Research in Medicine

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Hatami Pirghibi T, Chehri K, Abiri R, Karimi I. Effect of hydro-alcoholic extracts of Rosmarinus officinalis L., Mentha piperita L., and Zataria multiflora Boiss on biofilm formation of Pseudomonas. Research in Medicine 2021; 45 (3) :11-17
URL: http://pejouhesh.sbmu.ac.ir/article-1-2179-en.html

Backgrond: Medicinal plants are promising therapeutic agents. Antimicrobial agents from plants are compounds that kill microorganisms or stop their  growth. The aim of the present study was to determine the effect of hydro-alcoholic extract of Rosmarinus officinalis, Zataria multiflora, and Mentha iperita on biofilm formation of Pseudomonas aeruginosa bacterium.
Materials and Methods: For the purpose of the present experimental study, extracts of these plants were prepared and their effects on P. aeruginosa growth were assessed using disk diffusion technique and the results were compared with disk diffusion results of kanamycin, imipenem, penicillin, and  cephalexin. Then,the Minimum Inhibitory Concentration (MIC) and the Minimum Bactericidal Concentration (MBC) of extracts were determined separately. The effect of these extracts on biofilm formation was assessed via staining with crystal violet %1 and acetic acid %30. All tests were repeated three times and statistical analyses were performed using SPSS, v. 16.
Results: We found that all three plant extracts at the concentration of 3 mg/ml had antimicrobial properties.The diameter of inhibition halos of extracts of R. officinalis, Z. multiflora, and M. piperita were 15 ,19, and 11 mm, respectively. The minimum inhibitory concentration of R. officinalis was 75/0 mg/ml and the minimum inhibitory concentration of Z. multiflora and M. piperita 5/1 mg/ml. Also, the results showed that R. officinalis reduces and inhibits Biofilm formation at concentrations of 5/1 and 3 mg/ml, respectively. But, Z. multiflora and M. piperita only reduced biofilm formation at concentration of 3 mg/ml.
Conclusion: It can be concluded that the alcoholic extract of R. officinalis with a concentration of 3 and 1.5 mg/ml is a potent inhibitor against P. aeruginosa biofilm formation.