Volume 42, Issue 1 (3-2018)                   Research in Medicine 2018, 42(1): 46-52 | Back to browse issues page

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Poursafavi Z, Seyyed Tabaei S J, Kheirandish F, Mohebali M, Kamalinezhad M, Vajdian R. Effect of olive leaf aqueous and hydroalcoholic extract on Leishmania tropica Glucantime resistance and sensitivity in vitro. Research in Medicine. 2018; 42 (1) :46-52
URL: http://pejouhesh.sbmu.ac.ir/article-1-2021-en.html
Shahid Beheshti medical Scinces , zahrapoursafavi@yahoo.com
Abstract:   (2793 Views)
Background: Cutaneous Leishmaniasis is the most important parasitic disease. As the number of resistant
types to glucantime (drug of choice) is increasing, it is necessary to investigate the effects of other drugs,
like herbal medicines, on this disease. Several studies have shown the anti Leishmania effects of olive leaf
on the Leishmania and also some organisms. The aim of the present study was to evaluate the in vitro effects
of olive leaf aqueos and hydroalcoholic extract on amastigots of Leishmania tropica glutamimn resistance
and sensitivity in vitro.
Materials and Methods: Mouse peritoneal macrophages for the maintenance of Leishmania was used in
vitro culture. To determine the effect of the extract on macrophage, cytotoxicity MTT assay was performed
and CC50 was calculated. In order to investigate the Leishmania tropica amastigots glutamimn resistance
with different concentrations of olive leaf aqueous extract, i.e. 0.31, 0.625, 1.25, and 2.5mg/ml and olive leaf
hydroalcoholic extract with different concentrations of 3.1, 6.25, 12.5, 25 μg/ml were incubated for 24-72
hours. Geimsa stain was used as positive and negative controls, respectively.
Results: We found that 2/5mg/ml olive leaf aqueous extract and 25 μg/ml hydroalcoholic extract in 72
hours are the most effective concentration and time. It is important to note that olive leaf aqueous and
hydroalcoholic extract did not have any effect on Leishmania tropica Glucantime resistance in vitro (p≤0.05).
Conclusion: The natural products are available and safe and can be used as a drug model without side
effects, especially on amastigots of Leishmania tropica glutamimn sensitivity.
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Type of Study: Original | Subject: Parasitology
Received: 2019/04/22 | Accepted: 2019/04/22 | Published: 2019/04/22

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