Research in Medicine

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Purpose: The staff members in ICU are the major carrier with different devices such as catheters. The aim of this study was to investigate the antibody reaction against immunogenic peptides of ompA designed as a candidate vaccine Against acinetobacter baumannii.
Material method: 62 serum samples obtained from exposed ICU staffs in Tehran Firoozabadi and Dezful Ganjavian hospitals and non-exposed individuals and individuals suffering with SLE. In the order to increase specificity and decrease non-specific anti body, All the serums were pre-adsorped with E-coli Lysate. In order to determine positive reaction of samples with Acinetobacter baumannii lysate due to detection of existence of antibodies against Acinetobacter baumannii.
ELISA technique helps us to all tests.
Results: obtained results showed that  from 33 samples of exposed serum, %75 of it was positive. Also from 22 samples of non-exposed individuals, %27 were positive. Strongly positive serum with lysate were reacted with only peptide 5 of ompA Acinetobacter baumannii.
Conclusion: obtained results shows that not only exposed samples had a lot more Ab titre in comparison to non-exposed. But also these groups had significant high tire of Abs against peptide 5.  In shows that the colonization of Acinetobacter baumannii in population is growing year to year. To demonstrate the exact role of antibody against Acinetobacter baumannii and immunologic peptides, there’s a need for further researches.

Keywords: nosocomial infection, Acinetobacter baumannii, outer membrane protein A, ELISA, immunogenic peptid

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