Volume 40, Issue 3 (11-2016)                   Research in Medicine 2016, 40(3): 149-154 | Back to browse issues page

XML Persian Abstract Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Narimani R, Moghaddam M. Investigation of antibacterial properties of ethanolic extracts of four native medicinal plants of Ardebil province by two methods of disk and well diffusion. Research in Medicine. 2016; 40 (3) :149-154
URL: http://pejouhesh.sbmu.ac.ir/article-1-1597-en.html
Ferdowsi University of Mashhad , m.moghadam@um.ac.ir
Abstract:   (4847 Views)

Background & Objectives: Resistance to antibiotic and also the side effects of drug consumption are the main problem in antibiotic treatment. Therefore, after doing researches on the plants properties, humankind has attempted to use them in different industries. In this investigation the anti-microbial effects of ethanolic extracts of four medicinal plants from Ardebil province include Nepeta nuda, Salvia verticilata, Nepeta crassifolia and Teocrium chamaedry subsp. syspirense, on three species of gram negative bacteria (Salmonella enteric, Escherichia coli, Pectobacterium carotovorum) were evaluated.

Material and Methods: An experimental study was conducted at in vitro conditions. After preparing ethanolic extract of mentioned plants by maceration method, the antimicrobial effect of outcome extracts from their aerial parts were investigated by two different methods: disk diffusion and well diffusion.

Results: The highest growth inhibition zone in E. coli، P. caratovorum، S. enterica bacteria at 800 mL/mg concentration in disk diffusion method (12, 12, 12 mm) at N. crassifolia, N. nuda, and N. crassifolia extracts and in well diffusion method (10, 12 and 11 mm) at S. verticilata, N. nuda and N. crassifolia extracts were seen respectively.

Conclusion: It seems that the different concentrations of extract and also bacterium type are influenced on growth inhibition zone. With increasing concentration, the inhibition zone is increased. In comparing to Gentamicin the extracts of mentioned plants had low effect on gram negative bacteria. 

Full-Text [PDF 617 kb]   (3482 Downloads)    
Type of Study: Original | Subject: Interdisciplinary (Educational Management, Educational research, Statistics, Medical education
Received: 2016/05/17 | Accepted: 2016/11/14 | Published: 2016/12/26

Send email to the article author

Creative Commons License
This Journal is licensed under a Creative Commons Attribution 4.0 International License  | Research in Medicine

Designed & Developed by : Yektaweb