Volume 48, Issue 1 (4-2024)
Research in Medicine 2024, 48(1): 50-62 |
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Ethics code: IR.IAU.MSHD.REC.1402.101
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Pourmohammad M, Khayatzadeh J, Azarizadeh M, Pouresmaeil V, Zafar Balanjad S. The Effect of the Hydroalcoholic Extract of Ferula Assa- Foetida Resin Root and Shoot on the VEGF and FGF Gene Expression in the Chorioallantoic Membrane of Chick Embryos. Research in Medicine 2024; 48 (1) :50-62
URL: http://pejouhesh.sbmu.ac.ir/article-1-3350-en.html
URL: http://pejouhesh.sbmu.ac.ir/article-1-3350-en.html
Mohammadreza Pourmohammad 
, Jina Khayatzadeh , Mostafa Azarizadeh , Vahid Pouresmaeil , Saeedeh Zafar Balanjad
, Jina Khayatzadeh , Mostafa Azarizadeh , Vahid Pouresmaeil , Saeedeh Zafar Balanjad
Department of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran. , J.khayatzadeh@mshdiau.ac.ir
Abstract: (1165 Views)
Background and Aim: Angiogenesis is the biological process of sprouting new vessels from existing vessels in the tissue. The main factor in the molecular guidance of this process is vascular endothelial growth factors (VEGF) and fibroblast growth factor (FGF). Considering the importance of plant medicine sciences and also, the role of angiogenesis in the processes such as wound healing, menstrual cycles, placental growth, and ovulation, the aim of this study was to investigate the effect of hydroalcoholic extract of the root and shoot of Ferula assa- foetida plant (as one of the prominent medicinal plants in traditional medicine) on changes in the gene expression of VEGF and FGF in the angiogenesis pathway of the chorioallantoic membrane (CAM) of chick embryos.
Methods: In this experimental research, 80 Ross fertilized eggs of the were randomly divided into 8 groups including the control group, laboratory control (pbs), and 6 experimental groups. On the eighth day of incubation, the laboratory control group was treated with normal saline and the experimental groups were treated with doses of 100, 200 and 300 µg/ml of the hydroalcoholic extract of the shoot and root of Ferula assa- foetida plant. On the twelfth day, a photo was taken of the chorioallantoic membrane to count the number of vessels and measure the length of the vessels. CAM samples were prepared for RNA extraction and cDNA production. The collected data were analyzed by t-test and ANOVA.
Results: The average number of vessels (26.1 ± 3.45) and vessel length (31.1 ± 0.78) in the treated group showed a significant decrease (P < 0.05) compared to the control group. Also, the gene expression level of VEGF and FGF in the treated group was significantly (P < 0.05) lower than the control group.
Conclusion: It seems that the hydroalcoholic extract of Ferula assa- foetida plant can affect the gene expression level of VEGF and FGF and the process of angiogenesis of the chorioallantoic membrane of chick embryos.
Methods: In this experimental research, 80 Ross fertilized eggs of the were randomly divided into 8 groups including the control group, laboratory control (pbs), and 6 experimental groups. On the eighth day of incubation, the laboratory control group was treated with normal saline and the experimental groups were treated with doses of 100, 200 and 300 µg/ml of the hydroalcoholic extract of the shoot and root of Ferula assa- foetida plant. On the twelfth day, a photo was taken of the chorioallantoic membrane to count the number of vessels and measure the length of the vessels. CAM samples were prepared for RNA extraction and cDNA production. The collected data were analyzed by t-test and ANOVA.
Results: The average number of vessels (26.1 ± 3.45) and vessel length (31.1 ± 0.78) in the treated group showed a significant decrease (P < 0.05) compared to the control group. Also, the gene expression level of VEGF and FGF in the treated group was significantly (P < 0.05) lower than the control group.
Conclusion: It seems that the hydroalcoholic extract of Ferula assa- foetida plant can affect the gene expression level of VEGF and FGF and the process of angiogenesis of the chorioallantoic membrane of chick embryos.
Keywords: Ferula assa- foetida resin, Hydroalcoholic extract, chorioallantoic membrane, angiogenesis, VEGF, FGF
Type of Study: Original |
Subject:
Biology
Received: 2023/12/18 | Accepted: 2024/03/3 | Published: 2024/04/22
Received: 2023/12/18 | Accepted: 2024/03/3 | Published: 2024/04/22
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