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1- Department of biology, Faculty of Basic Science, Mashhad branch, Islamic Azad University, Mashhad, Iran , Email: J.khayatzadeh@mshdiau.ac.ir
Abstract:   (210 Views)

Background and purpose: Fertility in men includes the ability to produce a sufficient number of normal sperm, several factors affect the schermatogenesis-quality of sperm parameters. These factors include the use of certain chemical drugs, such as zinc and bioluxax-H (a combination of losartan + hydrochlorothiazide), common drugs used to treat hypertension. The present study investigated the effect of these drugs on spermatogenesis and the expression of Dogen on its effect in rat testis.
Materials and Methods
32 Wistar rats were randomly divided into four groups (n=8): GroupI (control) received distilled water; Group II (Lnh) treated with Lnh (losartan and hydrochlorothiazide )( 20/5 mg/kg BW/day; Group III (Zn) treated with the Zn (Zinc gluconate, 30mg/kg BW/day; Group IV (LnhZn) treated with Lnh and Zn at the same doses. Treatment was continued in all groups for 60 days. After the treatment, Testicular tissue, sperm parameters, AR and Dazl genes expression in the rats were investigated.
Results significant decrease was observed in the number of spermatogonia, spermatocyte, spermatid, sperm count in the Lnh compared to the control ,Although in the Zn group, all factors were similar to the control, even the number of spermatocytes, and  sertoli cell in this group increased compared to the control. Moreover, the Lnhzn group has shown similar results in all factors compared to the control.but in this group, an increase in spermatogonium compared to the control  expression level of AR and DAZL genes  decreased in Lnh. Also the expression of all three genes in the LnhZn  and Zn group similar to the control .
Conclusion: The use of zinc supplementation improved the side effects of Bioluxax-H on sperm quality
Key words: losartan-hydrochlorothiazide, Zinc, Spermatogenesis.
Type of Study: Original | Subject: Biology
Received: 2020/12/6 | Accepted: 2022/04/17

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