a solution for the treatment of infertility in men. Culturing cells in a three-dimensional substrate creates better conditions for cell interaction than in a two-dimensional substrate and is closer to body conditions. In this study, decellularized testicular tissue (DTM) of rats was used to create a three-dimensional substrate similar to body conditions.
Materials and Methods: After extraction of SSCs by enzymatic method from testicular tissue of newborn mice, these cells were propagated in a specific culture medium for three weeks. After confirming the identity of the colonies resulting from the growth of these cells, they were cultured in two groups, one on a layer of DTM and the other in two-dimensional conditions of conventional culture dishes with differential culture medium. In the fourth week since the initiation of the differentiation culture, the expression of pre meiotic (OCT4 & PLZF) and meiotic (SCP3 & Protamine-2) genes were measured in both groups.
Results: The results showed that the expression of meiotic genes in cells cultured on DTM was significantly higher.
Conclusion: In DTM three-dimensional culture, due to the better communication of cells with each other and the presence of a natural extracellular matrix, more ideal conditions are created for the preservation, proliferation and differentiation of SSCs‌ than in two-dimensional culture.
     
Type of Study: Original | Subject: Anatomical sciences (anatomy, histology, embryology, reproductive biology)
Received: 2021/09/13 | Accepted: 2021/12/25

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