Volume 34, Issue 1 (3-2010)
Research in Medicine 2010, 34(1): 21-25 |
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Shabani1 P, Bandehpoor2 M, Emamgholipoor1 S, Maghen2 L, Seyed2 N, Yaghmaie1 B et al . Cloning of coagulant VII factor from hepatoma cell line. Research in Medicine 2010; 34 (1) :21-25
URL: http://pejouhesh.sbmu.ac.ir/article-1-724-en.html
URL: http://pejouhesh.sbmu.ac.ir/article-1-724-en.html
Parisa Shabani1 
, Mojgan Bandehpoor2 , Salaleh Emamgholipoor1 , Leila Maghen2 , Negar Seyed2 , Bahram Yaghmaie1 , Bahram Kazemi2
, Mojgan Bandehpoor2 , Salaleh Emamgholipoor1 , Leila Maghen2 , Negar Seyed2 , Bahram Yaghmaie1 , Bahram Kazemi2
, : bahram_14@yahoo.com
Abstract: (11140 Views)
Abstract
Background: Factor VII, is a coagulant protease it begins the proteolytic cascade reactions and produces thrombin. The use of recombinant human factor VII, (rhFVII) is effective for the treatment of patients with hemophilia A or B. It is a target for gene therapy. This study was done to clone factor VII from HepG2 cell line.
Methods: RNA was extracted from the hepatoma, (HepG2), cell line. On reverse transcription FVII cDNA was amplified by RT-PCR. PCR product was cloned into the pTZ57R/T vector and transported into the E-coli cells.
Results: By amplification of the FVII gene, the PCR band was observed and cloning into the vector was confirmed by restriction analysis.
Conclusion: In this paper we report the cloning of factor VII from HepG2 cell line.
Keywords: Cloning, Coagulant VII factor, Hepatoma cell line.
Type of Study: Original |
Received: 2010/09/27 | Published: 2010/03/15
Received: 2010/09/27 | Published: 2010/03/15
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