Volume 44, Issue 4 (12-2020)                   Research in Medicine 2020, 44(4): 580-586 | Back to browse issues page

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Jamshidi H, Rahimi P, Moghaddam A. Effect of melatonin on SSCs colony formation in vitro. Research in Medicine. 2020; 44 (4) :580-586
URL: http://pejouhesh.sbmu.ac.ir/article-1-2087-en.html
Department of Clinical Sciences, Faculty of Veterinary Medicine, Razi University, Kermanshah, Iran , drp.rahimi@gmail.com
Abstract:   (1812 Views)
Background: Spermatogonial Stem Cells (SSCs) are used as an excellent model system for differentiation, development, and functioning of testes and provide a source of cells for infertility treatment and production of transgenic animals and recombinant drugs. The aim of the present study was to determine the effects of different concentrations of melatonin on prepubertal lamb’s spermatogonial colony formation.
 Methods: An experimental study was conducted. Each treatment was replicated 5 times. Testicular cells were isolated from testes of prepubertal lambs using two-step enzymatic digestion and then cultured for 10 days. Control cells (C) were grown in basic DMEM media with %1 antibiotic and %5 FBS. Treated cells were grown in basic media with 1 μmol/ml (T1) or 1 nmol/ml (T2) melatonin. Culture media was changed every 72h and SSCs colony formation was monitored via inverted microscope. Data were analyzed running one way ANOVA.
P values less than %5 were considered as statistically significant.
Results: SSCs were identified via immunocytochemistry assay against PGP9.5. Number and surface area of colonies were greater in T1 group than C and T2 groups on day 7 (P=0.04587).
Conclusion: The findings suggest that simultaneous addition of 1 μmol/ml melatonin to culture media may increase in vitro colony formation of prepubertal sheep SSCs. So, addition of above dose of melatonin to SSCsculture media is suggested.
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Type of Study: Original | Subject: Cellular Sciences (Molecular Cells, Stem Cells)
Received: 2019/08/25 | Accepted: 2019/12/10 | Published: 2020/06/27

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